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Showing: 1101 - 1150 of 1449 results

  12. S+Q111R+N122D

    Plasmid
    #167175
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R and N122D mutations in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-S+Q111R+N122D
    ATP1B1
    Expression
    Bacterial and Insect
    Mutation
    Changed Q at 111 to R and changed N at 122 to D
    Promoter
    P10 and PH
    Available Since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167175-plasmid-map-sequence-id-334477

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  13. S+10subs

    Plasmid
    #167176
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 10 R mutations in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-S+10R
    ATP1B1
    Expression
    Bacterial and Insect
    Mutation
    Changed A at 112 to T; E at 116 to D; I at 135 to…
    Promoter
    P10 and PH
    Available Since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167176-plasmid-map-sequence-id-345188

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  14. S+12subs

    Plasmid
    #167177
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 12 R mutations in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-S+12R
    ATP1B1
    Expression
    Bacterial and Insect
    Mutation
    Changed Q at 111 to R; A at 112 to T; E at 116to …
    Promoter
    P10 and PH
    Available Since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167177-plasmid-map-sequence-id-334485

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  17. pLentiCMV_HyPer7RgDAAO

    Plasmid
    #217653
    Purpose
    Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane
    Depositor
    Seth Parker
    Article
    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi
    Insert
    HyPer7 D amino acid oxidase
    Use
    Lentiviral
    Tags
    Nuclear export signal
    Expression
    Mammalian
    Mutation
    Fused DAAO to the C-terminus of HyPer7 using a Gl…
    Promoter
    CMV
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217653-plasmid-map-sequence-id-441459

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  23. pMSCVpuro-Mito-Pericam

    Plasmid
    #87381
    Purpose
    Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.
    Depositor
    Björn Stork
    Article
    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do
    Insert
    Mito-Pericam [ratiometric]
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    Retroviral 5' LTR promoter
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    87381-plasmid-map-sequence-id-212242

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  27. pSN840

    Plasmid
    #184832
    Purpose
    Expresses human KIF5A(∆exon27) and human KLC1 in insect cells
    Depositor
    Kyoko Chiba
    Article
    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g
    Inserts
    KIF5A (∆exon27) (KIF5A Human)
    KLC1 (KLC1 Human)
    Use
    Cre/Lox
    Tags
    His-FLAG and mScarlet-2xStrepII
    Expression
    Insect
    Mutation
    ∆exon27 form of human KIF5A
    Promoter
    p10 and polyhedrin
    Available Since
    July 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    184832-plasmid-map-sequence-id-367218

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  33. hSyn-HA-PGRN-LAMP1TM-IRES-GFP

    Plasmid
    #234877
    Purpose
    Lentiviral plasmid expressing HA-tagged human progranulin that is fused to the transmembrane domain and cytosolic tail of LAMP-1. Enables lysosomal delivery of progranulin without secretion.
    Depositor
    Andrew Arrant
    Article
    Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi
    Insert
    Progranulin (GRN Human)
    Use
    Lentiviral
    Tags
    HA tag and LAMP-1 transmembrane domain and cytoso…
    Expression
    Mammalian
    Promoter
    hSYn
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234877-plasmid-map-sequence-id-468254

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  35. pAAV hSyn mCh-IRES-SspB(milli)-BoNT/B(147-441, Y365A)

    Plasmid
    #122985
    Purpose
    AAV plasmid with human synapsin promoter driving mCh and SspB(milli)-BoNT/B amino acids 147-441 separated with an IRES element. Co-express with BoNT/B(1-146)-iLID construct for PA-BoNT
    Depositor
    Matthew Kennedy
    Article
    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)
    Insert
    mCh-IRES-SspB(milli)-BoNT/B(147-441)
    Use
    AAV
    Tags
    mCh
    Expression
    Mammalian
    Mutation
    SspB contains A58V,R73Q "milli" mutatio…
    Promoter
    human synapsin
    Available Since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    122985-plasmid-map-sequence-id-238429

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  37. pAAV-hSyn-DIO {hCAR}off-{DTR-GFP}on-W3SL

    Plasmid
    #111396
    Purpose
    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation), and W3SL cassette (for maximize cloning capacity)
    Depositor
    Adam Kepecs
    Article
    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi
    Inserts
    hCAR (CXADR Human)
    DTR-EGFP
    Use
    AAV
    Tags
    EGFP and Myc
    Expression
    Mammalian
    Promoter
    hSynapsin
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    111396-plasmid-map-sequence-id-212262

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  38. pAAV hSyn mCh-IRES-SspB(micro)-BoNT/B(147-441, Y365A)

    Plasmid
    #122986
    Purpose
    AAV plasmid with human synapsin promoter driving mCh and SspB(micro)-BoNT/B amino acids 147-441 separated with an IRES element. Co-express with BoNT/B(1-146)-iLID construct for PA-BoNT
    Depositor
    Matthew Kennedy
    Article
    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)
    Insert
    mCh-IRES-SspB(micro)-BoNT/B(147-441)
    Use
    AAV
    Tags
    mCh
    Expression
    Mammalian
    Mutation
    SspB contains R73Q "micro" mutation, Bo…
    Promoter
    human synapsin
    Available Since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    122986-plasmid-map-sequence-id-238430

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  42. MB 103C ttrSR(m13)-Bxb1_P7-sfGFP_mCherry

    Plasmid
    #232475
    Purpose
    Optimized tetrathionate sensor with recombinase switch and fluorescent reporter (GFP), constitutive mCherry
    Depositor
    Mikhail Shapiro
    Article
    Buss et al Nat Commun. 2025 Aug 25;16(1):7931. doi:
    Inserts
    ttrS
    ttrR
    PttrB185-269
    sfGFP
    AxeTxe
    mCherry
    Bxb1 integrase
    Tags
    ssrA degradation tag
    Expression
    Bacterial
    Promoter
    Constitutive (TTGATAGCTAGCTCAGTCCTAGGTATTGTGCTAGC…
    Available Since
    Nov. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232475-plasmid-map-sequence-id-493218

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  43. MB 45t3 thsS(t3)R-sfGFP_mCherry

    Plasmid
    #232470
    Purpose
    Optimized thiosulfate sensor with fluorescent reporter (GFP), constitutive mCherry
    Depositor
    Mikhail Shapiro
    Article
    Buss et al Nat Commun. 2025 Aug 25;16(1):7931. doi:
    Inserts
    thsS(t3)
    thsR
    PphsA342
    sfGFP
    AxeTxe
    mCherry
    Expression
    Bacterial
    Mutation
    Contains the following mutations K286Q, Q350H, I…
    Promoter
    J23100 (TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGC), J23…
    Available Since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    232470-plasmid-map-sequence-id-467797

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  44. pLV-EF1A-WWE-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro

    Plasmid
    #187611
    Purpose
    Split luciferase construct with nLuc fused to C-terminus of WWE domain, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii
    Inserts
    WWE domain with C-terminal nLuc
    WWE domain with C-terminal cLuc
    Use
    Lentiviral
    Tags
    FLAG, HA, Split luciferase (cLuc), Split lucifera…
    Expression
    Mammalian
    Promoter
    EF1A
    Available Since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    187611-plasmid-map-sequence-id-372189

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  45. pLV-EF1A-WWE(R163A)-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro

    Plasmid
    #187612
    Purpose
    Split luciferase construct with nLuc fused to C-terminus of WWE domain with Arg163Ala mutation, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii
    Inserts
    WWE domain (R163A) with C-terminal nLuc
    WWE domain with C-terminal cLuc
    Use
    Lentiviral
    Tags
    FLAG, HA, Split luciferase (cLuc), Split lucifera…
    Expression
    Mammalian
    Mutation
    Arg163Ala
    Promoter
    EF1A
    Available Since
    Sept. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187612-plasmid-map-sequence-id-372035

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Showing: 1101 - 1150 of 1449 results