We narrowed to 80,332 results for: TRI

1. pRAN473

   Plasmid

   #120813

   Purpose

   E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein

   Depositor

   David S. Weiss, Craig Ellermeier

   Article

   Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

   Insert

   red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pc3.1-CAGGS-ASAP3-mCyRFP3

   Plasmid

   #193323

   Purpose

   A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3

   Depositor

   Michael Lin

   Article

   Kim et al Sci Rep. 2022 Mar 7;12(1):3678. doi: 10.

   Insert

   ASAP3-R3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   caggs

   Available since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

3. gRNA-ura-HYB

   Plasmid

   #64330

   Purpose

   ura3 deletion gRNA cassette carried by pRS42H

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   gBlock product of ura3 deletion gRNA cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

4. T7-VEE-GFP

   Plasmid

   #58977

   Purpose

   expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Insert

   EGFP
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   26S subgenomic promoter

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. LAMP1-mGFP

   Plasmid

   #34831

   Depositor

   Esteban Dell'Angelica

   Article

   Falcon-Perez et al J Cell Sci. 2005 Nov 15;118(Pt 22):5243-

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Tags

   GSTGSTGSTGA linker, Kozak, and mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCold TDP43 WT

   Plasmid

   #27451

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   Factor Xa, GST, and His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. PH-PLCD1(R40L)-GFP

   Plasmid

   #51408

   Purpose

   Non-PI(4,5)P2 binding control for PH-PLCD1-GFP

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

   Insert

   PH-PLCD1 (aa 1–170) (PLCD1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Arginine 40 mutated to leucine

   Promoter

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

8. ArcLight-Q239

   Plasmid

   #36856

   Depositor

   Vincent Pieribone

   Article

   Jin et al Neuron. 2012 Sep 6;75(5):779-85.

   Insert

   fluorescent protein voltage sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Ci-VSP contains R217Q mutation; super ecliptic pH…

   Promoter

   simian CMV

   Available since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX-evoCas9

   Plasmid

   #107550

   Purpose

   Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

   Depositor

   Anna Cereseto

   Article

   Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

   Insert

   Humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   M495V/Y515N/K526E/R661Q

   Promoter

   CBh

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. deltaNp63alpha-FLAG

    Plasmid

    #26979

    Depositor

    David Sidransky

    Article

    Chatterjee et al Cell Cycle. 2008 May 1. 7(9):1231-7.

    Insert

    ΔNP63alpha (TP63 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    dominant negative isoform of p63; aa 1–587

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFR-mApple

    Plasmid

    #203773

    Purpose

    Number and brightness analysis of EGFR oligomerization

    Depositor

    Thorsten Wohland

    Article

    Sankaran et al Nat Commun. 2021 Mar 19;12(1):1748. doi:

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCold-TF-hPLEKHM1

    Plasmid

    #64146

    Purpose

    For protein expression in E.coli

    Depositor

    Tamotsu Yoshimori

    Article

    Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

    Insert

    PLEKHM1 (PLEKHM1 Human)
    

    Use

    Tags

    6xHis-tag and Trigger Factor (TF)

    Expression

    Bacterial

    Mutation

    Promoter

    cspA

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pEGFP Rootletin (Nigg pFL2(CW499))

    Plasmid

    #41166

    Depositor

    Erich Nigg

    Article

    Bahe et al J Cell Biol. 2005 Oct 10;171(1):27-33. E

    Insert

    Rootletin (CROCC Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. GPI-RpHLuorin2

    Plasmid

    #171721

    Purpose

    GPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to GPI anchor
    

    Use

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. GA-NES

    Plasmid

    #61018

    Purpose

    ddGFP A-NES

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A
    

    Use

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. 014LP-pAAV-ANF-657- Cre-pA

    Plasmid

    #120279

    Purpose

    AAV vector for Atrial specific Cre recombinase expression

    Depositor

    Xander Wehrens

    Article

    Ni et al Circ Res. 2019 Jan 18;124(2):256-262. do

    Insert

    Cre
    

    Use

    AAV

    Tags

    CRE

    Expression

    Mutation

    Promoter

    ANF promoter

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid

    #201917

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and Human U6

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody

    Plasmid

    #138133

    Purpose

    Expresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    EGFP-anti-rabbit-IgG(Fc)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLJM1 Raptor GFP

    Plasmid

    #112744

    Purpose

    express Raptor-GFP

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Raptor-GFP (RPTOR Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21-pelB-LaTdT-39

    Plasmid

    #228980

    Purpose

    For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaTdT-39
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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