We narrowed to 8,355 results for: construct

1. pUC19-J9E534

   Plasmid

   #103078

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   J9E534(Cas9 coding gene from Alicyclobacillus hesperidum URH17-3-68)
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUC19-K2FTY8

   Plasmid

   #103079

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   K2FTY8(Cas9 coding gene from Alcanivorax pacificus W11-5)
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC19-A8LN05

   Plasmid

   #103083

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   A8LN05(Cas9 coding gene from Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12))
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC19-B1UZL4

   Plasmid

   #103085

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   B1UZL4(Cas9 coding gene from Clostridium perfringens D str. JGS1721)
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUC19-B8I085

   Plasmid

   #103089

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   B8I085(Cas9 coding gene from Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10))
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUC19-Q5L8F8

   Plasmid

   #103126

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   Q5L8F8(Cas9 coding gene from Bacteroides fragilis (strain ATCC 25285 / NCTC 9343))
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-SARS-CoV-2-S-ectodomain

   Plasmid

   #235984

   Purpose

   Internally tagged SARS-CoV-2 spike ecto-domain

   Depositor

   Syed Saif Hasan

   Article

   Singh et al J Struct Biol X. 2025 Feb 11;11:100123.

   Insert

   Spike protein (S SARS-CoV-2)
   

   Use

   Tags

   8xHis and Twin-Strep tag and internal Twin-Strep …

   Expression

   Mammalian

   Mutation

   aa 1-1208, D614G, RRAR684-684GSAS, and DK987-987P…

   Promoter

   CMV

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. GAL4-Creb5 (1- 128) T59/T61 A

   Plasmid

   #195032

   Purpose

   GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with T59/T61 A mutation)

   Depositor

   Cheng-Hai Zhang, Andrew Lassar

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with with T59/T61 A mutation) (CREB5 Bovine)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T59/T61 A

   Promoter

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP Cav2.2 D122A pcDNA3

   Plasmid

   #206068

   Purpose

   expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a D122A mutation that disrupts the interaction with ?2?-1

   Depositor

   Annette Dolphin

   Article

   Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. KTK_219

    Plasmid

    #180534

    Purpose

    Entry vector containing K.rhaeticus native promoter pCmcAX (bcs1) (200 bases upstream from gene start codon)

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    K.rhaeticus native promoter pCmcAX (bcs1) (200 bases upstream from gene start codon)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. KTK_223

    Plasmid

    #180538

    Purpose

    Entry vector containing K.rhaeticus native promoter pHxu (200 bases upstream from gene start codon)

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    K.rhaeticus native promoter pHxu (200 bases upstream from gene start codon)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. KTK_226

    Plasmid

    #180552

    Purpose

    Entry vector containing K.rhaeticus native promoter pRutB (200 bases upstream from gene start codon)

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    K.rhaeticus native promoter pRutB (200 bases upstream from gene start codon)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. KTK_218

    Plasmid

    #180533

    Purpose

    Entry vector containing K.rhaeticus native promoter pTtaC (200 bases upstream from gene start codon)

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    K.rhaeticus native promoter pTtaC (200 bases upstream from gene start codon)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KTK_225

    Plasmid

    #180539

    Purpose

    Entry vector containing K.rhaeticus native promoter pBlh (200 bases upstream from gene start codon)

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    K.rhaeticus native promoter pBlh (200 bases upstream from gene start codon)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KTK_221

    Plasmid

    #180536

    Purpose

    Entry vector containing K.rhaeticus native promoter pKr0953 (200 bases upstream from gene start codon)

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    K.rhaeticus native promoter pKr0953 (200 bases upstream from gene start codon)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. KTK_220

    Plasmid

    #180535

    Purpose

    Entry vector containing K.rhaeticus native promoter pAacsAB4 (200 bases upstream from gene start codon)

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    K.rhaeticus native promoter pAacsAB4 (200 bases upstream from gene start codon)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. KTK_222

    Plasmid

    #180537

    Purpose

    Entry vector containing K.rhaeticus native promoter pCyd (200 bases upstream from gene start codon)

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    K.rhaeticus native promoter pCyd (200 bases upstream from gene start codon)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ARB366

    Plasmid

    #124049

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::KRAB-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ARB367

    Plasmid

    #124050

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC19-B2KB46

    Plasmid

    #103086

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    B2KB46(Cas9 coding gene from Elusimicrobium minutum (strain Pei191))
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge