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pJL1-CyPet

Plasmid

#102636

Purpose

In vitro expression of CyPet from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

CyPet

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mM-Asn209Asp_M18

Plasmid

#91781

Purpose

Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Asp (EU numbering).

Depositor

Joanna Bereta

Article

Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

Insert

immunoglobulin heavy constant mu (Ighm Mouse)

Expression

Mammalian

Mutation

Asn209 changed to Asp (EU numbering)

Promoter

hEF1-HTLV prom

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mM-Asn209Ala_M18

Plasmid

#91733

Purpose

Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ala (EU numbering).

Depositor

Joanna Bereta

Article

Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

Insert

immunoglobulin heavy constant mu (Ighm Mouse)

Expression

Mammalian

Mutation

Asn209 changed to Ala (EU numbering)

Promoter

hEF1-HTLV prom

Available Since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Flag-PolB(K244A/T304I)-Puro

Plasmid

#177144

Purpose

Lentiviral vector expressing Flag-PolB(K244A/T304I) and a puromycin resistance cassette

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pChuk

Plasmid

#87033

Purpose

express IKKalpha in mammalian cells

Depositor

Georgios Stathopoulos

Article

Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

Insert

IKKalpha (Chuk Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) FITM2

Plasmid

#107504

Purpose

Expresses FITM2, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

FITM2 (codon optimized)

Use

Lentiviral

Expression

Mammalian

Mutation

Codon optimized sequence

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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3294_pETcon-SARS2-RBD_Omicron-BA1

Plasmid

#184408

Purpose

yeast surface display of the SARS-CoV-2 Omicron BA.1 variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Omicron BA.1 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

G339D+S371L+S373P+S375F+K417N+N440K+G446S+S477N+T…

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_hE2F7mg_115mut

Plasmid

#73077

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette) with inserted SNORD115 binding site

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns, m…

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCW-MRTFA-HA

Plasmid

#247436

Purpose

siRNA resistant HA-tagged mouse MRTF-A cloned into the pCW backbone (Addgene #41393, with puromycin resistance) together with an IRES GFP to indicate doxycycline-inducible expression by fluorescence.

Depositor

Richard Treisman

Article

Nielsen et al J Cell Sci. 2025 Nov 7:jcs.264444. doi:

Inserts

MRTF-A (Mrtfa Mouse)
IRES2:EGFP

Use

Lentiviral; Dox-inducible

Tags

HA

Expression

Mammalian

Mutation

siRNA resistant - no amino acid mutations

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLex307-UCOE-SFFV-puroR-MCP-HSF1-VIRF2

Plasmid

#218557

Purpose

Encodes SFFV promoter-driven MCP-HSF1-VIRF2 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

Depositor

Alejandro Chavez

Article

Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi

Insert

MCP-HSF1-VIRF2

Use

Lentiviral

Tags

SV40NLS

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA893 - pBA904 Puro-T2A-BFP KLF5 g1 CRISPRa guide guide (pRCA594 backbone) 666

Plasmid

#238186

Purpose

Lentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

KLF5 sgRNA CRISPRa (KLF5 Synthetic, Human)

Use

CRISPR and Lentiviral

Tags

mTagBFP2

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs_ZCRB1 (pAVA3300)

Plasmid

#239325

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNA stargeting ZCRB1

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting ZCRB1 (ZCRB1 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Nav1.6(K1425TAG)-HA

Plasmid

#239763

Purpose

Expresses mouse Nav1.6 with a TAG codon at position 1425 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Scn8a (Scn8a Mouse)

Tags

HA-tag

Expression

Mammalian

Mutation

K1425TAG

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Nav1.6(K1546TAG)-HA

Plasmid

#239764

Purpose

Expresses mouse Nav1.6 with a TAG codon at position 1546 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Scn8a (Scn8a Mouse)

Tags

HA-tag

Expression

Mammalian

Mutation

K1546TAG

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA860 - pBA904 Puro-T2A-GFP EGR3 g1 CRISPRa guide guide (pRCA360 backbone)

Plasmid

#238188

Purpose

Lentiviral CRISPR guide vector expressing a EGR3 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

EGR3 sgRNA CRISPRa (EGR3 Synthetic, Human)

Use

CRISPR and Lentiviral

Tags

GFP

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA805 - pBA904 Puro-T2A-GFP KLF4 g1 CRISPRa guide (pRCA360 backbone) 668

Plasmid

#238174

Purpose

Lentiviral CRISPR guide vector expressing a KLF4 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

KLF4 sgRNA CRISPRa (KLF4 Synthetic, Human)

Use

CRISPR and Lentiviral

Tags

GFP

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA803 - pBA904 Puro-T2A-GFP KLF5 g1 CRISPRa guide (pRCA360 backbone) 666

Plasmid

#238172

Purpose

Lentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

KLF5 sgRNA CRISPRa (KLF5 Synthetic, Human)

Use

CRISPR and Lentiviral

Tags

GFP

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA804 - pBA904 Puro-T2A-GFP KLF5 g2 CRISPRa guide (pRCA360 backbone) 665

Plasmid

#238173

Purpose

Lentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

KLF5 sgRNA CRISPRa (KLF5 Synthetic, Human)

Use

CRISPR and Lentiviral

Tags

GFP

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRCA895 - pBA904 Puro-T2A-BFP KLF4 g1 CRISPRa guide guide (pRCA594 backbone) 668

Plasmid

#238187

Purpose

Lentiviral CRISPR guide vector expressing a KLF4 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

KLF4 sgRNA CRISPRa (KLF4 Synthetic, Human)

Use

CRISPR and Lentiviral

Tags

mTagBFP2

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-SAVED-CHAT-cbm

Plasmid

#214032

Purpose

Bacterial expression plasmid for strep-tagged SAVED-CHAT cA3-binding pocket mutant from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT

Tags

Strep-tag II

Expression

Bacterial

Mutation

K121E, Q122A, N274A, R276E, Y285A, F286A

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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