239,468 results

1. pMA3790

   Plasmid

   #70110

   Purpose

   mtDNA elimination/production of rho-0 cells

   Depositor

   Mikhail Alexeyev

   Article

   Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

   Inserts

   EGFP
   hUNG1 Y147A
   Neo
   

   Tags

   None, human OTC mitochondrial targeting sequence,…

   Expression

   Mammalian

   Mutation

   Y147A, aa's 1-77 removed

   Promoter

   CMV, EF1a, and SV40 and native

   Available Since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-UbC-Tet1v4-dCas9

   Plasmid

   #232180

   Purpose

   Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

   Insert

   Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 S. pyogenes, Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   hUbC

   Available Since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-IGF1R-V5/HIS

   Plasmid

   #201979

   Purpose

   expression of human IGF1R receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   IGF1R receptor tyrosine kinase (IGF1R Human)
   

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPAGFP-N1

   Plasmid

   #11909

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

   Has Service

   Cloning Grade DNA

   Insert

   pPAGFP-N1
   

   Expression

   Mammalian

   Mutation

   These plasmids are essentially the same as the Cl…

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPAGFP-N1

   Plasmid

   #11909

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

   Has Service

   Cloning Grade DNA

   Insert

   pPAGFP-N1
   

   Expression

   Mammalian

   Mutation

   These plasmids are essentially the same as the Cl…

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCX137

   Plasmid

   #229212

   Purpose

   CRISPR array plasmid for NOTCH2 (Spacers 1-24)

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   CRISPR array plasmid for NOTCH2 (Spacers 1-24)
   

   Expression

   Mammalian

   Available Since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5-HA-Ubiquitin-K48R

   Plasmid

   #17604

   Purpose

   Mammalian expression of HA tagged ubiquitin K48R

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K48R (expected to disrupt G76-K48 ubiquitin chain…

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-HA-Ubiquitin-K48R

   Plasmid

   #17604

   Purpose

   Mammalian expression of HA tagged ubiquitin K48R

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K48R (expected to disrupt G76-K48 ubiquitin chain…

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-mCh-Cry2WT

   Plasmid

   #101221

   Purpose

   Blue-light dependent optogenetic protein Cry2

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

   Insert

   Cry2PHR
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-mCh-Cry2WT

    Plasmid

    #101221

    Purpose

    Blue-light dependent optogenetic protein Cry2

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    Cry2PHR
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJRH-1362 scFv entry plasmid

    Plasmid

    #201912

    Purpose

    Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Lyso-TORCAR

    Plasmid

    #64929

    Purpose

    Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    LAMP1-TORCAR (LAMP1 Synthetic, Human)
    

    Tags

    Cerulean, Full-length LAMP1, and YPet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Lyso-TORCAR

    Plasmid

    #64929

    Purpose

    Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    LAMP1-TORCAR (LAMP1 Human, Synthetic)
    

    Tags

    Cerulean, Full-length LAMP1, and YPet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid

    #139998

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti CMV rtTA3 Blast (w756-1)

    Plasmid

    #26429

    Purpose

    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Blast

    Depositor

    Eric Campeau

    Insert

    Tetracycline repressor A3 mutant
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti CMV rtTA3 Blast (w756-1)

    Plasmid

    #26429

    Purpose

    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Blast

    Depositor

    Eric Campeau

    Insert

    Tetracycline repressor A3 mutant
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFR WT

    Plasmid

    #11011

    Purpose

    Retroviral construct for expressing human EGFR in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFR WT

    Plasmid

    #11011

    Purpose

    Retroviral construct for expressing human EGFR in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-C3-hYAP1

    Plasmid

    #17843

    Depositor

    Marius Sudol

    Article

    Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

    Insert

    YAP (YAP1 Human)
    

    Tags

    EGFP

    Expression

    Bacterial and Mammalian

    Available Since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-C3-hYAP1

    Plasmid

    #17843

    Depositor

    Marius Sudol

    Article

    Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

    Insert

    YAP (YAP1 Human)
    

    Tags

    EGFP

    Expression

    Bacterial and Mammalian

    Available Since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge