We narrowed to 4,836 results for: HRE
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Plasmid#91812PurposeYeast expression of S. cerevisiae Rpb1 with a T1471A mutationDepositorInsertRPO21 (RPO21 Budding Yeast)
ExpressionYeastMutationchanged threonine 1471 to alaninePromoterEndogenous Rpb1 promoterAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-CD8a(7A)
Plasmid#162498PurposeExpresses GFP tagged CD8a mutant in mammalian cells. The seven O-glycosylation sites in its stem region are mutated.DepositorInsertCD8a with a mutated stem region (CD8A Human)
TagsGFPExpressionMammalianMutationThe five threonine and two serine residues are mu…Available SinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Met133I, M1381I, M186I ELL2
Plasmid#127263PurposeFor in vitro translation of human ELL2 with Met133I, M1381I, M186IDepositorInsertELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
UseIn vitro translationMutationThree Mets (133, 138, 186) to IleuPromoterT7Available SinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONRG_P4-P1R:TBA2p
Plasmid#128429PurposeGateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing the TBA2 (At1g62060) promoter sequence (1346 base pairs), for use in Three-way Gateway cloning.DepositorInsertTESTA ABUNDANT 2 (AT1G62060 Mustard Weed)
UseGateway promoter entry clonePromoterTESTA ABUNDANT 2Available SinceOct. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19_T790M_28bp
Plasmid#114721Purpose28 bp fragment of the EGFR T790M allele used as SiMREPS analyte standardDepositorInsertEGFR (EGFR Human)
ExpressionBacterialMutationc.2369C>T (Substitution, position 2369, C➞T); …Available SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19_4xT790M_28bp
Plasmid#114719Purpose4 x 28 bp repeats of the EGFR T790M allele used as SiMREPS analyte standardDepositorInsertEGFR (EGFR Human)
ExpressionBacterialMutationc.2369C>T (Substitution, position 2369, C➞T); …Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
csd2_121-308,T151A/pET28b(+)
Plasmid#83300Purposecsd2, construct: 121-308, pET28b(+) NC-tag, mutation: T151ADepositorInserthp1544
TagsHis tagExpressionBacterialMutationdeleted amino acid 1-120, and changed Threonine 1…Available SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hERG1a S620T
Plasmid#53059PurposeExpresses alpha subunit of S620T hERG1a K channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseXenopus oocyte expressionMutationchanged Serine 620 to ThreoninePromoterSP6Available SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEBG MKK3b(Glu, delete LXL)
Plasmid#50452Purposeactivates p38 in the signaling pathwayDepositorInsertMKK3b (MAP2K3 Human)
TagsGstExpressionMammalianMutationdeleted L25-I27, changed Serine 218 to Glutamic A…PromoterSV40Available SinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT-7th R
Plasmid#42573DepositorInsertmutated calmodulin gene
TagsPolyhistidine (6×His) region, TEV recognition sit…ExpressionBacterialMutationchanged Phenylalanine 92 to Glutamine acid, Isole…PromoterT7Available SinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
p5E-elavl3
Plasmid#75025PurposeCan be used to drive pan neuronal expression in zebrafish.DepositorInsertELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
Use5' entry vector for multisite gateway three …Promoterelavl3Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_T221E
Plasmid#108108PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TE mutation at LKB1 phosphorylation site) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF2582
Plasmid#142436PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2198
Plasmid#142924PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDONR P4-P1R-EGFP
Plasmid#48348PurposeContributes the coding sequence for EGFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertEGFP
UseGateway entry vectorMutationContains a Kozak sequence. Does not contain a sto…PromoternoneAvailable SinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-AID-BubR1
Plasmid#47330Purposefor plasmid integration using the Flp-In system. AID at N-terminalDepositorInsertBUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
TagsAID and GFPExpressionMammalianMutationsiRNA resistantAvailable SinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEBG-MKK4-S255D/T259E (GST)
Plasmid#21566DepositorInsertmitogen-activated protein kinase kinase 4 (Map2k4 Mouse)
TagsGSTExpressionMammalianMutationSerine 255 to Aspartic Acid, Threonine 259 to Gl…Available SinceJuly 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant))
Plasmid#218508Purposeintegrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integraseDepositorInsertmNeongreen
Tagsmodified ssrA degradation tagExpressionBacterialMutationdeletion of MCS containing Plac_lacZa, introducti…PromoterP23Available SinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS
Plasmid#154774Purposeplasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma PylRS for ochre suppression in transient or stable, piggybac-mediated, integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
TagsFLAGExpressionMammalianMutationin PylT: UUA anticodon and G10A, G14U, G16U, C20U…PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF2195
Plasmid#142181PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only