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Murine cell surface protein knockout (McspKO) library

Pooled library

#200445

Purpose

Knockout library targeting mouse cell surface genes.

Depositor

Junjie Chen

Publication

Wang et al Proc Natl Acad Sci U S A. 2022 Jun 21;11

Expression

Mammalian

Species

Mus musculus

Use

CRISPR, Lentiviral, and Mouse Targeting

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only
pTS117 His14-Avi-45xGS-anti GFP nanobody

Plasmid

#199370

Purpose

Bacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

Depositor

Rebecca Voorhees

Publication

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-GFP nanobody

Tags

14xHis-Avi

Expression

Bacterial

Mutation

WT

Promoter

T5-LacO

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTS117 His14-Avi-45xGS-anti GFP nanobody

Plasmid

#199370

Purpose

Bacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

Depositor

Rebecca Voorhees

Publication

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-GFP nanobody

Tags

14xHis-Avi

Expression

Bacterial

Mutation

WT

Promoter

T5-LacO

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1 SARS-CoV-2 S D614G

Plasmid

#158075

Purpose

Expresses SARS-CoV-2 spike protein with a D614G mutation

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 spike (S )

Expression

Mammalian

Mutation

D614G

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pcDNA3.1 SARS-CoV-2 S D614G

Plasmid

#158075

Purpose

Expresses SARS-CoV-2 spike protein with a D614G mutation

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 spike (S )

Expression

Mammalian

Mutation

D614G

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pCXN2-HA-AT1R-YFP

Plasmid

#101659

Purpose

Expresses AT1R with HA tag and YFP in mammalian cells.

Depositor

Yusuke Ohba

Publication

Inuzuka et al Sci Rep. 2016 Feb 9;6:21613. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Angiotensin II type 1 receptor (AGTR1 Human)

Tags

HA and YFP (Venus)

Expression

Mammalian

Promoter

CAG promoter

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCXN2-HA-AT1R-YFP

Plasmid

#101659

Purpose

Expresses AT1R with HA tag and YFP in mammalian cells.

Depositor

Yusuke Ohba

Publication

Inuzuka et al Sci Rep. 2016 Feb 9;6:21613. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Angiotensin II type 1 receptor (AGTR1 Human)

Tags

HA and YFP (Venus)

Expression

Mammalian

Promoter

CAG promoter

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pEGFP-sfCherry11-ß-actin

Plasmid

#70221

Purpose

Expresses sfCherry11-ß-actin in mammalian cells

Depositor

Bo Huang

Publication

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sfCherry11-actin (ACTB Human)

Tags

sfCherry11

Expression

Mammalian

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-sfCherry11-ß-actin

Plasmid

#70221

Purpose

Expresses sfCherry11-ß-actin in mammalian cells

Depositor

Bo Huang

Publication

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sfCherry11-actin (ACTB Human)

Tags

sfCherry11

Expression

Mammalian

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUB1 (full-length, aa 1-271)

Plasmid

#61420

Purpose

Expresses human OTUB1 (full-length) in E. coli.

Depositor

David Komander

Publication

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUB1 (Ubiquitin thioesterase OTUB1) (OTUB1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pOPINK-OTUB1 (full-length, aa 1-271)

Plasmid

#61420

Purpose

Expresses human OTUB1 (full-length) in E. coli.

Depositor

David Komander

Publication

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUB1 (Ubiquitin thioesterase OTUB1) (OTUB1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAdEasy mCherry-Tubulin

Plasmid

#26767

Depositor

Torsten Wittmann

Publication

Matov et al Nat Methods. 2010 Sep . 7(9):761-8.

Insert

Tubulin (TUBA1B Human)

Use

Adenoviral

Tags

mCherry

Expression

Mammalian

Available since

April 8, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAdEasy mCherry-Tubulin

Plasmid

#26767

Depositor

Torsten Wittmann

Publication

Matov et al Nat Methods. 2010 Sep . 7(9):761-8.

Insert

Tubulin (TUBA1B Human)

Use

Adenoviral

Tags

mCherry

Expression

Mammalian

Available since

April 8, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pBabe.puro.CDK8.flag

Plasmid

#19758

Depositor

William Hahn

Publication

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

cyclin-dependent kinase 8 (CDK8 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

Dec. 11, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBabe.puro.CDK8.flag

Plasmid

#19758

Depositor

William Hahn

Publication

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

cyclin-dependent kinase 8 (CDK8 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

Dec. 11, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pmr101A-hVPS26

Plasmid

#17636

Depositor

James Hurley

Publication

Shi et al Nat Struct Mol Biol. 2006 Jun . 13(6):54

Insert

VPS26 (VPS26A Human)

Tags

His

Expression

Bacterial

Available since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pmr101A-hVPS26

Plasmid

#17636

Depositor

James Hurley

Publication

Shi et al Nat Struct Mol Biol. 2006 Jun . 13(6):54

Insert

VPS26 (VPS26A Human)

Tags

His

Expression

Bacterial

Available since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pRetroX-PTuner DD-linker-M.EcoGII

Plasmid

#122082

Purpose

Express M.EcoGII with detabilization domain - inducible

Depositor

Laure Crabbe

Publication

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII

Use

Retroviral

Tags

V5 tag on Nter of M.EcoGII

Expression

Bacterial and Mammalian

Available since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pRetroX-PTuner DD-linker-M.EcoGII

Plasmid

#122082

Purpose

Express M.EcoGII with detabilization domain - inducible

Depositor

Laure Crabbe

Publication

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII

Use

Retroviral

Tags

V5 tag on Nter of M.EcoGII

Expression

Bacterial and Mammalian

Available since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCas9-HE

Plasmid

#109400

Purpose

Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein

Depositor

Jean-Paul Concordet

Publication

Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HDR-Enhancer domain of human CtIP protein (RBBP8 Human)

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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