-
Plasmid#162564PurposeMeasuring cPLA2 activation in live cells after heat shock.DepositorUseGateway donor vectorTagsmK2ExpressionMutationPromotercmlc2Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only
-
pAAV-Syn-FLEX-rc [ChromeT-GFP]
Plasmid#153539PurposeAAV-mediated expression of ChromeT-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.DepositorInsertChromeT-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterSynAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Oct4deltaPE-53BP1mCherry
Plasmid#141122PurposeConstruct used to generate a transgenic mouse strain with expression of 53BP1-mCherry in embryonic germ cellsDepositorInsertFragment of human 53BP1 (TP53BP1 Human)
UseMouse TargetingTagsmCherryExpressionMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AvicFP3
Plasmid#129506PurposeExpresses AvicFP3 constitutively in E. coli (most strains)DepositorInsertAvicFP3
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-RNF68 [dRING]
Plasmid#126549PurposeExpresses FLAG-HA-tagged RNF68 [dRING mutant] in mammalian cellsDepositorInsertRNF68 (PCGF1 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationRING domain deletion mutant [dRING mutation is a …PromoterLTRAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-RNF68 [Y252D]
Plasmid#126550PurposeExpresses FLAG-HA-tagged RNF68 [Y252D] mutant in mammalian cellsDepositorInsertRNF68 (PCGF1 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationY252D point mutantPromoterLTRAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK_P-GAP-LM_lacO-cP-AOX1-sAR
Plasmid#126745Purposeyeast plasmid overexpressing sLovA,CPR and LacI-Mit1ADDepositorInsertslovA,cpr
UseTags6xHisExpressionYeastMutationPromoterGAPAvailable sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK_P-ICL1-LM_lacO-cP-AOX1-sAR
Plasmid#126744Purposeyeast plasmid overexpressing sLovA,CPR and LacI-Mit1ADDepositorInsertslovA,cpr
UseTags6xHisExpressionYeastMutationPromoterICL1Available sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT3038
Plasmid#122545PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a lysosomal related organelle localized gene (glo-1) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_glo-1::CemOrange2 (glo-1 Synthetic, Nematode)
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT3043
Plasmid#122546PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian LC3 (sqst-1) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemOrange2::lgg-1 (lgg-1 Synthetic, Nematode)
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 RR837-8GG
Plasmid#113958Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
UseTagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationresidues 22-839, R837G and R838G substitutionPromotercmvAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
FN(9-10, RGE)-I27-CC
Plasmid#85396PurposeMTFM mechanosensor for integrin. Fibronectin domains 9&10 with RGE mutation fused to titan I27 domain with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labelingDepositorInsertFibronectin (FN) domains 9&10 containing RGE mutation fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
UseTags6x HisExpressionBacterialMutationGRGDS motif mutated to GRGESPromoterT7Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
GAL-p300-1901-2414
Plasmid#89103Purposemammalian expression of human p300 amino acids 1901-2414 with N-terminal GAL4 DNA binding domainDepositorInsertp300 (EP300 Human)
UseTagsGAL4 DBDExpressionMammalianMutationaa 1901-2414PromoterAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160E,NoCys)
Plasmid#66875PurposeExpresses a cysteine-free version of pET30Xa-SBL1(160E) (4Cys to Ser) with N-term HistagDepositorInsertsoybean lipoxygenase-1 (S160E) with all Cys to Ser mutations
UseTagsHistagExpressionBacterialMutationS160E; changed 4 (all) cysteines to serinePromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONL-C1_fibrillarin
Plasmid#65710PurposeExpresses ONL-fibrillarin in mammalian cellsDepositorInsertfibrillarin (FBL Human)
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYNL-C1_fibrillarin
Plasmid#65708PurposeExpresses YNL-fibrillarin in mammalian cellsDepositorInsertfibrillarin (FBL Human)
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-FYN-N-10
Plasmid#55483PurposeLocalization: Membrane Associated Kinase, Excitation: 462, Emission: 492DepositorInsertFYN (FYN Human)
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)
Plasmid#128604PurposeExpresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsertsUseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mGreenLantern
Plasmid#164469PurposeConstitutive CAG-driven expression of mGreenLantern in animals using AAVDepositorInsertmGreenLantern
UseAAVTagsExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCAGAvailable sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only