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pAAV-CWB-pre-eGRASP(p30)

Plasmid

#111597

Purpose

An AAV vector that expresses green pre-eGRASP(p30) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

green pre-eGRASP(p30)

Use

AAV

Promoter

CaMKIIa

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-NR5A1

Plasmid

#192915

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of NR5A1

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

NR5A1 (NR5A1 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS

Plasmid

#154774

Purpose

plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma PylRS for ochre suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Mutation

in PylT: UUA anticodon and G10A, G14U, G16U, C20U…

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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nesprin TSmod 25

Plasmid

#125555

Purpose

Nesprin-2G force sensor with cerulean-F25-venus TSmodule

Depositor

Daniel Conway

Article

Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

Insert

mini-nesprin-2G (Syne2 Mouse)

Tags

cerulean, venus

Expression

Mammalian

Mutation

TSmod changed from mTFP to cerulean, flageliform …

Promoter

CMV

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe RFP1-KRT5 hygro

Plasmid

#58493

Purpose

mammalian expression of mRFP1-tagged human KRT5

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

KRT5 (KRT5 Human)

Tags

mRFP1

Expression

Mammalian

Available Since

Aug. 19, 2014

Availability

Academic Institutions and Nonprofits only
pBabe DN-FOXO1-HA neo

Plasmid

#45814

Depositor

Kevin Janes

Article

Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

Insert

FOXO1 (FOXO1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

aa1-255

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-TGFBR2-wt_3'UTR

Plasmid

#31882

Depositor

Robert Blelloch

Article

Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

Insert

TGFBR2 wild-type 3'UTR (TGFBR2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP-RapR-FAK-YM

Plasmid

#25928

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

RapR-FAK (Ptk2 Mouse, Human)

Tags

EGFP

Expression

Mammalian

Mutation

This pEGFP-RapR-FAK-YM plasmid contains the FAK m…

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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pHR-iRFP670-CaaX

Plasmid

#170464

Purpose

Expresses iRFP670 fluorophore targeted to the plasma membrane using a prenylation motif

Depositor

John James

Article

Harris et al Mol Syst Biol. 2021 May;17(5):e10091. do

Insert

iRFP670-CaaX

Use

Lentiviral

Tags

CaaX

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX/PD-L1 WT

Plasmid

#121466

Purpose

Expression of GST-human PD-L1 in E. Coli

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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EasyFusion T2A-H2B-miRFP703

Plasmid

#113097

Purpose

General donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-miRFP703

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ASAP2s

Plasmid

#101276

Purpose

Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1

Depositor

Francois St-Pierre

Article

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Use

AAV

Expression

Mammalian

Promoter

hSyn (human synapsin)

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-PH(Akt2)

Plasmid

#86596

Purpose

Expresses mCherry-tagged PH(WT) domain of human Akt2

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2 PH domain (AKT2 Human)

Expression

Mammalian

Mutation

Amino acids 1 - 108

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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pERB221

Plasmid

#61502

Purpose

const. CenpB-GFP-Halo + mCherry-DHFR

Depositor

David Chenoweth, Michael Lampson

Article

Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

Inserts

CenpB-GFP-Halo
mCherry-DHFR

Tags

HaloTag and mCherry

Expression

Mammalian

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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mEmerald-Paxillin-22

Plasmid

#54219

Purpose

Localization: Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Paszek et al Nat Methods. 2012 Jul 1;9(8):825-7. doi:

Insert

Paxillin

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBRHDAd-TKVenus

Plasmid

#26422

Depositor

Kohnosuke Mitani

Article

Suzuki et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

HSV-TK, CS2-Venus

Use

Adenoviral

Expression

Mammalian

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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3XFlag DVL2 (∂361-736)

Plasmid

#24806

Depositor

Jeff Wrana

Article

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Insert

DVL2 (DVL2 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of aa 361-736

Available Since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pFUW-tetO-Cebpa

Plasmid

#139834

Purpose

doxycycline-inducible expression of mouse Cebpa in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Cebpa (Cebpa Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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SMA-Cherry

Plasmid

#110209

Purpose

Mapping of mesenchymal progenitor cells.

Depositor

David Rowe

Article

Grcevic et al Stem Cells. 2012 Feb;30(2):187-96. doi:

Insert

mCherry

Tags

None

Expression

Mammalian

Promoter

Smooth muscle alpha-actin and first intron

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti Dest BlastR R1-R2

Plasmid

#84574

Purpose

Gateway destination vector expressing Blasticidin resistance under a PGK promoter

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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