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pHR-DARPin-P-BFP

Plasmid

#246865

Purpose

Express DARPin-P-BFP for RSV imaging.

Depositor

Marvin Tanenbaum

Publication

Ratnayake et al Nature. 2026 Jan 28. doi: 10.1038/s41586

Insert

DARPin-P (clone ID_011-1055-C6-2605-H5)

Use

Lentiviral

Tags

BFP

Expression

Mammalian

Promoter

SFFV

Available since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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PAC_E83_LOV_eGFP

Plasmid

#231574

Purpose

This plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue light

Depositor

Dominik Niopek

Publication

Wolf et al Nat Methods. 2025 Aug;22(8):1698-1706. d

Insert

PAC-P2A-GFP carrying an insertion of AsLOV2 behind E83

Expression

Mammalian

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLBS.EF/miRFP670-hGem110Nt

Plasmid

#129333

Purpose

Cell cycle reporter, lentivirus

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

miRFP670 (Addgene 80006)

Use

Lentiviral

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAW-AlphaLobe

Plasmid

#113255

Purpose

E. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

Depositor

Jennifer Doudna

Publication

Wright et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

S. pyogenes Cas9 alpha-helical lobe

Tags

10xHis-MBP

Expression

Bacterial

Mutation

residues 56–714; See paper for description

Promoter

T7

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pPK1E-1+t-gfp

Plasmid

#48131

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Publication

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Expression

Bacterial

Available since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSADuet760

Plasmid

#45613

Depositor

Hideo Iwai

Publication

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

MjaTFIIB mini intein

Tags

GB1 and H6-GB1

Expression

Bacterial

Promoter

T7

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pSABAD250

Plasmid

#45612

Depositor

Hideo Iwai

Publication

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

NpuDnaBC39

Tags

GB1-H6

Expression

Bacterial

Promoter

pBAD

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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MST4

Plasmid

#39172

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

MST4 (STK26 Human)

Tags

His Tev

Expression

Bacterial

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pCS2-Flag-Kdrl-5p-FokI-EL

Plasmid

#35684

Depositor

Scot Wolfe

Publication

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

Kdrl-5p-FokI-EL

Use

Mrna production

Tags

FLAG and Flag

Available since

April 10, 2012

Availability

Academic Institutions and Nonprofits only
pLPP6

Plasmid

#209968

Purpose

Contains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertion

Depositor

Thorsten Mascher

Publication

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter Dropout (sfGFP)

Expression

Bacterial

Available since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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mCherry-C1-ORP5deltaORDdeltaTMD-FKBP

Plasmid

#220074

Purpose

expresses ORP5 with the OSBP-related ligand-binding domain and the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.

Depositor

Gerry Hammond

Publication

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

OSBPL5deltaORDdeltaTMD (OSBPL5 Human)

Expression

Mammalian

Mutation

deltaORDdeltaTMD

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-ChREBPα

Plasmid

#211754

Purpose

Cloning vector for the expression of mouse ChREBPα

Depositor

Russell Goodman

Publication

Singh et al Cell Metab. 2024 Jan 2;36(1):144-158.e7.

Insert

MLXIPL (Mlxipl Mouse)

Use

Cloning vector

Available since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGL2_171

Plasmid

#199103

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pWKS1 ORI plus NGS barcode 17, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_169

Plasmid

#199101

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pIJ101 ORI plus NGS barcode 15, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_173

Plasmid

#199105

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBBR1-UP ORI plus NGS barcode 21, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPPC017.109

Plasmid

#171145

Purpose

Expression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-KmR plasmid

Depositor

Jesse Zalatan

Publication

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

J109 scRNA
J1-BBa_J23117-mRFP

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 and J1-BBa_J23117

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMOD3-mClover-nat

Plasmid

#120328

Purpose

Bacterial mClover promoter trap

Depositor

James Kirby

Publication

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mClover

Expression

Bacterial

Mutation

D103N (see comments below)

Promoter

(None)

Available since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pSLBPres

Plasmid

#82454

Purpose

Encodes synthesized SLBP that is resistant to SMARTpool siRNA

Depositor

Bharat Ramratnam

Publication

Li et al J Clin Invest. 2016 Jul 25. pii: 82360.

Insert

SLBP (SLBP Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only
pT7ddx19gRNA

Plasmid

#47932

Purpose

in vitro transcription of ddx19 gRNA

Depositor

Wenbiao Chen, Susan Wente

Publication

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

Insert

ddx19 gRNA (ddx19 Zebrafish)

Use

CRISPR

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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GMDS

Plasmid

#39152

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

GMDS (GMDS Human)

Tags

His Tev

Expression

Bacterial

Mutation

codon-optimized

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only