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Addgene

We narrowed to 7,266 results for: 11

Showing: 4641 - 4660 of 7266 results
  1. ARB366

    Plasmid
    #124049
    Purpose
    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector
    Depositor
    Insert
    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::KRAB-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. ARB367

    Plasmid
    #124050
    Purpose
    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector
    Depositor
    Insert
    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pMCh-623

    Plasmid
    #92266
    Purpose
    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 6W6A with six mutated W-motifs in its N-terminal domain in D. melanogaster cells
    Insert
    dGW182 6W6A (gw Fly)
    Use
    Tags
    NHA
    Expression
    Insect
    Mutation
    The following point mutations were introduced W23…
    Promoter
    Ac5
    Available since
    Dec. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
  4. STARR-seq luciferase validation vector_ORI_GTSE1

    Plasmid
    #99303
    Purpose
    Luciferase validation vector with GTSE1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Insert
    enhancer; chr22: 46718429 -46719913
    Use
    Luciferase; Starr-seq luciferase validation vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
  5. STARR-seq luciferase validation vector_mP_GTSE1

    Plasmid
    #99304
    Purpose
    Luciferase validation vector with GTSE1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Insert
    enhancer; chr22: 46718429 -46719913
    Use
    Luciferase; Starr-seq luciferase validation vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. STARR-seq luciferase validation vector_SCP1_GTSE1

    Plasmid
    #99305
    Purpose
    Luciferase validation vector with GTSE1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Insert
    enhancer; chr22: 46718429 -46719913
    Use
    Luciferase; Starr-seq luciferase validation vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
  7. pMCh-660

    Plasmid
    #92259
    Purpose
    Plasmid for expression of NHA-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cells
    Insert
    TNRC6C CED (TNRC6C Human)
    Use
    Tags
    NHA
    Expression
    Mammalian
    Mutation
    C-terminal effector domain (CED): aa 1369-1690 wi…
    Promoter
    CMV
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. pMCh-665

    Plasmid
    #92254
    Purpose
    Plasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cells
    Insert
    TNRC6C CED (TNRC6C Human)
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    C-terminal effector domain (CED): aa 1369-1690 wi…
    Promoter
    EF1A
    Available since
    Oct. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 4641 - 4660 of 7266 results