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pMXS-IRES-Blast POLE1 N-ISCmut

Plasmid

#160803

Purpose

Express N-terminal ISC mutant POLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLE1 N-ISCmut (POLE Human)

Use

Retroviral

Tags

3xFLAG

Mutation

shPOLE_1 and shPOLE2 resistant, C651S, C654S, C66…

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-MET

Plasmid

#116762

Purpose

Lentiviral expression of MET

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MET (MET Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-IGF1R

Plasmid

#116752

Purpose

Lentiviral expression of IGF1R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

IGF1R (IGF1R Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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PB-CAG-mRuby3-Gal8-P2A-Zeo

Plasmid

#150815

Purpose

Mammalian expression of mRuby3-Galectin8 (Gal8) fusion protein

Depositor

Jordan Green

Article

Rui et al Sci Adv. 2022 Jan 7;8(1):eabk2855. doi:

Insert

mRuby3-Gal8-P2A-Zeo (LGALS8 Synthetic, Human)

Tags

Gal8 is fused to the c-terminus of mRuby3

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-ESR1-Y537S

Plasmid

#116374

Purpose

Lentiviral expression of ESR1 Y537S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ESR1 (ESR1 Human)

Use

Lentiviral

Mutation

Y537S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-GATA3

Plasmid

#116747

Purpose

Lentiviral expression of GATA3

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

GATA3 (GATA3 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-H1047R

Plasmid

#116500

Purpose

Lentiviral expression of PIK3CA H1047R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

H1047R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pEJS1099: Dual-sgRNA.Design 4

Plasmid

#159537

Purpose

Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

Depositor

Erik Sontheimer

Article

Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

Insert

Nme2Cas9 with two guide RNA cassettes

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS

Expression

Mammalian

Promoter

U1a

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-CDK4

Plasmid

#116724

Purpose

Lentiviral expression of CDK4

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

CDK4 (CDK4 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Neuroligin-4* [N98/47R]

Plasmid

#188168

Purpose

Mammalian Expression Plasmid of anti-Neuroligin-4* (Mouse). Derived from hybridoma N98/47

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Neuroligin-4* (Mus musculus) recombinant mouse monoclonal antibody (Nlgn4l Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-DAPK1

Plasmid

#116727

Purpose

Lentiviral expression of DAPK1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

DAPK1 (DAPK1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-CTCF

Plasmid

#116728

Purpose

Lentiviral expression of CTCF

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

CTCF (CTCF Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-EGFP-PARP1

Plasmid

#176146

Purpose

EGFP fused to the N-terminus of PARP1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

Poly(ADP-Ribose) Polymerase 1 (PARP1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-V600E

Plasmid

#116204

Purpose

Lentiviral expression of BRAF V600E

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

V600E

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-en31FnCas9

Plasmid

#201949

Purpose

Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-en31FnCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

G1243T, E1369R, E1603H on FnCas9

Promoter

Cbh

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS1089: mini-AAV.sgRNA.Nme2Cas9

Plasmid

#159536

Purpose

Delivery of Nme2Cas9 and its sgRNA in a single AAV vector with overall packaging size of 4.4 Kb

Depositor

Erik Sontheimer

Article

Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

Insert

Nme2Cas9 with single guide RNA cassette

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS

Expression

Mammalian

Promoter

U1a

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FRT-Rev-TdTomato

Plasmid

#191203

Purpose

FlpO dependent TdTomato expression

Depositor

Janelia Viral Tools

Article

Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

Insert

TdTomato

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-AKT3

Plasmid

#116711

Purpose

Lentiviral expression of AKT3

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AKT3 (AKT3 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q42

Plasmid

#111746

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q42 (polyQ repeat)

Promoter

CMV and P10

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-KNL1-rvsf_aaaa-dCas9

Plasmid

#248567

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

KNL1-rvsf_aaaa-dCas9

Use

CRISPR

Tags

HA, 2xNLS

Expression

Mammalian

Mutation

KNL1 RVSF58-61AAAA; Cas9 D10A, H840A

Promoter

CMV, TetO2

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pHAGE-3xmScarlet-dCas9

Plasmid

#248561

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

dCas9

Use

CRISPR

Tags

3xmScarlet and HA, 2xNLS

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CMV, TetO2

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-7xTRE-3xeGFP

Plasmid

#191206

Purpose

GFP expression under the control of TRE promotor

Depositor

Janelia Viral Tools

Article

Glaser et al Nat Methods. 2022 May;19(5):613-619. doi

Insert

3X GFP

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-E545K

Plasmid

#116485

Purpose

Lentiviral expression of PIK3CA E545K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

E545K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB3

Plasmid

#116735

Purpose

Lentiviral expression of ERBB3

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB3 (ERBB3 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE

Plasmid

#125713

Purpose

Cre-dependent vector expressing an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

Depositor

Ofer Yizhar

Article

Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

Has Service

AAV1 and AAV5

Insert

eOPN3

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

deleted amino acids 331-429

Promoter

hSyn1

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-en1FnCas9

Plasmid

#201947

Purpose

Mammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-en1FnCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

E1369R on FnCas9

Promoter

Cbh

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS1096 Dual-sgRNA.Design 1

Plasmid

#159538

Purpose

Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

Depositor

Erik Sontheimer

Article

Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

Insert

Nme2Cas9 nuclease with two guide RNA cassettes with promoters

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS

Expression

Mammalian

Promoter

U1a

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-EZH2

Plasmid

#116738

Purpose

Lentiviral expression of EZH2

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EZH2 (EZH2 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSpp1-is2

Plasmid

#58248

Purpose

Expression vector producing isoform 2 derived mouse osteopontin (EGFP tagged)

Depositor

Georgios Stathopoulos

Article

Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

Insert

Spp1-is2 (Spp1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-EPHA2

Plasmid

#116732

Purpose

Lentiviral expression of EPHA2

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EPHA2 (EPHA2 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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