167,910 results

1. RAR-TRE-mStrawberry reporter

   Plasmid

   #158683

   Purpose

   This plasmid is a RAR pathway reporter. It has a Retinoic Acid Receptor Response Element promoter driving the expression of mStrawberry-pGK-BSD

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTcI-Wasabi

   Plasmid

   #86101

   Purpose

   mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TcI
   mWasabi
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A67T

   Promoter

   pR/pL and pR/pL, LacI

   Available Since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBbi_RP_MicroID2-HA

   Plasmid

   #188521

   Purpose

   Encodes the biotin proximity labeling enzyme MicroID2 for expression and stable integration into mammalian cells

   Depositor

   James Londino

   Article

   Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

   Insert

   MicroID2
   

   Use

   Transposon mediated gene integration

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF_PE2

   Plasmid

   #199267

   Purpose

   Mammalian expression of SpCas9 prime editor 2 under EF1α promoter

   Depositor

   Ting Zhou

   Article

   Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

   Insert

   PE2-P2A-mp53DD
   

   Expression

   Mammalian

   Mutation

   Δ40-903

   Available Since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV_CAG_FLEX_PKIαmut-P2A-mRuby3_WPRE

   Plasmid

   #196678

   Purpose

   AAV plasmid for mammalian Cre-dependent expression of PKIαmut-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIαmut bears the R20G/R21G mutation that renders it non-functional.

   Depositor

   Haining Zhong

   Article

   Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

   Insert

   PKIαmut-P2A-mRuby3
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-HA-USP5

   Plasmid

   #22590

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP5 (USP5 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT3-EF1a-c-Met

   Plasmid

   #31784

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   Met (MET Human)
   

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. HOP-flash

   Plasmid

   #83467

   Purpose

   TEAD-YAP/TAZ tx activity reporter. 8xwt TEAD binding sites with minimal promoter plus luciferase reporter gene

   Depositor

   Barry Gumbiner

   Article

   Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

   Insert

   Multimerized TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
   

   Use

   Luciferase

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. FUW-Sox10

   Plasmid

   #36978

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   Sox10 (SOX10 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti_PEmax-2A-MLH1dn-2A-BSD

    Plasmid

    #234072

    Purpose

    For lentiviral expression of PEmax with MLH1dn and blasticidin resistance

    Depositor

    Greg Findlay

    Article

    Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

    Insert

    PEmax-2A-MLH1dn
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    EF-1α core promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CaMKIIa-eArchT3.0-P2A-EGFP-WPRE-hGHpA

    Plasmid

    #51110

    Purpose

    Forebrain principal neuron expression of eArchT3,0 with physically uncoupled EGFP fluorophore

    Depositor

    Jonathan Ting

    Insert

    eArchT3.0
    

    Use

    AAV

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Promoter

    CaMKIIa

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMRXIP-GFP-PI4K2A

    Plasmid

    #221760

    Purpose

    Stable expression of GFP-PI4K2A in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    PI4K2A (PI4K2A Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGP-AAV-syn-jGCaMP8m-WPRE

    Plasmid

    #162375

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-FLEX-tdTomato (AAV8)

    Viral Prep

    #28306-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Promoter

    CAG

    Tags

    tdTomato (Cre-dependent)

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

15. pT2/HB

    Plasmid

    #26557

    Depositor

    Perry Hackett

    Insert

    pT2/HB
    

    Expression

    Bacterial

    Available Since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-PEmax

    Plasmid

    #187893

    Purpose

    All-in-one prime editor piggyBac transposon with PEmax

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    BPSV40 NLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-CAG-hChR2-H134R-tdTomato

    Plasmid

    #28017

    Purpose

    AAV-mediated expression of hChR2(H134R) for neuronal excitation.

    Depositor

    Karel Svoboda

    Article

    Mao et al Neuron. 2011 Oct 6;72(1):111-23.

    Has Service

    AAV Retrograde

    Insert

    humanized ChR2-H134R-tdTomato
    

    Use

    AAV; Adeno associate viral

    Tags

    tdTomato

    Mutation

    H134R

    Promoter

    CAG

    Available Since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTwist GFP-Linker-VDAC1

    Plasmid

    #211735

    Purpose

    Expresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the N-terminus of VDAC1 with a flexible linker.

    Depositor

    Roland Malli

    Article

    Pilic et al bioRxiv 2023.09.08.556841

    Insert

    Voltage-dependent anion channel 1 (VDAC1 )
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-mPIAS1

    Plasmid

    #15206

    Depositor

    Ke Shuai

    Article

    Liu et al Proc Natl Acad Sci U S A. 1998 Sep 1. 95

    Insert

    PIAS1 (Pias1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. AICSDP-15:ACTB-mEGFP

    Plasmid

    #87425

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    ACTB Homology Arms with mEGFP-linker (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    March 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. peYFP-C1-mTOR

    Plasmid

    #73384

    Purpose

    Expresses YFP tagged mTOR

    Depositor

    Jie Chen, Taekjip Ha

    Article

    Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

    Insert

    mTOR (MTOR Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pP2-P1(S')

    Plasmid

    #196334

    Purpose

    Plasmid expressing chimeric P2-P1(S') tail fiber

    Depositor

    Baojun Wang

    Article

    Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

    Insert

    chimeric tail fiber P2-P1(S') and chaperone protein U'
    

    Expression

    Bacterial

    Promoter

    P2 late promoter

    Available Since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCVD442

    Plasmid

    #11074

    Depositor

    Michael Donnenberg, James Kaper

    Article

    Donnenberg et al Infect Immun. 1991 Dec . 59(12):4310-7.

    Insert

    sacB (sacB Bacillus subtilis)
    

    Expression

    Bacterial

    Available Since

    Jan. 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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24. M6PR-bio-His

    Plasmid

    #51924

    Purpose

    Expresses full-length Cation-dependent mannose-6-phosphate receptor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    M6PR (M6PR Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. LZ10 PBREBAC-H2BHalo

    Plasmid

    #91564

    Purpose

    piggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.

    Depositor

    James Zhe Liu

    Article

    Li et al Elife. 2016 Aug 3;5. pii: e17056. doi: 1

    Insert

    histone H2B-HaloTag
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAAV-ihSyn1-tTA

    Plasmid

    #99120

    Purpose

    An AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTA

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Has Service

    AAV1

    Insert

    tTA
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    ihSyn1

    Available Since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. PiggyBac-FLAG-hTET2-IRES-Blast

    Plasmid

    #239819

    Purpose

    PiggyBac vector to express full length human TET2 with 2X N-terminal FLAG tags

    Depositor

    Kasper Rasmussen

    Article

    Knatko et al Blood Adv. 2025 Jun 24:bloodadvances.202

    Insert

    FLAG-tagged human TET2
    

    Tags

    2x FLAG tag on TET2

    Expression

    Mammalian

    Available Since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCDNA5/FRT/TO_H2B_HaloTag7

    Plasmid

    #169329

    Purpose

    Expression of H2B-HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag7 (H2BC21 Human)
    

    Tags

    HaloTag7

    Expression

    Mammalian

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pRL623

    Plasmid

    #58494

    Purpose

    helper plasmid for bacterial conjugal DNA transfer

    Depositor

    Peter Wolk

    Article

    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

    Insert

    encodes methylases AvaiM, Eco47iiM, and Ecot22iM that target sites subject to restriction in Anabaena sp. strain PCC 7120
    

    Available Since

    Oct. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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30. pDEST17-Cdc34a

    Plasmid

    #18674

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Cdc34A (CDC34 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only