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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,346 results for: multi

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Showing: 4521 - 4540 of 18346 results

  3. pCT

    Plasmid
    #60620
    Purpose
    Plasmid encoding iCas9 and tracrRNA on pRS415 backbone
    Depositor
    Huimin Zhao
    Publication
    Bao et al ACS Synth Biol. 2014 Sep 19.
    Inserts
    iCas9
    tracrRNA
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Yeast
    Mutation
    changed Aspartate 147 to Tyrosine, Proline 411 to…
    Promoter
    RPR1p and TEF1p
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    60620-plasmid-map-sequence-id-99574

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  5. pMax_mOrange_G299C

    Plasmid
    #177829
    Purpose
    Site-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).
    Depositor
    Zachary Nagel, Leona Samson
    Publication
    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mOrange_G299C
    Expression
    Mammalian
    Mutation
    G299C
    Promoter
    CMV
    Available since
    Nov. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    177829-plasmid-map-sequence-id-348954

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  6. pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (AAV1)

    Viral prep
    #198511-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (#198511). In addition to the viral particles, you will also receive purified pAAV_hSyn-DIO-PdCO-mScarlet-WPRE plasmid DNA. Syn-driven Cre-dependent expression of optimized PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Publication
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Promoter
    Syn
    Tags
    mScarlet (Cre-dependent)
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only

  7. pAAV-flex-taCasp3-TEVp (AAV1)

    Viral prep
    #45580-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-flex-taCasp3-TEVp (#45580). In addition to the viral particles, you will also receive purified pAAV-flex-taCasp3-TEVp plasmid DNA. EF1a-driven, Cre-dependent, bicicstronic expression of designer pro-taCasp3 and TEVp. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Nirao Shah, Jim Wells
    Publication
    Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10
    Promoter
    EF1a
    Available since
    Dec. 3, 2020
    Availability
    Academic Institutions and Nonprofits only

  8. pAAV-Ef1a-fDIO EYFP (AAV2)

    Viral prep
    #55641-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-Ef1a-fDIO EYFP (#55641). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO EYFP plasmid DNA. EF1a-driven, Flp-dependent expression of EYFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Publication
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    EF1a
    Tags
    EYFP (Flp-dependent)
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only

  10. pBUN501

    Plasmid
    #50582
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    zCas9D10A
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Mutation
    Cas9D10A nickase, was derived from the zCas9 and …
    Promoter
    AtU6-26p and Ubi1p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    50582-plasmid-map-sequence-id-75642

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  11. pRec

    Plasmid
    #104572
    Purpose
    Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI constitutively.
    Depositor
    Harris Wang
    Publication
    Sheth et al Science. 2017 Dec 15;358(6369):1457-1461
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PTet-cas12, tetR, lacI
    Use
    Synthetic Biology
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104572-plasmid-map-sequence-id-201411

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  16. pGLTR-X-PURO

    Plasmid
    #58246
    Purpose
    GATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and Puromycin selection
    Depositor
    Stephan Geley
    Publication
    Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:
    Insert
    Puro(R)
    Use
    Lentiviral and RNAi; Gateway destionation vector
    Tags
    TetR-NLS-T2A
    Expression
    Mammalian
    Promoter
    SFFV
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    58246-plasmid-map-sequence-id-89671

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  20. CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector

    Plasmid
    #159281
    Purpose
    A multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)
    Depositor
    Rudolf Jaenisch
    Publication
    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AsCpf1-HA-2A-GFP
    Use
    Multicistronic vector with both caggs promoter-dr…
    Tags
    3X HA
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    159281-plasmid-map-sequence-id-311914

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Showing: 4521 - 4540 of 18346 results