We narrowed to 6,508 results for: nls
-
Plasmid#138160PurposeC-to-T base editorDepositorInsertrAPOBEC1(YE1)-nSpCas9 (CP1028) -UGI-UGI
TagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E; within Cas9 (CP1028…PromoterCMVAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(EF1a)
Plasmid#172326PurposeLV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an EF1a promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianPromoterEF1-alphaAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK/CMV)
Plasmid#172330PurposeLV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK/CMV promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianPromoterPGK/CMVAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
(564) pAAV Alb-AAT KRAB-SadCas9 U6-mPcsk9
Plasmid#163025PurposeExpression of CRISPRi with gRNA against mouse Pcsk9DepositorInsertMammalian codon-optimized SadCas9 (Pcsk9 S. aureus)
UseAAVTagsKRAB, SV40 pA, and myc NLSExpressionMammalianPromoterAlb-AATAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAT105
Plasmid#180510PurposePlasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacersDepositorInsertsCasX sgRNAv2
DpbCasX with R3 loop
UseCRISPRTags2x FLAG and SV40 NLSExpressionMammalianPromoterCAG and U6Available SinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MP-84-spCas9-U6-gRNA
Plasmid#206935PurposeExpression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.DepositorInsertspCas9
UseAAVTagsSV40 NLSExpressionMammalianPromoterMP-84Available SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS406-CMV-lacI-3FLAG
Plasmid#83410PurposeExpression of lacI in yeastDepositorInsertlacI repressor
Tags3xFLAG and NLSExpressionYeastPromoterCMVAvailable SinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGem-nHyPer:cAPX
Plasmid#84736PurposeSimultaneous expresion of stromal HyPer2 and cytosolic Ascorbate peroxidase (APX) in plant cellsDepositorInsertsTagsNLS-SV40ExpressionPlantPromoterpFMV/p35SAvailable SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spCas9-ABE-Umax-rest1
Plasmid#222144PurposeAdenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafishDepositorInsertModified TadA-nSpCas9
UseCRISPR; Zebrafish expressionTagsBipartite NLSPromoterT3Available SinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICH47742:FCP:Cas9YFP
Plasmid#85986PurposeGolden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoterDepositorInsertCas9
UseCRISPR; Golden gate assemblyTagsSV40 NLS, YFPPromoterFCPAvailable SinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ284 (Mb2Cas12a)
Plasmid#138116PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a without promoterDepositorInsertMb2Cas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE4-RrA3F F130L
Plasmid#138341PurposeMammalian expression plasmid for BE4-RrA3F F130LDepositorInsertBE4-RrA3F F130L
UseSynthetic BiologyTagsBP-NLSPromoterCMVAvailable SinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX394 Neisseria cinerea Cas9
Plasmid#68331PurposeNeisseria cinerea Cas9DepositorInsertNcCas9
UseCRISPRTagsHA and NLSExpressionMammalianPromoterCMVAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pY007 (pcDNA3.1-hPeCpf1)
Plasmid#69979PurposeExpresses humanized PeCpf1DepositorInserthPeCpf1
UseCRISPRTagsNLS & 3xHAExpressionMammalianPromoterCMVAvailable SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pY125 (pcDNA3.1-huBfCpf1)
Plasmid#92301PurposeExpression of humanized BfCpf1DepositorInserthuBfCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY115 (pcDNA3.1-huMlCpf1)
Plasmid#92275PurposeExpression of humanized MlCpf1DepositorInserthuMlCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEMS1505
Plasmid#29300PurposePleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).DepositorInsertPle34 (CLDN5 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsIntron-LacZ-NLSAvailable SinceNov. 4, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-BE4RA-P2A-Puro
Plasmid#112672PurposeLentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized)DepositorInsertBE4RA
UseLentiviralTagsFLAGMutationNLS sequence at the N-terminus and D10APromoterEF1sAvailable SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pY119 (pcDNA3.1-huLb5Cpf1)
Plasmid#92295PurposeExpression of humanized Lb5Cpf1DepositorInserthuLb5Cpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY123 (pcDNA3.1-huCMaCpf1)
Plasmid#92299PurposeExpression of humanized CMaCpf1DepositorInserthuCMaCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only