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pFA6a-mCherry-hphMX6

Plasmid

#105156

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

Insert

mCherry-hphMX6

Tags

mCherry

Expression

Bacterial and Yeast

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-mCherry-hphMX6

Plasmid

#105156

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

Insert

mCherry-hphMX6

Tags

mCherry

Expression

Bacterial and Yeast

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40

Plasmid

#101064

Purpose

AAV vector expressing CaMPARI2_L398T (Kd = 825nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Has Service

AAV1

Insert

CaMPARI2_L398T

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40

Plasmid

#101064

Purpose

AAV vector expressing CaMPARI2_L398T (Kd = 825nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Has Service

AAV1

Insert

CaMPARI2_L398T

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-SPOTlight-U2GCR

Plasmid

#164819

Purpose

Reporter to measure ISR state-dependent protein synthesis

Depositor

Nicole Calakos

Article

Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

Insert

SPOTlight-U2GCR

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-SPOTlight-U2GCR

Plasmid

#164819

Purpose

Reporter to measure ISR state-dependent protein synthesis

Depositor

Nicole Calakos

Article

Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

Insert

SPOTlight-U2GCR

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEFIRES-P-mCherry-HPos

Plasmid

#87159

Purpose

Fluorescent marker of lipid droplets (LDs)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

HPos

Tags

mCherry

Expression

Mammalian

Promoter

EF-1-alpha

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pEFIRES-P-mCherry-HPos

Plasmid

#87159

Purpose

Fluorescent marker of lipid droplets (LDs)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

HPos

Tags

mCherry

Expression

Mammalian

Promoter

EF-1-alpha

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH Yap S127A

Plasmid

#86497

Purpose

Mammalian expression of FLAG-tagged Yap containing S127A mutation

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Yes associated protein 1 (YAP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S127A

Promoter

EF1a

Available Since

Feb. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH Yap S127A

Plasmid

#86497

Purpose

Mammalian expression of FLAG-tagged Yap containing S127A mutation

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Yes associated protein 1 (YAP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S127A

Promoter

EF1a

Available Since

Feb. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pSMP-EZH2_1

Plasmid

#36387

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

EZH2 (EZH2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pSMP-EZH2_1

Plasmid

#36387

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

EZH2 (EZH2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc-NLRP3(R260W)

Plasmid

#73956

Purpose

Express c-Myc-tagged human NLRP3(R260W) in mammalian cells

Depositor

Christian Stehlik

Article

Dorfleutner et al Infect Immun. 2007 Mar;75(3):1484-92. Ep

Insert

NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)

Tags

c-myc

Expression

Mammalian

Mutation

R260W

Promoter

CMV iE

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-Myc-NLRP3(R260W)

Plasmid

#73956

Purpose

Express c-Myc-tagged human NLRP3(R260W) in mammalian cells

Depositor

Christian Stehlik

Article

Dorfleutner et al Infect Immun. 2007 Mar;75(3):1484-92. Ep

Insert

NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)

Tags

c-myc

Expression

Mammalian

Mutation

R260W

Promoter

CMV iE

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only
FCHo1-pmCherryC1

Plasmid

#27690

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

FCHo1 (Fcho1 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
FCHo1-pmCherryC1

Plasmid

#27690

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

FCHo1 (Fcho1 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
pEGFPC1-FLAG-Synaptojanin 1-170

Plasmid

#22294

Depositor

Pietro De Camilli

Article

Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 1

Insert

Synaptojanin 1_170 (SYNJ1 Human)

Tags

Flag and GFP

Expression

Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pEGFPC1-FLAG-Synaptojanin 1-170

Plasmid

#22294

Depositor

Pietro De Camilli

Article

Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 1

Insert

Synaptojanin 1_170 (SYNJ1 Human)

Tags

Flag and GFP

Expression

Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pTP288

Plasmid

#113460

Purpose

human Flag tagged Nbs1 in baculovirus transfer vector

Depositor

Tanya Paull

Article

Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

Insert

Nbs1 (NBN Human)

Tags

Flag

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTP288

Plasmid

#113460

Purpose

human Flag tagged Nbs1 in baculovirus transfer vector

Depositor

Tanya Paull

Article

Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

Insert

Nbs1 (NBN Human)

Tags

Flag

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge