We narrowed to 11,632 results for: Vars

1. pCMV-BE4max-NRRH

   Plasmid

   #136918

   Purpose

   Expresses BE4max-NRRH in mammalian cells

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   BE4max-NRRH
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-AID-PcrA-UGI

   Plasmid

   #221220

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-PcrA-UGI
   

   Expression

   Mammalian

   Available Since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSP469

   Plasmid

   #65771

   Purpose

   Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135V, R1335Q, and T1337R mutations in Cas9

   Promoter

   CMV & T7

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-BE4max-NRTH

   Plasmid

   #136919

   Purpose

   Expresses BE4max-NRTH in mammalian cells

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   BE4max-NRTH
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3015b

   Plasmid

   #209317

   Purpose

   Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.

   Depositor

   Thomas Hooven

   Article

   Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1

   Insert

   Group B Streptococcus-compatible single guide RNA
   

   Expression

   Bacterial

   Promoter

   Xyl/tet promoter that is constitutively active

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcpBpaRS_v2

   Plasmid

   #196485

   Purpose

   To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.

   Depositor

   Kensaku Sakamoto

   Article

   Hino et al Methods Mol Biol. 2012;794:215-28. doi:

   Insert

   benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
   

   Expression

   Mammalian

   Promoter

   SV40(synthetase), Pol III tRNA promoter (tRNA), C…

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-ROR2-V5/HIS

   Plasmid

   #205608

   Purpose

   Expression of human ROR2 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   ROR2 receptor tyrosine kinase (ROR2 Human)
   

   Tags

   V5-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. CABE-T3.1

   Plasmid

   #193267

   Purpose

   Expression of CABE-T3.1 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CABE-T3.1
   

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHH0103_TP53BP2-1/1

   Plasmid

   #91503

   Purpose

   Protein expression and purification of human SH3 domain construct TP53BP2-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   TP53BP2-1/1 (TP53BP2 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS puro human-GFP-RLIM-IRES-mCherry

    Plasmid

    #232243

    Purpose

    Mammalian expression of RLIM-GFP-IRES-mCherry

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Insert

    RLIM-GFP-IRES-mCherry (RLIM Human)
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCFJ2451 - [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159845

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQE-BR1

    Plasmid

    #160333

    Purpose

    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.

    Depositor

    Sang Taek Jung

    Article

    Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12

    Insert

    BR1
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Equalizer-L episome

    Plasmid

    #169738

    Purpose

    Equalizer-L on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    pCMV-tetO2

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSP680

    Plasmid

    #65772

    Purpose

    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135E, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCPH2A.X-GS3

    Plasmid

    #204745

    Purpose

    Expression of Cas9 and human H2AX

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX (H2AX Human)
    

    Expression

    Mammalian

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-AID-Ns3h-UGI

    Plasmid

    #221219

    Purpose

    Express HACE Editor as a fusion of AID cytidine deaminase and Ns3h helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-Ns3h-UGI
    

    Expression

    Mammalian

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYDE009

    Plasmid

    #194151

    Purpose

    Delivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR.

    Depositor

    Edward Geisinger

    Article

    Bai et al J Bacteriol. 2021 May 20;203(12):e005652

    Insert

    tetR-tetP-dcas9-rrnB T1-T7Te
    

    Expression

    Bacterial

    Promoter

    tetp

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-mRIN2

    Plasmid

    #108940

    Purpose

    Mammalian expression of mRIN2

    Depositor

    Guido Serini

    Article

    Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

    Insert

    Ras and Rab interactor 2 (Rin2 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAcGFP1-N1-RvCAHS3-GS

    Plasmid

    #192460

    Purpose

    Expression of RvCAHS3-GS_linker-AcGFP1 in mammalian cells

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS3
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEY43

    Plasmid

    #191047

    Purpose

    eat-4(prom6-1) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    eat-4(prom6-1) (eat-4 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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