We narrowed to 1,821 results for: pCAG

1. pCAG-OSF-PP-squirrel-monkey-CHMP3

   Plasmid

   #154182

   Purpose

   expresses squirrel monkey CHMP3 in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

   Insert

   CHMP3
   

   Use

   Tags

   FLAG, Strep-Tag II, and preScission site

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-OSF-PP-squirrel-monkey-retroCHMP3

   Plasmid

   #154184

   Purpose

   expresses squirrel monkey retroCHMP3 in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

   Insert

   retroCHMP3
   

   Use

   Tags

   FLAG, Strep-Tag II, and preScission site

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCaggs-NLS-PAmCherry1-GSS-EGFP

   Plasmid

   #158000

   Purpose

   Expression of nucleus localized PAmCherry1 and EGFP fusion protein

   Depositor

   Francois St-Pierre

   Article

   Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

   Insert

   NLS-PAmCherry1-GSS-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Actin promoter with CMV enhancer

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAAA)

   Plasmid

   #137860

   Purpose

   Monomeric TagBFP2 and iCre-ERT2 recombinase (PAAA N-terminus)

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAAAA)

   Plasmid

   #137861

   Purpose

   Monomeric TagBFP2 and iCre-ERT2 recombinase (PAAAA N-terminus)

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSA)

   Plasmid

   #137862

   Purpose

   Monomeric TagBFP2 and iCre-ERT2 recombinase (PAGSA N-terminus)

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAAGSA)

   Plasmid

   #137863

   Purpose

   Monomeric TagBFP2 and iCre-ERT2 recombinase (PAAGSA N-terminus)

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAGGS-mTagBFP2-T2A-iCreERT2 (PVV)

   Plasmid

   #128172

   Purpose

   Monomeric TagBFP2 and iCre-ERT2 recombinase (PVV N-terminus)

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAGGS-mTagBFP2-T2A-iCreERT2 (PVGSA)

   Plasmid

   #128173

   Purpose

   Monomeric TagBFP2 and iCre-ERT2 recombinase (PVGSA N-terminus)

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBW759_pCAG-PV2-lox2272-GFP-BGHpA

    Plasmid

    #87575

    Purpose

    PV2 for 2-input, 4-output decoder circuit and 3-input, 2-output Full Adder

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    EGFP
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBW807_pCAG-PV4-loxP-mCherry-BGHpA

    Plasmid

    #87583

    Purpose

    PV4 for 3-input, 2-output Full Adder

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mCherry
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBW770_pCAG-PV4-loxP-mRuby2-BGHpA

    Plasmid

    #87577

    Purpose

    PV4 for 2-input, 4-output decoder circuit

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mRuby2
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBW707_pCAG-PV3-FRT-loxP-mCherry

    Plasmid

    #87571

    Purpose

    PV3 for 2-input, 2-output circuit library

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mCherry
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBW705_pCAG-PV1-FRT-lox2272-STOP

    Plasmid

    #87569

    Purpose

    PV1 for 2-input, 2-output circuit library

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    Neomycin Resistance Gene
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-Archon1-KGC-EGFP-ER2-WPRE

    Plasmid

    #108423

    Purpose

    Archon1 fluorescent voltage reporter in mammalian expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-KGC-EGFP-ER2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEN396 - pCAGGS-Tir1-V5-2A-PuroR TIGRE donor

    Plasmid

    #92142

    Purpose

    Targeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection (2A-fusion). Auxin-inducible degron system.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    osTir1
    

    Use

    Mouse Targeting

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid

    #139999

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CAG

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-ERT2-PhoCl-Gal4VP16-PhoCl-ERT2

    Plasmid

    #87693

    Purpose

    Express ERT2-PhoCl-Gal4VP16-PhoCl-ERT2 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    ERT2-PhoCl-Gal4VP16-PhoCl-ERT2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid

    #139998

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

    Promoter

    CAG

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)

    Plasmid

    #223249

    Purpose

    Mammalian expression of the optimized R2Tg RNA donor

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg donor_opt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAGEN-WPRE-bGH-DEST (JDW 1309)

    Plasmid

    #229815

    Purpose

    A CAGGS driven, gateway compatible destination vector that contains a WPRE downstream of the destination cassette to stabilize transcripts

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

    Plasmid

    #140001

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9-NG with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

    Promoter

    CAG

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAGGS DRR mCherry Donor EF1a BFP

    Plasmid

    #98896

    Purpose

    Homologous recombination donor for pDRR. Expresses mCherry after repait by HR.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    Neomycin-T2A-mCherry-GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mCherry-pCAG-donor(R2Tg)-GFP(intron)

    Plasmid

    #223248

    Purpose

    Mammalian expression of the R2Tg RNA donor

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV pCAG-FLEX2-tTA2-WPRE-bGHpA

    Plasmid

    #65458

    Purpose

    Can be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    tTA2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-pCAG eGFP T2A SP HALO HAPLN1

    Plasmid

    #228200

    Purpose

    AAV vector expressing GFP and HaloTag-HAPLN1 under constitutive promoter

    Depositor

    Sungjin Park

    Article

    Sterin et al J Neurosci. 2024 Sep 9:e0666242024. doi:

    Insert

    HAPLN1 (Hapln1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    constitutive CAG promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAGGS-mEGFP-loxP-P2A-dTKneo-pA-loxP

    Plasmid

    #139484

    Purpose

    PCR template for mEGFP gene knock-in donor vector

    Depositor

    Kazuhiro Aoki

    Article

    Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

    Insert

    mEGFP-loxP-P2A-dTKneo-pA-loxP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAGGS puro-GFP-BamHI-IRES-mCherry

    Plasmid

    #232247

    Purpose

    AcGFP1-BamHI-IRES-mCherry empty backbone

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG-lox-roxSTOProx-lyn-mScarlet-pA-lox

    Plasmid

    #196889

    Purpose

    Dre-activated Cre-inactivated expression of lyn-mScarlet reporter.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    lyn-mScarlet
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKS070 - pCAGGS-3XFLAG-(human)CTCF-eGFP

    Plasmid

    #156448

    Purpose

    For transient expression of human CTCF tagged with eGFP

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    human CTCF (CTCF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

    Plasmid

    #220493

    Purpose

    To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.

    Depositor

    Sébastien Britton

    Article

    Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

    Insert

    sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pKS035 - pCAGGS-3XFLAG-Rad21-mKate2-bpA

    Plasmid

    #156445

    Purpose

    For transient expression of mouse RAD21 (SCC1) tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse RAD21 (Rad21 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)

    Plasmid

    #127392

    Purpose

    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D/T305A/T306A)

    Depositor

    Ryohei Yasuda

    Article

    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

    Insert

    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    changed Threonine 286 to Aspartic acid and Threon…

    Promoter

    pCAG

    Available since

    July 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCAG-Synaptophysin-TdTomato-IRES2-OHT-Cre

    Plasmid

    #134308

    Purpose

    Expresses Synaptophysin-TdTomato and also tamoxifen dependant Cre (OHT-Cre, aka Cre-ER)

    Depositor

    Elly Nedivi

    Article

    Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

    Inserts

    Synaptophysin (Syp Rat, Mouse)
    Cre-ER
    

    Use

    Tags

    TdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pKS037 - pCAGGS-3XFLAG-Smc3-mKate2-bpA

    Plasmid

    #156447

    Purpose

    For transient expression of mouse SMC3 tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse SMC3 (Smc3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination

    Plasmid

    #40132

    Purpose

    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).

    Depositor

    Pawel Pelczar

    Article

    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

    Type

    Empty backbone

    Use

    Tags

    FokI nuclease (ELD)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination

    Plasmid

    #40131

    Purpose

    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).

    Depositor

    Pawel Pelczar

    Article

    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

    Type

    Empty backbone

    Use

    Tags

    FokI nuclease (KKR)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA

    Plasmid

    #193968

    Purpose

    Encodes a wildtype human Pol? (eta) with P2A-tagBFP marker driven by CAG promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    hPol eta (POLH Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2

    Plasmid

    #87694

    Purpose

    Express ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    ERT2-PhoCl-Cre-PhoCl-ERT2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCAG-Halo-Tag-128-425-Akap9

    Plasmid

    #196873

    Purpose

    Expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to Halo-Tag. Used to displace endogenous Akap9 from Golgi

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Halo-Tag-Akap9 (128-425) (Akap9 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pKS021 - pCAGGS-3XFLAG-mKate2-SA1-bpA

    Plasmid

    #156440

    Purpose

    For transient expression of mouse SA1 (= STAG1 or SCC3A) tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse SA1 (Stag1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCAG-Archon2-KGC-GFP-ER2-WPRE

    Plasmid

    #108424

    Purpose

    Archon2 fluorescent voltage reporter in mammalian expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon2-KGC-EGFP-ER2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCAG-FLAG-puro-PTPIP51(1-150_175-470)

    Plasmid

    #170537

    Purpose

    Expresses PTPIP51 FFAT-like motif (151-175) deletion mutant (1-150_176-470) in human HeLa cells after transfection

    Depositor

    Byung Il Lee

    Article

    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

    Insert

    Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    deleted amino acids 151-175

    Promoter

    CAG promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

44. pKS031 - pCAGGS-3XFLAG-BORIS-eGFP-bpA

    Plasmid

    #156443

    Purpose

    For transient expression of mouse BORIS (CTCFL) tagged with eGFP

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse BORIS (Ctcfl Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCAGGS-FLAG-BMAL1-S513A/S515A/S516A

    Plasmid

    #186835

    Purpose

    Expresses FLAG-BMAL1-S513A/S515A/S516A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 513/515/516 to Alanine

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCAGGS-Halotag-loxP-P2A-dTKneo-pA-loxP

    Plasmid

    #139485

    Purpose

    PCR template for Halo-Tag gene knock-in donor vector

    Depositor

    Kazuhiro Aoki

    Article

    Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

    Insert

    HaloTag-loxP-P2A-dTKneo-pA-loxP
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCAGGS-mNG-eDHFR(69K6)-G-alpha-q(L254A)

    Plasmid

    #178851

    Purpose

    Mammalian expression of Gαq(L254A) fused to mNeonGreen-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    mNeonGreen-eDHFR(69K6)-Gαq(L254A) (GNAQ Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Gαq: C9S/C10S/Q209L/L254A

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCAG-hCjeCas9-NLS(SV40)-3xFLAG (KAC579)

    Plasmid

    #133789

    Purpose

    CAG promoter expression plasmid for human codon optimized CjeCas9 nuclease with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized CjeCas9
    

    Use

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CAG

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pKS016 - pCAGGS-3XFLAG-mKate2-Pds5a-bpA

    Plasmid

    #156439

    Purpose

    For transient expression of mouse PDS5A tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse PDS5A (Pds5a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pKS023 - pCAGGS-3XFLAG-mKate2-Pds5b-bpA

    Plasmid

    #156442

    Purpose

    For transient expression of mouse PDS5B tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse PDS5B (Pds5b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge