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164,728 results

Showing: 6961 - 6990 of 164728 results

  2. pAAV-hsyn-NE2h

    Plasmid
    #208687
    Purpose
    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h in neurons
    Depositor
    Yulong Li
    Has Service
    AAV5
    Insert
    GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    208687-plasmid-map-sequence-id-412174

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  3. pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher

    Plasmid
    #117656
    Purpose
    Bacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.
    Depositor
    Bo Huang
    Article
    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.
    Inserts
    sfCherry2(1-10)
    sfCherry2(11)-SpyCatcher002
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    117656-plasmid-map-sequence-id-226764

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  6. pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64

    Plasmid
    #203350
    Purpose
    Template for T7 in vitro transcription of NanoLuc mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail
    Depositor
    Molly Stevens, Christopher Grigsby
    Insert
    Nano Luciferase
    Use
    Template for t7-mediated in vitro transcription o…
    Tags
    Flanked by 5' and 3' UTRs from Xenopus …
    Expression
    Mutation
    Promoter
    T7
    Available since
    June 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    203350-plasmid-map-sequence-id-399148

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  7. pVITRO1-dV-IgE/λ

    Plasmid
    #61879
    Purpose
    Human immunoglobulin epsilon antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    Human immunoglobulin Epsilon constant region
    Human immunoglobulin lambda constant region
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deleted Variable region
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61879-plasmid-map-sequence-id-333601

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  9. pAAV_hSyn-PdCO-EGFP-WPRE (AAV1)

    Viral Prep
    #198513-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV_hSyn-PdCO-EGFP-WPRE (#198513). In addition to the viral particles, you will also receive purified pAAV_hSyn-PdCO-EGFP-WPRE plasmid DNA. Synapsin-driven expression of the optimized opsin PdCO in frame with EGFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Promoter
    Syn
    Tags
    EGFP
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only

  10. Lyso-B

    Plasmid
    #209873
    Purpose
    Dimerization dependent fluorescent protein B anchored to cytosolic face of the lysosomal membrane with human LAMP1
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP B
    Use
    Tags
    LAMP1
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209873-plasmid-map-sequence-id-415450

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  11. mEmerald-Annexin-12

    Plasmid
    #53991
    Purpose
    Localization: Plasma Membrane, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Annexin (ANXA4 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. pLV-dCas9-p300-P2A-PuroR

    Plasmid
    #83889
    Purpose
    Lentiviral Sp dCas9-p300-P2A-PuroR
    Depositor
    Charles Gersbach
    Article
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    pac from Streptomyces alboniger
    Use
    CRISPR and Lentiviral
    Tags
    Flag and nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    83889-plasmid-map-sequence-id-297764

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  19. pHS2056

    Plasmid
    #239837
    Purpose
    OMEGAOff + omegaRNA scaffold AAV expression
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    DNMT3A-DNMT3L-NovaIscB-KRAB::NovaIscB omegaRNA scaffold
    Use
    AAV
    Tags
    HA and SV40 NLS
    Expression
    Mutation
    Insertion of REC domain from NbaCas9-1 + E137K/E4…
    Promoter
    EFS; human U6
    Available since
    July 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    239837-plasmid-map-sequence-id-479103

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  20. CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

    Plasmid
    #225756
    Purpose
    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection
    Depositor
    Paul Blainey
    Article
    Walton et al bioRxiv 2024.03.17.585235
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    225756-plasmid-map-sequence-id-446064

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  21. pCFD5_w

    Plasmid
    #112645
    Purpose
    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.
    Depositor
    Michael Boutros
    Insert
    U6:3-tRNA-sgRNA expression cassette
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112645-plasmid-map-sequence-id-217990

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  22. pENN.AAV.CB7.CI.eGFP.WPRE.rBG

    Plasmid
    #105542
    Purpose
    AAV expression of EGFP from CB7 promoter
    Depositor
    James M. Wilson
    Has Service
    AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CB7
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105542-plasmid-map-sequence-id-201063

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  24. MAPK1

    Plasmid
    #73248
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAPK1 (MAPK1 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73248-plasmid-map-sequence-id-137205

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  26. pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA

    Plasmid
    #105648
    Purpose
    Expression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeks
    Depositor
    Sandra Rieger
    Insert
    Tol2-Isl2b:GFP:polyA (isl2b Zebrafish)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    105648-plasmid-map-sequence-id-202869

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Showing: 6961 - 6990 of 164728 results