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pRS410

Plasmid

#11258

Depositor

Fred Cross

Type

Empty backbone

Expression

Yeast

Available since

Feb. 24, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pBA900

Plasmid

#122237

Purpose

Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 incorporated at the 3' end of the sgRNA constant region.

Depositor

Jonathan Weissman

Publication

Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

Insert

sgRNA with cs2 at the 3' end of the constant region

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pORANGE Dlg4-GFP KI

Plasmid

#131477

Purpose

Endogenous tagging of PSD95: C-terminal (amino acid position: R721)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and GFP donor (Dlg4, Dlg4 Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-Ef1a-DIO hChR2(C128S/D156A)-EYFP

Plasmid

#35503

Purpose

AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Publication

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2(C128S/D156A)-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

C128S and D156A

Promoter

Ef1a

Available since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pTRE-Medium-FT

Plasmid

#31914

Depositor

Vladislav Verkhusha

Publication

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Medium-FT

Expression

Mammalian

Mutation

M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

Promoter

PTight Tet-responsive PminCMVd

Available since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti CMVtight Blast DEST (w762-1)

Plasmid

#26434

Depositor

Eric Campeau

Type

Empty backbone

Use

Lentiviral; Tet-on advanced destination vector

Expression

Mammalian

Available since

Jan. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA-SET-FLAG-HA

Plasmid

#24998

Depositor

Judy Lieberman

Publication

Yan et al PLoS Pathog. 2009 Mar . 5(3):e1000327.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SET (SET Human)

Tags

FLAG and HA

Expression

Mammalian

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBR322-PVCpnf17-22

Plasmid

#198272

Purpose

Unmodified pPayload; contains WT PVC payloads/regulatory genes

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf17-22

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CaMKIIP-mEGFP-P2A-paAIP2/pAAV

Plasmid

#91718

Purpose

AAV-mediated expression of mEGFP and paAIP2 for optogenetic control of CaMKII

Depositor

Ryohei Yasuda

Publication

Murakoshi et al Neuron. 2017 Apr 5;94(1):37-47.e5. doi:

Insert

mEGFP-P2A-paAIP2

Use

AAV

Promoter

CaMKII 1.3

Available since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCS2 CA-LRP6-GFP

Plasmid

#29682

Depositor

Edward De Robertis

Publication

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LDL-receptor related protein 6 (LRP6 Human)

Tags

GFP

Expression

Mammalian

Mutation

extracellular region deleted

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pMV306G13+Lux

Plasmid

#26160

Depositor

Brian Robertson, Siouxsie Wiles

Publication

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

G13 promoter + Bacterial luciferase operon

Expression

Bacterial

Mutation

It contains a gram-positive enhanced translation …

Available since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Green-Camuialpha

Plasmid

#26933

Depositor

Ryohei Yasuda

Publication

Lee et al Nature. 2009 Mar 19. 458(7236):299-304.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

REACh-CaMKIIa-mEGFP

Tags

REACh and mEGFP

Expression

Mammalian

Available since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCMVTNT-T7-KPNA7

Plasmid

#26683

Depositor

Bryce Paschal

Publication

Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Karyopherin Alpha 7 (KPNA7 Human)

Tags

T7

Expression

Mammalian

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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p3869 HA-E6-AP C833A

Plasmid

#8649

Depositor

Peter Howley

Publication

Talis et al J Biol Chem 1998 Mar 13;273(11):6439-45.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

E6-AP (UBE3A Human)

Tags

HA

Expression

Mammalian

Mutation

C833A; active site Cys mutated to encode Ala

Available since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
Fuw-dCas9-dead Tet1CD-P2A-BFP

Plasmid

#108246

Purpose

Lentiviral vector to express dCas9-dead Tet1CD-P2A-tagBFP

Depositor

Rudolf Jaenisch

Publication

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

dCas9-dead Tet1CD-HA-P2A-BFP

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

dCas9-D10A, dCas9-H840A, Tet1CD-H1672Y, Tet1CD-D1…

Promoter

Ubc

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pGEX-2TK 14-3-3 sigma GST

Plasmid

#11944

Depositor

Michael Yaffe

Publication

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 sigma (SFN Human)

Tags

GST

Expression

Bacterial

Available since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pKS diaCas9_sgRNA

Plasmid

#74923

Purpose

Expresses Cas9 codon optimized for Phaeodactylum tricornutum and a sgRNA driven by a U6 promoter

Depositor

Per Winge

Publication

Nymark et al Sci Rep. 2016 Apr 25;6:24951. doi: 10.10

Inserts

LHCF2 promoter
diaCas9
LHCF1 terminator
U6 promoter
sgRNA
U6 3' region

Use

CRISPR

Promoter

Cas9 module and sgRNA module

Available since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pLVX-EGFP-C1-centrin-1

Plasmid

#73331

Purpose

Lentiviral vector encoding centrin-1 with an N-terminal EGFP tag

Depositor

Manuel Thery

Insert

centrin-1 (CETN1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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2PH-PLCdelta-GFP

Plasmid

#35142

Depositor

Sergio Grinstein

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

2PH-PLCdelta

Tags

EGFP

Expression

Mammalian

Mutation

Contains 2 tandem PH-PLCdelta domains

Promoter

CMV

Available since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pcDNA3.3_CoV2_501V2

Plasmid

#170449

Purpose

Express SARS-CoV-2 501V2 (South Africa strain / beta strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 501V2 pseudotyped virus production.

Depositor

David Nemazee

Publication

Yuan et al Science. 2021 Aug 13;373(6556):818-823.

Insert

SARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, 501V2 (B.1.351))

Tags

NA

Expression

Mammalian

Mutation

L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D6…

Promoter

CMV

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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