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pAC-U61-SapI

Plasmid

#112808

Purpose

gRNA cloning vector for expressing 1-3 gRNAs in Drosophila

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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KG#244

Plasmid

#110927

Purpose

Vector for expressing proteins in a subset of 9 DA/ DB cholingergic motor neurons in the C. elegans ventral nerve cord

Depositor

Kenneth Miller

Article

Edwards et al J Cell Biol. 2009 Sep 21;186(6):881-95.

Inserts

unc-129 promoter
unc-54 3' control region

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1178 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 dCTD EcoRV)

Plasmid

#107860

Purpose

To quickly insert CTD mutants into TDP43 splicing reporter

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

Deletion of CTD in TDP43 splicing reporter, inser…

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only
pJExpress414_GmPOPB-R663K

Plasmid

#92239

Purpose

Express R663K mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-R663K mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Arginine 663 to Lysine

Promoter

T7 promoter

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-W695del

Plasmid

#92241

Purpose

Express W695-deletion mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-W695deletion mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Tryptophan 695 deleted

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-R663Q

Plasmid

#92240

Purpose

Express R663Q mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-R663Q mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Arginine 663 to Glutamine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-H698A

Plasmid

#92237

Purpose

Express H698A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-H698A mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Histidine 698 to Alanine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-R663A

Plasmid

#92238

Purpose

Express R663A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-R663A mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Arginine 663 to Alanine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRS413 Prp5 E235A

Plasmid

#87601

Purpose

Encodes Prp5 E235A for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

E235A

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRS413 Prp5 N399D

Plasmid

#87602

Purpose

Encodes Prp5 N399D for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

N399D

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-PRP2

Plasmid

#87591

Purpose

Encodes Y2H Gal4 DNA binding domain for Prp2

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP2 (PRP2 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 P369E

Plasmid

#87277

Purpose

Encodes Hsh155 P369E for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

P369E

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 K335E

Plasmid

#87273

Purpose

Encodes Hsh155 K335E for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

K335E

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 R294L

Plasmid

#87269

Purpose

Encodes Hsh155 R294L for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

R294L

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 R294C

Plasmid

#87268

Purpose

Encodes Hsh155 R294C for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

R294C

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 Y292C

Plasmid

#87267

Purpose

Encodes Hsh155 Y292C for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Y292C

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-YSF3

Plasmid

#87598

Purpose

Encodes Y2H Gal4 DNA binding domain for Ysf3

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

YSF3 (YSF3 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-BUD13

Plasmid

#87547

Purpose

Encodes Y2H Gal4 DNA binding domain for Bud13

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

BUD13 (BUD13 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-SLU7

Plasmid

#87597

Purpose

Encodes Y2H Gal4 DNA binding domain for Slu7

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

SLU7 (SLU7 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-PRP28

Plasmid

#87595

Purpose

Encodes Y2H Gal4 DNA binding domain for Prp28

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP28 (PRP28 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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