We narrowed to 48,883 results for: des.1

1. pLenti X2 Neo/pTER shEGFP#1 (w21-2)

   Plasmid

   #17479

   Purpose

   3rd gen lentiviral eGFP shRNA #1 (H1/TO promoter), 3’LTR insertion, Neo 

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   Enhanced green fluorescent protein shRNA #1
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GLN, Linear-3, PolyU(1), Hairpin Distance = 0nt

   Plasmid

   #166986

   Purpose

   Tests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination module:PolyU(1), Hairpin = 0 nt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GLY, Linear-3, PolyU(1), Hairpin Distance = 0nt

   Plasmid

   #166990

   Purpose

   Tests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination module:PolyU(1), Hairpin = 0 nt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)

   Plasmid

   #52898

   Purpose

   YpdI* is fused to the IFP-F[2] by (GGGGS) X 2. Ypd1*/Skn7t: no signal, Ypd1*/Ssk1t: weak signal. Internal ClaI site in Ypd*I. Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   Ypd*1-L-IFP-F[2]
   

   Use

   Tags

   L-IFP-F[2]

   Expression

   Yeast

   Mutation

   Changed Tryptophane 80 to Alanine

   Promoter

   GAL1

   Available since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)

   Plasmid

   #196703

   Purpose

   Plasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

   Insert

   human RNaseH1 D210N
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D210N, catalytically inactive

   Promoter

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)

   Plasmid

   #101297

   Purpose

   FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

   Depositor

   Alpha Yap

   Article

   Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

   Insert

   alpha-Catenin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains amino acids 1-906 of alpha-Catenin

   Promoter

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-1)

   Plasmid

   #159659

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. aCat TL2 (1-697 mTFP1-TSmod-Venus STOP)

   Plasmid

   #101298

   Purpose

   FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

   Depositor

   Alpha Yap

   Article

   Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

   Insert

   alpha-Catenin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains amino acids 1-698 of alpha-Catenin

   Promoter

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p6346 MSCV-CMV-Flag-HA-Brd4-1-444

   Plasmid

   #32886

   Depositor

   Peter Howley

   Article

   Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

   Insert

   Brd4 (BRD4 Human)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p5064 pcDNA3.1/V5-His-TOPO hBrd4 (1-461)

    Plasmid

    #14443

    Depositor

    Peter Howley

    Article

    You et al Cell. 2004 Apr 30. 117(3):349-60.

    Insert

    Brd4 (1-461) (BRD4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    amino acids 1-461

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pBABE Gal4 (1-94) HSF ABC Flag wt

    Plasmid

    #1945

    Depositor

    Robert Kingston

    Article

    Sullivan et al Mol Cell Biol 2001 Sep;21(17):5826-37.

    Insert

    Heat Shock Factor 1 (HSF1 Human)
    

    Use

    Retroviral

    Tags

    Gal4 DBD and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pBABE Gal4(1-94) HSF ABC Flag ph

    Plasmid

    #1947

    Depositor

    Robert Kingston

    Article

    Sullivan et al Mol Cell Biol 2001 Sep;21(17):5826-37.

    Insert

    Heat Shock Factor 1 (HSF1 Human)
    

    Use

    Retroviral

    Tags

    Gal4 DBD and flag

    Expression

    Mammalian

    Mutation

    phenylalanine mutant (F418, F492, F500 A)

    Promoter

    Available since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pAc5.1-PH-mCherry-human p53 1-50 (HK180)

    Plasmid

    #206458

    Purpose

    p53 1-50 expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Insert

    p53 1-50 (TP53 Human)
    

    Use

    Tags

    PH mcherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. YIp211-ADH-myc-E3(1)-CUbo-mCherry-VSV

    Plasmid

    #212740

    Purpose

    Consitutive expression of myc-E3(1)-CUbo-mCherry-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(1)
    

    Use

    Integrative vector

    Tags

    CUbo-mCherry-VSV and myc

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. YIp128-ADH-myc-E3(1)-CUbo-mCherry-VSV

    Plasmid

    #212739

    Purpose

    Consitutive expression of myc-E3(1)-CUbo-mCherry-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(1)
    

    Use

    Integrative vector

    Tags

    CUbo-mCherry-VSV and myc

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. YIp128-ADH-myc-E3(1)-CUbo-NLS-VSV

    Plasmid

    #212737

    Purpose

    Consitutive expression of myc-E3(1)-CUbo-NLS-VSV in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(1)
    

    Use

    Integrative vector

    Tags

    CUbo-NLS-VSV and myc

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-6P-1-HsTNRC6Biso1_1361-1723-del1395-1534-6His_N

    Plasmid

    #147026

    Purpose

    Bacterial Expression of HsTNRC6Biso1_1361-1723-del1395-1534

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

    Insert

    HsTNRC6Biso1_1361-1723-del1395-1534 (TNRC6B Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-6P-1 Drosophila melanogaster CBS-pair domain

    Plasmid

    #166436

    Purpose

    Express pGEX-6P-1 Drosophila melanogaster CBS-pair domain

    Depositor

    Kalle Gehring

    Article

    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

    Insert

    Drosophila melanogaster CBS-pair domain (Cbs Fly)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac, LacI, Amp

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-1)

    Plasmid

    #159662

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. psiCHECK-2-Ywhab-3'UTR-MRE-1 MUT

    Plasmid

    #61796

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Ywhab with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Ywhab 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only