We narrowed to 12,100 results for: VARS
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Plasmid#199238PurposeExpression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locusDepositorInsertS. pyogenes gRNA spacer
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterRNA polymerase III promoter for human U6 snRNAAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-2E2-HA-mEGFP
Plasmid#129595PurposeThe encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
ExpressionMammalianPromoterCMVAvailable SinceAug. 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-BE4max-NRTH
Plasmid#136919PurposeExpresses BE4max-NRTH in mammalian cellsDepositorInsertBE4max-NRTH
UseCRISPRExpressionMammalianMutationsee manuscriptPromoterCMVAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET23b-2E2-HA-mEGFP-6xHis
Plasmid#129594PurposeThe encoded protein is the anti-HA frankenbody variant fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.DepositorInsertAnti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
Tags6xHis-tagExpressionBacterialPromoterT7Available SinceOct. 28, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-2E2-HA-mCh
Plasmid#129596PurposeThe encoded protein is the anti-HA frankenbody variant fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody variant-mCherry (2E2-HA scFv-mCherry)
ExpressionMammalianPromoterCMVAvailable SinceAug. 27, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-ROS1-V5/HIS
Plasmid#205609PurposeExpression of human ROS1 receptor tyrosine kinase in mammalian cellsDepositorAvailable SinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-GFP-FLAG-cGAS
Plasmid#86675PurposeExpresses catalytically-active GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.DepositorInsertGFP-FLAG-cGAS
UseLentiviralAvailable SinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-tagRFP-FLAG-cGAS
Plasmid#86676PurposeExpresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.DepositorInserttagRFP-FLAG-cGAS
UseLentiviralAvailable SinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-L episome
Plasmid#169738PurposeEqualizer-L on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.DepositorInsertmiR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyExpressionMammalianPromoterpCMV-tetO2Available SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEMS2050
Plasmid#49135PurposeAAV plasmid with CAGGS promoter driving expression of EmGFP.DepositorInsertssAAV-CAGGS-emGFP
UseAAVExpressionMammalianPromoterCAGGSAvailable SinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPT234
Plasmid#127855PurposeRepressilator 2.0, without degradation tags, integrated triple reporterDepositorInsertsbla
mSCFP3
mKate2
mVenus
TetR
LacI
cI
UseSynthetic BiologyExpressionBacterialMutationFirst 11aa derived from mCherry as fusion proteinPromoterpAmpR, pL LacO1, pL tetO1, and pRAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
His-PIMT
Plasmid#34852DepositorAvailable SinceJan. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD)
Plasmid#188375PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
UseLentiviralAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES
Plasmid#200402PurposeEntry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detectionDepositorInsertClosed-loop DART VADAR sensor entry cassette
UseSynthetic BiologyExpressionMammalianAvailable SinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sniper2L
Plasmid#193856PurposeExpresses human codon-optimized Sniper2L and blasticidin resistance: EFS promoter-Sniper2L-NLS-FLAG-P2A-BSDDepositorInsertSniper2L
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationE1007LPromoterEFSAvailable SinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
HypaCas9
Plasmid#138557PurposeExpresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSDDepositorInsertHypaCas9
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationN692A, M694A, Q695A, H698APromoterEFSAvailable SinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2 ER:ras
Plasmid#67844Purpose4-hydroxytamoxifen inducible ER:ras fusion proteinDepositorInsertER1a H-RasG12V fusion
ExpressionMammalianMutationG525R renders the HBD largely insensitive to 17ß‐…PromoterCMVAvailable SinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
ABE8e SpG Puro
Plasmid#235044PurposeAdenosine base editor 8e* with SpG nicking Cas9 makes A > G editsDepositorInsertABE8e, nCas9
UseCRISPR and LentiviralTagsP2A-PuroRPromoterEF1a coreAvailable SinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-RRV
Plasmid#188543PurposeGateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editingDepositorInsertLbCas12a-RRV
UseCRISPR; Gateway compatible lbcas12a-rrv entry clo…TagsNLSExpressionPlantMutationG146R, D156R, R182VAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pY71-PT7-RiboJ-sfGFP-MGapt
Plasmid#129119PurposeExpresses a fluorescent reporter for the quantification of cell-free protein expression.DepositorInsertSuperfolder GFP with Malachite Green aptamer
UseSynthetic BiologyTagsHis6 and RiboJ InsulatorExpressionBacterialPromoterT7Available SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only