We narrowed to 7,648 results for: Lif;

1. pSCL.102

   Plasmid

   #184987

   Purpose

   Express -Eco1 RT-P2A-Cas9

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1RT-P2A-Cas9
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLS.544

   Plasmid

   #184961

   Purpose

   Test effect of a1/a2 length on editing murF using retron recombineering

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 22
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   murF donor G1359A, a1/a2 length extended to 22 bp

   Promoter

   T7/lac

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSCL.94

   Plasmid

   #184985

   Purpose

   Express -Eco1 RT/Cas9 fusion, using linker2

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1RT-fusion_linker2-Cas9
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSCL.006

   Plasmid

   #184970

   Purpose

   Express -Eco1 RT in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Integrating inducible cassette: Eco1 RT
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSCL.005

   Plasmid

   #184971

   Purpose

   Express Cas9 in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Integrating inducible cassette: Cas9
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   Promoter

   Gal1-10

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSLS.543

   Plasmid

   #184960

   Purpose

   Test effect of a1/a2 length on editing murF using retron recombineering

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   murF donor G1359A

   Promoter

   T7/lac

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSCL.71

   Plasmid

   #184983

   Purpose

   Express -Eco1 RT/Cas9 fusion, using linker1

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1RT-fusion_linker1-Cas9
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSLS.545

   Plasmid

   #184962

   Purpose

   Test effect of a1/a2 length on editing priB using retron recombineering

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   priB donor G1069A

   Promoter

   T7/lac

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. KBB106

   Plasmid

   #185065

   Purpose

   Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion including C-terminal half of Nup1 C-terminal FG domain

   Depositor

   Ken Belanger

   Article

   Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

   Insert

   NUP1
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Nup1 C-terminal region, truncated so C-terminal h…

   Promoter

   Available since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMC-9-35S-11

    Plasmid

    #176881

    Purpose

    MoClo Level 0 plasmid, contains 35S

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    35S terminator
    

    Use

    Tags

    Expression

    Plant

    Mutation

    WT

    Promoter

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLNHA-C1-HsNot8_J

    Plasmid

    #146699

    Purpose

    Mammalian Expression of HsNot8

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

    Insert

    HsNot8 (CNOT8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX458_sgRNA_Ago1_5

    Plasmid

    #172470

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1

    Depositor

    Constance Ciaudo

    Article

    Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

    Insert

    sgAgo1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX458_sgRNA_Ago1_6

    Plasmid

    #172471

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1.

    Depositor

    Constance Ciaudo

    Article

    Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

    Insert

    sgAgo1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS2+ gcsf

    Plasmid

    #132947

    Purpose

    Expresses zebrafish granulocyte colony stimulating factor (csf3a)

    Depositor

    Jason Berman

    Article

    Rajan et al Leukemia. 2021 Sep 30. pii: 10.1038/s413

    Insert

    gcsf (csf3a Zebrafish)
    

    Use

    Mrna synthesis

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. C453-E18: attB4-polyhedrinp>-attB5

    Plasmid

    #162977

    Purpose

    Gateway attB4/attB5 entry clone for baculovirus expression using the AcMNPV polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pZS160

    Plasmid

    #134238

    Purpose

    Lentiviral plasmid expressing sgRNA against HJURP

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    Human HJURP sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKM328

    Plasmid

    #134243

    Purpose

    Mammalian expression construct expressing sgRNA against PLK1

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    gRNA targeting PLK1 3'
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMET17p-CCG 316

    Plasmid

    #131166

    Purpose

    For ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

    Depositor

    Nils Johnsson

    Article

    Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

    Type

    Empty backbone

    Use

    Tags

    CCG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWPI-TFDP2

    Plasmid

    #114300

    Purpose

    Constitutive expression of the TFDP2 protein

    Depositor

    Patrick Salmon

    Article

    Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

    Insert

    TFDP2 (TFDP2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV(shRNA)-Puro-6 CCEPR Vb2

    Plasmid

    #108278

    Purpose

    shRNA for CCEPR lncRNA

    Depositor

    Karl Munger

    Article

    Sharma et al Virology. 2018 Feb 7;518:8-13. doi: 10.1

    Insert

    CCEPR (CCEPR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge