We narrowed to 6,250 results for: nls

1. pCMV-mA3-BE3

   Plasmid

   #113419

   Purpose

   Expresses mA3-BE3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   mA3-BE3 (Apobec3 S. pyogenes and Bacteriophage PBS2, Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-hA3G-BE3

   Plasmid

   #113415

   Purpose

   Expresses hA3G-BE3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3G-BE3 (APOBEC3G S. pyogenes and Bacteriophage PBS2, Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNS20-SpCas9-SNAP

   Plasmid

   #113717

   Purpose

   Bacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9

   Depositor

   Gerald Schwank

   Article

   Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   HA-2xNLS-SNAP-NLS and His6-MBP-TEV

   Expression

   Bacterial

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJEP304-pAAV-EFS-dSaCas9-VP64-pA

   Plasmid

   #113679

   Purpose

   A EFS driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   de-catalyzed SaCas9
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   NLS and VP64

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pY31

   Plasmid

   #84746

   Purpose

   Expresses huLbCpf1-P2A-puro and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huLbCpf1
   puromycin resistance gene
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV N-SpyCas9-Intein, U6-gRNA scaffold (F+E)

   Plasmid

   #120293

   Purpose

   AAV Vector for expression of N-terminal SpyCas9 fragment with Intein and a U6-driven F+E gRNA scaffold

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   N-terminal fragment of SpyCas9
   

   Use

   AAV and CRISPR

   Tags

   SV40-NLS and split-intein

   Expression

   Mammalian

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAB(EXPR-PYL-HDAC3-NEO)

   Plasmid

   #114394

   Purpose

   For PYL-HDAC3 expression

   Depositor

   Vincent Dion

   Article

   Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

   Insert

   PYL-HDAC3 (HDAC3 Human)
   

   Tags

   3xFlag-NLS (internal)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLM-AMA18.0 dCas9-VPR

   Plasmid

   #138945

   Purpose

   Fungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta (VPR); ribozymes based sgRNA transcription unit; Terbinafine and Phleomycin selection markers

   Depositor

   Arnold Driessen

   Article

   Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10

   Insert

   p40S:sp_dCas9m4_2xNLS-VPR; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
   

   Use

   CRISPR and Synthetic Biology; Ama1 autonomously r…

   Tags

   NLS

   Promoter

   p40S (AN0465) Aspergillus nidulans

   Available Since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmiCas9-msa

   Plasmid

   #164833

   Purpose

   miCas9-msa fusion protein expressing plasmid

   Depositor

   Eugene Chen

   Article

   Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

   Insert

   miCas9-msa
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Mammalian

   Mutation

   contains mSA sequence range is 5106-5447 and Brex…

   Promoter

   CMV

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLL8 lenti-D10Acas9-3xflag-blast

    Plasmid

    #112134

    Purpose

    lenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE)

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    D10Acas9-3xflag-blast
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A mutant in Cas9

    Promoter

    EF1a

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pY095

    Plasmid

    #84744

    Purpose

    Expresses huLbCpf1-T2A-GFP and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huLbCpf1
    GFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-BE4GamRA-P2A-Puro

    Plasmid

    #112673

    Purpose

    Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE4GamRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    EF1s

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFPN1-N-gamma-tubulin (334-449)

    Plasmid

    #87859

    Purpose

    Fluorescent fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLS

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    gamma-tubulin 1 (TUBG1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    fragment from aa 334 to 449

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJW1836

    Plasmid

    #154337

    Purpose

    Promoter reporter

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    SV40 NLS::mScarlet_I::PEST (dpi)::-tbb-2 3'UTR
    

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505)

    Plasmid

    #139995

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    HF1=N497A/R661A/Q695A/Q926A

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-hA3A-BE3-D131Y

    Plasmid

    #113424

    Purpose

    Expresses hA3A-BE3-D131Y in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3A-BE3-D131Y (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_D131Y

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. XLone-BSD Cas13d P2A mCherry

    Plasmid

    #155183

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d and mCherry

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    CasRx
    

    Use

    Unspecified; Piggybac transposon

    Tags

    NLS and P2A mCherry

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNS38-SadCas9-SNAP

    Plasmid

    #113718

    Purpose

    Bacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA-2xNLS-SNAP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    D10A, N580A

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. A3G-BE5.14

    Plasmid

    #163638

    Purpose

    C to T DNA base editing

    Depositor

    Xue Gao

    Article

    Lee et al Sci Adv. 2020 Jul 15;6(29):eaba1773. doi

    Insert

    A3G-BE5.14
    

    Tags

    BPSV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-hA3H-BE3

    Plasmid

    #113416

    Purpose

    Expresses hA3H-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3H-BE3 (APOBEC3H S. pyogenes and Bacteriophage PBS2, Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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