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pSB235

Plasmid

#164130

Purpose

PhlF-RFP expression plasmid

Depositor

Christopher Voigt

Publication

Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

Insert

PhlF-RFP

Expression

Bacterial

Promoter

Ptet

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBT13-splitD

Plasmid

#122597

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Publication

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pdCas9-J23109

Plasmid

#113151

Purpose

Plasmid constitutively expressing dCas9 protein

Depositor

Chong Zhang

Publication

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Insert

dCas9

Expression

Bacterial

Mutation

D10A & H840A

Available since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCD227

Plasmid

#113318

Purpose

AraC-pBAD-dCas9, MCP-SoxS_R93A

Depositor

Jesse Zalatan

Publication

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9 and MCP-SoxS_R93A

Use

CRISPR

Expression

Bacterial

Mutation

R93A

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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mCerulean-Lifeact-7

Plasmid

#54713

Purpose

Localization: Actin, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Insert

Lifeact-mCerulean

Expression

Mammalian

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Sp_PE

Plasmid

#212038

Purpose

Bacterial expression of the PE2 proteins for genome editing in E. coli

Depositor

Quanjiang Ji

Publication

Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

Insert

PE2

Use

CRISPR

Promoter

L-arabinose-inducible promoter PBAD

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGL003

Plasmid

#215335

Purpose

amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank promoter)

Depositor

Gene-Wei Li

Type

Empty backbone

Expression

Bacterial

Promoter

pSpank

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCsA

Plasmid

#136067

Purpose

Acceptor plasmid for even level position 1, lacZ blue-white screening

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pCAH01Sp::Cas9

Plasmid

#128160

Purpose

Ptet-controlled expression of S. pyogenes Cas9

Depositor

Calvin Henard

Publication

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Insert

Sp Cas9

Expression

Bacterial

Promoter

Ptet

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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2XDR4

Plasmid

#32615

Depositor

Connie Cepko

Publication

Billings et al PLoS One. 2010 . 5(10):e13739.

Insert

2XDR4

Expression

Mammalian

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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Cas12j-8-puro-crRNA

Plasmid

#194965

Purpose

The plasmid expresses human codon-optimized Cas12j-8, crRNA expression elements and puromycin resistence gene.

Depositor

Yongming Wang

Publication

Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAC-U63-QtgRNA2.1-BR

Plasmid

#170513

Purpose

gRNA cloning vector containing a BFP Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues.

Depositor

Chun Han

Publication

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

U6:3

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCDNA3.0_mitoNanobody

Plasmid

#136622

Purpose

Plasmid encoding outer mitochondrial localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion

Depositor

Kai Johnsson

Publication

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-EnhancerNb

Expression

Mammalian

Promoter

CMV

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMTB

Plasmid

#112225

Purpose

Expression of membrane-tagged super folder GFP from zebrafish beta-actin promoter.

Depositor

Sean Megason

Publication

Wagner et al Science. 2018 Apr 26. pii: science.aar43

Insert

Super-folder GFP

Use

Zebrafish expression

Promoter

beta actin 2 (zebrafish)

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAJM.714

Plasmid

#108515

Purpose

PVanCC-YFP Reporter. Van sensor for use in Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PVanCC-YFP

Expression

Bacterial

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD_A3001

Plasmid

#91042

Purpose

Module A, Promoter: 35S, Gene: GFP, Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFP

Use

Unspecified

Promoter

35S

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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UAS-myr::4xSNAPf

Plasmid

#87637

Purpose

For creating drosophila transgenics expressing UAS-myr4x::SNAPf

Depositor

Gregory Jefferis

Publication

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::4xSNAPf

Expression

Insect

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJZC583

Plasmid

#62314

Purpose

sgRNA with 2x MS2 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 2x MS2 binding module

Expression

Yeast

Promoter

SNR52

Available since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAcCas12nHS_RV6_empty

Plasmid

#203811

Purpose

Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

Depositor

Quanjiang Ji

Publication

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12nHS_RV6

Use

CRISPR

Promoter

CMV

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-CD4DARPin

Plasmid

#198294

Purpose

Produces PVCs retargeted with anti-CD4 DARPin D27.2

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-CD4DARPin

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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