We narrowed to 5,923 results for: pcDNA 3.1

1. eTC GFP ß-actin ΔZip

   Plasmid

   #27124

   Depositor

   Robert Singer

   Article

   Rodriguez et al J Cell Biol. 2006 Oct 9. 175(1):67-76.

   Insert

   beta-actin (ACTB Human)
   

   Use

   Tags

   EGFP and TC tag

   Expression

   Mammalian

   Mutation

   This construct lacks β-actin 3′UTR and therefore …

   Promoter

   Available since

   Jan. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-YL1

   Plasmid

   #15333

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Cai et al J Biol Chem. 2005 Apr 8. 280(14):13665-7

   Insert

   transcription factor-like 1 (VPS72 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

3. psgRNA2.02-PP7-ccdB

   Plasmid

   #99912

   Purpose

   Basic cloning vector for the expression of PP7 inserted sgRNA

   Depositor

   Yujie Sun

   Article

   Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOVC1_EcSSPB-nano_X

   Plasmid

   #131212

   Purpose

   Expresses EcSSPB-nano (Escherichia coli) followed by restriction sites

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   EcSSPB-nano
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. psgRNA2.02-MS2-ccdB

   Plasmid

   #99911

   Purpose

   Basic cloning vector for the expression of MS2 inserted sgRNA

   Depositor

   Yujie Sun

   Article

   Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. NSarQ27G-VMAn-FKBP

   Plasmid

   #70230

   Purpose

   Expresses N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

   Depositor

   Perry Howard

   Article

   Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

   Insert

   alpha Sarcin (N-terminal portion)
   

   Use

   Tags

   FK506- and rapamycin-binding protein (FKBP), Link…

   Expression

   Mammalian

   Mutation

   Changed Gln 27 to Gly

   Promoter

   CMV

   Available since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pFOX6-Tier1-PhCMV-TdTomato

   Plasmid

   #169515

   Purpose

   Tier-1 vector encoding PhCMV-driven bright-red fluorescent protein TdTomato (PhCMV-TdTomato-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven dimeric red fluorescent protein TdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmyc-GFP-TNRC6A-NLS-mut

   Plasmid

   #42000

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   R930A, R931A, R932A, R934A

   Promoter

   CMV

   Available since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. PA-RL-mCherry

   Plasmid

   #78861

   Purpose

   DULIP negative control (bait) to exclude unspecific background binding

   Depositor

   Erich Wanker

   Article

   Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

   Insert

   mCherry
   

   Use

   Expression vector

   Tags

   Protein A, Renilla luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVH72-Tier1-PhCMV-SEAP

    Plasmid

    #169560

    Purpose

    Tier-1 vector encoding PhCMV-driven SEAP expression containing a computationally optimized secretion peptide (PhCMV-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven human secreted placental alkaline phosphatase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. TRPML1-MYC

    Plasmid

    #18824

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML1 (MCOLN1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. OSCA1.2-PP-GFP

    Plasmid

    #135390

    Purpose

    Expresses OSCA1.2 in mammalian cells

    Depositor

    Ardem Patapoutian

    Article

    Jojoa-Cruz et al Elife. 2018 Nov 1;7. pii: 41845. doi: 10

    Insert

    OSCA1.2 (AT4G22120 Mustard Weed)
    

    Use

    Tags

    EGFP tag connected to the gene via a precision pr…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. hNCAM2

    Plasmid

    #84473

    Purpose

    expression of hNCAM2 in mammalian cells

    Depositor

    Vladimir Sytnyk

    Article

    Leshchyns'ka et al Nat Commun. 2015 Nov 27;6:8836. doi: 10.

    Insert

    NCAM2 (NCAM2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. HIPK1-myc-his

    Plasmid

    #1850

    Depositor

    Philip Leder

    Article

    Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

    Insert

    HIPK1 (Hipk1 Mouse)
    

    Use

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pAL189

    Plasmid

    #22278

    Purpose

    Expresses Intersectin DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    IntersectinDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cenp-B DBD INCENP mCherry

    Plasmid

    #45233

    Purpose

    Target INCENP to centromeres via CENP-B DNA binding domain (DBD)

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    Cenp-B-DBD INCENP-Δcen mCherry
    

    Use

    Tags

    DNA binding domain of CENP-B and mCherry

    Expression

    Mammalian

    Mutation

    CEN box deleted from INCENP (aa1-46)

    Promoter

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVH232-Tier1-PmPGK1-mRuby2

    Plasmid

    #169523

    Purpose

    Tier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven monomeric red fluorescent protein mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. aq-CFP

    Plasmid

    #36073

    Depositor

    Narasimhan Gautam

    Article

    Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

    Insert

    G protein subunit alpha q (Gnaq Mouse)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    CFP inserted between amino acid 124 and 125

    Promoter

    CMV

    Available since

    Feb. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pGas-miRFP670nano3

    Plasmid

    #184672

    Purpose

    G protein alpha-subunit internally tagged with miRFP670nano3

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    G protein alpha-s-miRFP670nano3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pmyc-GFP-TNRC6A-(1-1223)

    Plasmid

    #42013

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa1-1223

    Promoter

    CMV

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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