We narrowed to 13,280 results for: cDNA

1. Yellow Cameleon-Nano50/pcDNA3

   Plasmid

   #51964

   Purpose

   mammalian expression of Yellow Cameleon-Nano50, an ultrasensitive Ca2+ indicator with Kd = 50 nM

   Depositor

   Takeharu Nagai

   Article

   Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

   Insert

   Yellow Cameleon-Nano50
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Lyn-RAB-EKARev

   Plasmid

   #118477

   Purpose

   ddRFP-based single-color ERK biosensor targeted to the plasma membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   Lyn-RAB-EKARev
   

   Use

   Tags

   N-terminal targeting sequence from Lyn-kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1/TO-myc-BirA*-NES

   Plasmid

   #112714

   Purpose

   For tetracycline-inducible expression of myc-BirA*-NES in mammalian cells. For BioID studies.

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

   Insert

   HIV Rev nuclear export signal (NES)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. integrin beta6 pcDNA1 neo

   Plasmid

   #13580

   Depositor

   Dean Sheppard

   Article

   Weinacker et al J Biol Chem. 1994 Mar 4. 269(9):6940-8.

   Insert

   integrin beta 6 (ITGB6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA5/FRT-ChemoD-NAD

   Plasmid

   #193826

   Purpose

   CMV overexpression of ChemoD-NAD in mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoD-NAD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   ShadowG: T225R, HT7: L271E

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-MYO10-HMM-Nanotrap

   Plasmid

   #87255

   Purpose

   Expression plasmid to perform NanoSPD 2.0 assays in mammalian cells.

   Depositor

   Thomas Friedman

   Article

   Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

   Insert

   MYO10 (Myo10 Mouse)
   

   Use

   Tags

   GFP-nanotrap

   Expression

   Mammalian

   Mutation

   Myo10 is truncated to contain aa.1-941

   Promoter

   CMV

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.3 ss-FLAG-AP-hNDP (aa 25-133)

   Plasmid

   #115789

   Purpose

   Expresses and secretes human Norrin with N-terminal flag and alkaline phosphatase tags, contains artificial signal sequence upstream of tags.

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   NDP (NDP Human)
   

   Use

   Tags

   alkaline phosphatase and flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-FRT/TO-FLAG-SHLD3

   Plasmid

   #114123

   Purpose

   Expresses N-terminally tagged FLAG-SHLD3 in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   SHLD3 (SHLD3 Human)
   

   Use

   Genomic integration, tetracycline inducible

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available since

   Aug. 16, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. Human NCKX4-AS-long (pcDNA3.1+)

   Plasmid

   #75211

   Purpose

   Mammalian expression of SLC24A4

   Depositor

   Jonathan Lytton

   Article

   Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

   Insert

   SLC24A4 (SLC24A4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   alternatively spliced exon present

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-STIM1-Venus-173-C

    Plasmid

    #87619

    Purpose

    Full-length STIM1 fused to C-terminal Venus (YFP) fragment; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    STIM1-Venus173-C (STIM1 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMJA219= pcDNA3.1V5-completeTRABV-CMVp

    Plasmid

    #116878

    Purpose

    mammalian expression vector containing TCR sequences

    Depositor

    Marcos Alcocer

    Article

    Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

    Insert

    complete TRAV10-TRBV25-CMVp rabbitpolyA (TRAV10, TRBV25-1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-2xFLAG-2xSTREP-CCND1

    Plasmid

    #172648

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-AU1-mTOR-L1460P

    Plasmid

    #19995

    Depositor

    Fuyuhiko Tamanoi

    Article

    Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    mammalian target of rapamycin-L1460P (Mtor Rat)
    

    Use

    Tags

    AU1

    Expression

    Mammalian

    Mutation

    Changed Leucine 1460 to Proline

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pCDNA3-HA-Akt1-aa120-433

    Plasmid

    #73411

    Purpose

    Expresses HA tagged aa120-433 AKT1

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Expresses aa 120-433 of AKT1

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1(+)/UUG-nLuc-3XFLAG

    Plasmid

    #127302

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from UUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-FLAG-MTOR-T1977R

    Plasmid

    #69011

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-T1977R (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Threonine 1977 to Arginine

    Promoter

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA5/FRT/TO HIS HSPH2

    Plasmid

    #19535

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 4 (HSPA4 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    mutations A381T, T732A, and an insertion of "…

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3 Flag MKK4(ala)

    Plasmid

    #15314

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.

    Insert

    MKK4(ala) (MAP2K4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser-220Ala Thr-224Ala

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pCDNA3-deltaBamHI-human MIZ1

    Plasmid

    #74167

    Purpose

    Mammalian expression of human MIZ1

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    MIZ1 (ZBTB17 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1-Y1214F-VEGFR2-mCherry

    Plasmid

    #139609

    Purpose

    Expresses a mutant (Y1214F) VEGFR2-mCherry fusion protein in mammalian cells

    Depositor

    Michael Welsh

    Article

    Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

    Insert

    KDR (KDR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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