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We narrowed to 12,282 results for: LEA

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Showing: 4561 - 4580 of 12282 results

  1. 2xMARS-nGFP

    Plasmid
    #205235
    Purpose
    Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells
    Depositor
    Jeremy Baskin
    Publication
    Cao et al Nat Cell Biol. 2024 Oct;26(10):1804-1816
    Insert
    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    Tags
    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205235-plasmid-map-sequence-id-404746

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  3. pAAV2-CAG-3xNLS-AausFP1

    Plasmid
    #191096
    Purpose
    To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Publication
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-AausFP1
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    Optimized to Human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    191096-plasmid-map-sequence-id-380123

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  5. 6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)

    Plasmid
    #169168
    Purpose
    Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.
    Depositor
    Sascha Martens
    Publication
    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017
    Insert
    MAP1LC3B (MAP1LC3B Human)
    Tags
    6X Histidine Tag, TEV cleavage site, mCherry
    Expression
    Bacterial
    Promoter
    T7 lac promoter
    Available since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    169168-plasmid-map-sequence-id-332048

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  8. pGBW-m4046879

    Plasmid
    #149238
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 nsp2
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149238-plasmid-map-sequence-id-357573

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  9. pGBW-m4046809

    Plasmid
    #145685
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145685-plasmid-map-sequence-id-297845

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  10. pGBW-m4046933

    Plasmid
    #145592
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp7
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145592-plasmid-map-sequence-id-292573

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  11. pelB-MBP-P66-ss

    Plasmid
    #137045
    Purpose
    E. coli expression clone (T7lac promoter) for mature P66 (aa 22-618) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66949.1 (p66 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature P66: lacks the P66 signal peptide
    Promoter
    T7lac
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137045-plasmid-map-sequence-id-291951

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  12. pLL3.7-EF-EYFP-YAP1_WW-PolyA

    Plasmid
    #112290
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) WW mutant. WQDP (199-202) in the first WW domain was changed to AQDA, and the WLDP (258-261) of the second WW domain was changed to ALDA
    Depositor
    Erik Sahai
    Publication
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Mutation
    Human YAP1-2gamma with mutations in the WW domain…
    Promoter
    EF-1 alpha
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    112290-plasmid-map-sequence-id-213664

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  13. pCEP4-DU422gp140-TM

    Plasmid
    #123251
    Purpose
    Mammalian expression plasmid for soluble gp140 from the DU422 HIV-1 isolate with a transmembrane tether
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (DU422) Env
    Tags
    CD5 leader peptide and Linker (with 6his tag) and…
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    123251-plasmid-map-sequence-id-235207

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  14. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231G

    Plasmid
    #113953
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Publication
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, I132S, S212N, and …
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113953-plasmid-map-sequence-id-215167

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  15. pCEP4-gp120(BaL)-CD4

    Plasmid
    #98957
    Purpose
    Mammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolate
    Depositor
    Erik Procko
    Publication
    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (BaL) gp120
    Tags
    CD4 (domains D1-D2), CD5 leader sequence, FLAG ep…
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    98957-plasmid-map-sequence-id-193406

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  17. pSc1

    Plasmid
    #80436
    Purpose
    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell
    Depositor
    Ervin Welker
    Publication
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    sp Cas9 gRNA
    eGFP
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available since
    Aug. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    80436-plasmid-map-sequence-id-151445

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  18. GFP-Drosha1-850

    Plasmid
    #62522
    Purpose
    C-terminal aa851-1374 of Drosha is deleted
    Depositor
    Bharat Ramratnam
    Publication
    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human Drosha with aa 851-1374 deleted (DROSHA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    deleted amino acids 851-1374
    Available since
    March 20, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. GRLF1

    Plasmid
    #36195
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GRLF1 (Codon Devices Synthesized) (ARHGAP35 Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    Contains the Ras homolog domain only (amino acid …
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 4561 - 4580 of 12282 results