We narrowed to 45,419 results for: uros

1. pRP(Exp)-CMV> ORF_924bp (Azgp1):T2A:dTomato:IRES:Puro

   Plasmid

   #110830

   Purpose

   Expresses zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter where both sequences are separated by a T2A self-cleaving peptide sequence as a result, the dT is not fused with the ZAG protein.

   Depositor

   Ande Satyanarayana

   Article

   Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

   Insert

   AZGP1 (Azgp1 Mouse)
   

   Use

   Non-viral gene expression vector

   Tags

   T2A and dTomato

   Promoter

   CMV

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBabe puro-HA-PIK3CA(H1047R;F486S;del)

   Plasmid

   #33255

   Depositor

   Thomas Roberts

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   Hemagglutinin (HA)

   Expression

   Mammalian

   Mutation

   changed histidine 1047 to arginine and phenylalan…

   Promoter

   SV40

   Available Since

   Jan. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

   Plasmid

   #138525

   Purpose

   Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.

   Depositor

   Richard Jenner

   Article

   Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

   Insert

   Control non-PRC2 binding A-tract repeat RNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP

   Plasmid

   #75123

   Purpose

   Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4 with a C terminal 3x FLAG-tag

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   BRD9 (BRD9 Human)
   

   Use

   Retroviral

   Tags

   3x FLAG-tag

   Mutation

   bromodomain replaced with the first bromodomain o…

   Available Since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

   Plasmid

   #164800

   Purpose

   For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   ERH CRISPRi gRNA1
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha and U6

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

   Plasmid

   #164801

   Purpose

   For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   ERH CRISPRi gRNA2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha and U6

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZIP-hCMV-ZsGreen-P2A-Puro-NT4

   Plasmid

   #123328

   Purpose

   Lentiviral vector for expression of non-targeting control shRNA #4 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

   Insert

   non-targeting control shRNA embedded in miR30 backbone
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-Flag-Polß(D256A)-IRES-puro

   Plasmid

   #128677

   Purpose

   mammalian expression of Flag-Polß(D256A) protein with puromycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D256A

   Promoter

   CMV

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV-6NBC-GFP-PGK-Puro-Barcode1

   Plasmid

   #83678

   Purpose

   individual 6N barcode to make barcoded cell line variants

   Depositor

   Monte Winslow

   Article

   Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

   Insert

   Barcode1
   

   Use

   Retroviral

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-HIF2α P405A/P531A/N847Q-pBabe-Puro

    Plasmid

    #25948

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Available Since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti CMV Puro DEST (w118-1) VOPP1

    Plasmid

    #107507

    Purpose

    expressing VOPP1, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    VOPP1 (VOPP1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRetroX-Tight-puro-3xFLAG-RUNX3 R122C

    Plasmid

    #203439

    Purpose

    Mammalian expression of RUNX4

    Depositor

    Yoshiaki Ito

    Article

    Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

    Insert

    RUNX3 (RUNX3 Human)
    

    Tags

    3 x FLAG

    Expression

    Mammalian

    Mutation

    R122C

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe-TetCMV-puro-mVenus-PA-Rac1-T17N

    Plasmid

    #22018

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mVenus-PA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Rac1 starts at I4 and contains mutation T17N.

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pCDH-CB-FLPe-P2A-copGFP-T2A-Puro

    Plasmid

    #72258

    Purpose

    Express FLPe, copGFP and Puromycin resistance gene under CB promoter

    Depositor

    Kazuhiro Oka

    Insert

    FLPe-P2A-copGFp-T2A-Puro
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CB and chicken beta-actin + CMV enhancer

    Available Since

    Jan. 15, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLenti-C-Myc-DDK-IRES-Puro HNRNPR

    Plasmid

    #200730

    Purpose

    Lentiviral expression of Flag-Tagged human HNRNPR

    Depositor

    Claudio Alarcón

    Insert

    HNRNPR (HNRNPR Human)
    

    Use

    Lentiviral

    Tags

    Myc-DDK from backbone plasmid

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-Cb5-IRES-puromycin-pLVx-EF1a

    Plasmid

    #177434

    Purpose

    To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.

    Depositor

    David Andrews

    Insert

    Cb5 tail anchor (Cyb5r4 Rat)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-CAG-FLEX-NEUROD1-T2A-mCherry

    Plasmid

    #178584

    Purpose

    Cre-dependent expression of NEUROD1 and mCherry under the constitutive promoter

    Depositor

    Chun-Li Zhang

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

    Insert

    NEUROD1 (NEUROD1 Human)
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract

    Plasmid

    #138524

    Purpose

    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.

    Depositor

    Richard Jenner

    Article

    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

    Insert

    PRC2-binding G-tract repeat RNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV-EZH2_EZH1SET-PGK-Puro-IRES-GFP

    Plasmid

    #75126

    Purpose

    Retroviral expression plasmid encoding a human EZH2 SET-domain-swap mutant containing the EZH1 SET domain

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    KMT6 (EZH2 Human)
    

    Use

    Retroviral

    Tags

    None

    Mutation

    SET domain replaced with the SET domain of EZH1

    Available Since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

20. nonphosphorylatable Mis12-targeted Aurora B FRET sensor

    Plasmid

    #45229

    Depositor

    Michael Lampson

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    non phosphorylatable Mis12-targeted Aurora B FRET sensor
    

    Expression

    Mammalian

    Mutation

    T-A mutation in substrate sequence

    Available Since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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