We narrowed to 6,787 results for: PROC
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Plasmid#127165PurposeVector for integrating the process (pGAL1 expression of Z3PM and mKate2 both fused to psd) and the circuit output (pZ3-Venus-cODC)DepositorInsertpGAL1-Z3PM-psd-tADH1-pGAL1-mKate2-psd-tPGK1-pZ3-Venus-cODC-tTDH1
UseSynthetic BiologyAvailable SinceAug. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pp-CBBC-Fluc
Plasmid#232920PurposeAllows the Golden Gate (BsmBI) cloning of pegRNA between two Csy4-hairpins in the intron of a CMV-FLUC encoding plasmid (POL II): ipegRNAs must be processed by Csy4 in VLP- producer cells.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterCMVAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28Man
Plasmid#203399Purposeexo-beta mannosidase useful in enzymatic biotransformations of glycans and in coupled assays of various carbohydrate processing enzymesDepositorInsertman2A
TagsH6ExpressionBacterialMutationNonePromoterT7Available SinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsRRP12-EBMmut_K
Plasmid#146767PurposeMammalian Expression of HsRRP12-EBMmutDepositorInsertHsRRP12-EBMmut (RRP12 Human)
ExpressionMammalianAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.0
Plasmid#190795PurposePRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements. Step 1 in cloning processDepositorInsertPRE-Hsp70Bb-Cas9-T2A-eGFP
UseCRISPRExpressionInsectAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTC366
Plasmid#91215Purposeprotoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, ribozyme processing)DepositorInsert2 gRNAs targeting tomato ARF8A
UseCRISPRExpressionPlantAvailable SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
gRNA-CasRx_SD1-pSV40-TagBFP
Plasmid#221004PurposeTransiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.DepositorInsertCasRx 30nt processed direct repeat with SD1 mutation
UseCRISPRExpressionMammalianMutationDR1 A>TPromoterU6Available SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Lb
Plasmid#86197PurposeLbCpf1 Gateway gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processingDepositorInsertgRNA cloning site
UseCRISPRPromoterMaize ubiquitin 1Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
PGK1p-Csy4-pA (Construct 2)
Plasmid#55196PurposeExpresses Csy4 in Mammalian cells for site-specific RNA processingDepositorInsertCsy4/Cas6
UseSynthetic BiologyTags6xHISExpressionMammalianPromoterCMVAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-As
Plasmid#86196PurposeAsCpf1 gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processingDepositorInsertAsCpf1 gRNA cloning site for ribozyme cleavage
UseCRISPRTagsHammerhead ribozyme and HDV ribozymeExpressionPlantPromoterMaize ubiquitin 1Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 CARD8-TEV-GFP-FLAG S297A
Plasmid#169992PurposeMammalian purification construct for CARD8 with an autoprocessing-deficient mutationDepositorAvailable SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
FZ-NbPDS
Plasmid#184268PurposepAIDE containing a full-length CPSMV RNA2 cDNA modified to accomodate an NbPDS fragment.DepositorInsertCPSMV RNA2 cDNA, modified to permit foreign gene insertion between MP and L-CP
ExpressionPlantMutationThe protease processing site between MP and L-CP …PromoterCaMV 35S with duplicated enhancerAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-SHMY-PAUF
Plasmid#182645PurposeMammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and PAUF sequenceDepositorInsertPancreatic adenocarcinoma upregulated factor (PAUF)
UseRetroviralTagsHis6-tag, N-terminal ER targeting sequence of hem…ExpressionMammalianPromoterCMVAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IBU-pSu9-HaloTag7-SNAP
Plasmid#217319PurposeExpresses the presequence of Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag for measuring mitophagy activity by Halo processing assay.DepositorInsertNeurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag
UseRetroviralTagsHaloTag and SNAPPromoterCMVAvailable SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Fn
Plasmid#108864PurposeFnCpf1 Gateway crRNA entry plasmid using Zea mays Ubi promoter and double ribozyme processingDepositorTypeEmpty backboneUseCRISPR; Gateway compatible crrna entry cloneExpressionPlantAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.2
Plasmid#190796PurposegypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.DepositorInsertgypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
ExpressionInsectAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-3xTS
Plasmid#162777PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to three tyrosine sulfation (TS) motifs. LaM4-3xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to three TS sites, T7, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialPromoterT7Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
HEpl017
Plasmid#179992PurposepCBh_NLS_TadA8e_dSiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized dSiCas12i-ABE8e driven by CBh promoter and tagmCherry driven by CMV promoterDepositorInsertTadA8e-dSiCas12i(D1049A)
ExpressionMammalianAvailable SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8-FLAG S297A
Plasmid#169949PurposeMammalian expression of CARD8-FLAG that is autoprocessing-deficient (S297A)DepositorAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS ColIII alpha1
Plasmid#19187DepositorInsertProcollagen type III alpha 1 (COL3A1 Chicken)
ExpressionBacterialAvailable SinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 EBNA1BP2
Plasmid#67072PurposeStandard for cell-free expression benchmarking.DepositorInsertEBNA1BP2 EBNA1BP2 EBNA1 binding protein 2 (EBNA1BP2 Human)
UseCell-free expressionTagsEGFP and HisPromoterT7Available SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-1xTS
Plasmid#162778PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to a TS site, T7, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialPromoterT7Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZJQIBEBT003
Plasmid#140053PurposeThe pZJQIBEBT003 plasmid contains the coding sequence of eMutS-L157C-G233C gene which has the ability of error removal in the gene synthesis process.DepositorInserteMutS-L157C-G233C-CBM-EGFP
TagsHis-TagExpressionBacterialMutationL157C, G233CPromoterT7Available SinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-SMO-1(GG)
Plasmid#87054PurposeExpresses 6xHis-tagged smo-1(GG), with a TEV protease cleavage site between the tag and the ORFDepositorInsertsmo-1 (smo-1 Nematode)
Tags6xHis-TEVExpressionBacterialMutationThe last aa was removed to render processed SMO-1…PromoterT7Available SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUBI-RZ-Cas12j2
Plasmid#189781PurposeCas12j2 (CasΦ) Gateway gRNA entry plasmid using Zea mays Ubi promoter and HH, HDV ribozyme processingDepositorInsertgRNA cloning site
UseCRISPRAvailable SinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-2xTS
Plasmid#162779PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to two tyrosine sulfation (TS) motifs. LaM4-2xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to two TS sites, T7, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialPromoterT7Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HLA-cmyc-GRM3 C127A TMD Y616M
Plasmid#140454PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and Y616M mutantDepositorInsertGRM3 (GRM3 Human)
TagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, tyrosine 616 to methioni…Available SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HLA-cmyc-GRM3 C127A TMD L624N
Plasmid#140455PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and L624N mutantDepositorInsertGRM3 (GRM3 Human)
TagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, leucine 624 to asparagineAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HLA-cmyc-GRM3 C127A TMD A634N
Plasmid#140456PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and A634N mutantDepositorInsertGRM3 (GRM3 Human)
TagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, alanine 634 to asparagineAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4_HLA-flag-GRM3 C127A-VN
Plasmid#140449PurposeMammalian expression plasmid for FLAG-tagged mGluR3 fused to the N-terminal half of split fluorescent Venus (VN)DepositorInsertGRM3 (GRM3 Human)
TagsFLAG tag, Signal/leader sequence from HLA class I…ExpressionMammalianMutationCysteine 127 to alanineAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only