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Plasmid#166856PurposeHuman expression vector encoding full length SARS CoV-2 spike protein, containing biotinylation site and 8His-tag. Modification of Addgene plasmid #154754.DepositorInsertSARS CoV-2 HexaPro full length spike protein (S Severe acute respiratory syndrome-related coronavirus)
UseTags8xHis and Biotinylation siteExpressionMammalianMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus2-Y705F/S727A-STAT3
Plasmid#123177PurposeExpresses Y705F/S727A STAT3 double mutant fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
UseTagsVenus2 (aa 158-238) for bimolecular fluorescence …ExpressionMammalianMutationDouble Y705F and S727A substitutionsPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-2xFLAG-2xSTREP-CCND3-IRES-mCherry
Plasmid#172620PurposeLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D3 and mCherry in mammalian cellsDepositorInsertCCND3 (CCND3 Human)
UseLentiviralTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTT3-ecdICAM1-ST3-His
Plasmid#210666PurposeExpression of the extracellular domain of human ICAM1 fused to Spytag003 + Histag in HEK293 (or similar)DepositorInsertExtracellular domain of human ICAM-1 fused to Spytag003 and Histag (ICAM1 Synthetic, Human)
UseTagsHistag and Spytag003ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-Dnmt3a3L-sc27
Plasmid#71827PurposeFor bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.DepositorInsertDnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
UseTags6xHis tagExpressionBacterialMutationPromoterAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-BlastR [M1G]
Plasmid#171805PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and blasticidin resistance cassette [p.M1G]DepositorInsertmNeonGreen-T2A-BlastR
UseGene taggingTagsExpressionMutationBlastR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST EGFP-flag-CAD
Plasmid#188118PurposeExpresses N-terminal EGFP-tagged/C-terminal flag-tagged human CAD in mammalian cellsDepositorInsertCAD (CAD Human)
UseRetroviralTagsEGFP tag and Flag tagExpressionMammalianMutationTCC -> AGT silent mutations at nt439-441 elimi…PromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABE-Tau-cYFP
Plasmid#92205PurposeRetroviral overexpression vector for α-Syn/Tau bimolecular fluorescence complementation (BiFC)DepositorInsertMAPT (MAPT Human)
UseRetroviralTagsYFP(156-239)ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Venus1-Y705F/S727A-STAT3
Plasmid#123176PurposeExpresses Y705F/S727A STAT3 double mutant fused to the 1-157 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
UseTagsVenus1 (aa 1-158) for bimolecular fluorescence co…ExpressionMammalianMutationDouble Y705F and S727A substitutionsPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-2xFLAG-2xSTREP-CCND2-IRES-mCherry
Plasmid#172627PurposeLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D2 and mCherry in mammalian cellsDepositorInsertCCND2 (CCND2 Human)
UseLentiviralTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CDK6
Plasmid#172614PurposeExpresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cellsDepositorInsertCDK6 (CDK6 Human)
UseTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus1-S727A-STAT3
Plasmid#123174PurposeExpresses S727A STAT3 fused to the 1-157 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
UseTagsVenus1 (aa 1-158) for bimolecular fluorescence co…ExpressionMammalianMutationS727A substitutionPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1012 - pAAV EF1a DIO Nuc-eYFP
Plasmid#75082PurposeAn AAV packaging vector that expresses Cre-dependent nuclear-localized eYFP under control of the EF1a promoter.DepositorInsertNuc-eYFP
UseAAV and Cre/LoxTagsNLSExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-2xSTREP-CCND3(T283A)-Puro
Plasmid#172618PurposeRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(T283A) and a puromycin resistance marker in mammalian cellsDepositorInsertCCND3 (CCND3 Human)
UseRetroviralTagsFLAG-2xSTREPExpressionMammalianMutationThr283AlaPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-GLI1-P2A-mCherry
Plasmid#203858PurposeTet-inducible lentiviral plasmid expressing GLI1-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertGLI1 (GLI1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-2xSTREP-CCND3-Puro
Plasmid#172619PurposeRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cellsDepositorInsertCCND3 (CCND3 Human)
UseRetroviralTagsFLAG-2xSTREPExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABE-Tau-nYFP
Plasmid#92204PurposeRetroviral overexpression vector for Tau bimolecular fluorescence complementation (BiFC)DepositorInsertMAPT (MAPT Human)
UseRetroviralTagsYFP(1-155)ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-pGK-nAChRb2(E61C)-IRES-GFP
Plasmid#164777Purposecysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligandDepositorInsertnAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)
UseLentiviralTagsExpressionMammalianMutationE61CPromoterpGKAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
px458-CCND1
Plasmid#172630PurposeExpresses a gRNA against CCND1 and Cas9 from S. pyogenes with 2A-EGFPDepositorInsertgRNA against CCND1 (CCND1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irTNG4kwh
Plasmid#44722DepositorInsertspCMV-D2i promoter
htetR::NLS::EGFP
UseSynthetic Biology; Expression regulator/reporter;…TagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only