We narrowed to 19,001 results for: Tec

1. pHSB04X

   Plasmid

   #117258

   Purpose

   Genome editing for Lactobacillus brevis ATCC367

   Depositor

   Sheng Yang

   Article

   Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

   Insert

   Cas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019
   

   Use

   Other

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. EK0498 SFFV-mCherry-SGc(ACTB.s1)cSG-EGFP-(3xMS2) (FLP-IN)

   Plasmid

   #191182

   Purpose

   Expression of EACTB sensor in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:ACTB.s1:EGFP-3xMS2
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pH-AFID-3

   Plasmid

   #167361

   Purpose

   For inducing predictable deletions in rice plants

   Depositor

   Caixia Gao

   Article

   Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

   Insert

   A3A-Cas9-UDG-T2A-AP lyase
   

   Expression

   Plant

   Mutation

   WT

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCellFree_G03 CYP3A4

   Plasmid

   #67104

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CYP3A4 (CYP3A4 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9-mSA*

   Plasmid

   #182952

   Purpose

   Express SpCas9-mSA*

   Depositor

   Wen Xue

   Article

   Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

   Insert

   SpCas9-mSA*
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCpf1b

   Plasmid

   #122187

   Purpose

   Bacterial genome editing plasmid with kanamycin resistance marker and using sacB as a counter-selection marker

   Depositor

   Cheng-Cai Zhang

   Article

   Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. CP1041-ABEmax

   Plasmid

   #119808

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSpCas9 (CP1041)
   

   Expression

   Mammalian

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pR26 GFP Dest

   Plasmid

   #74283

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloning

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCfB6633

   Plasmid

   #106162

   Purpose

   EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6677 into Yarrowia lipolytica chromosomal location IntE_1, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Insert

   unknown
   

   Expression

   Yeast

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. BiP-mCherry

    Plasmid

    #62233

    Purpose

    Hamster BiP/GRP78/HSPA5 fused to mCherry. Useful for studying BiP mobility and trafficking in live cells.

    Depositor

    Erik Snapp

    Article

    Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

    Insert

    hamster BiP-mCherry-KDEL
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-ExRai-AKAR

    Plasmid

    #118405

    Purpose

    cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AKAR
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2+ GFP-2xESP3I-IRES-mCherry

    Plasmid

    #230996

    Purpose

    Protein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

    Type

    Empty backbone

    Tags

    AcGFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTlpA_mCherry_Txe/Axe

    Plasmid

    #229147

    Purpose

    Toxin-Antitoxin (Txe/Axe)

    Depositor

    Shrikrishnan Sankaran

    Article

    Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

    Insert

    mCherry, Txe/Axe
    

    Expression

    Bacterial

    Promoter

    PtlpA (mCherry), Txe/Axe (Native)

    Available Since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAB1619 hU6-BpiI-Cas13bt1-DR

    Plasmid

    #176314

    Purpose

    hU6-BpiI-Cas13bt1-DR (crRNA expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    Cas13bt1 crRNA direct repeat
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTK194

    Plasmid

    #59563

    Purpose

    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak mKate opal attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP XbaI attB1 Kozak mKate opal attB2 AscI
    

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. U6-spTRE3G-CMV-mTagBFP2

    Plasmid

    #102854

    Purpose

    A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    sp-TRE3G-sgRNA; mTagBFP2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAF-HdrB3

    Plasmid

    #184908

    Purpose

    carrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferridurans

    Depositor

    Radhakrishnan Mahadevan

    Article

    Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

    Insert

    Cas9-sgRNA-homology arms
    

    Use

    CRISPR; Broad-host-range

    Expression

    Bacterial

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST-hisMBP-AsCpf1-EC

    Plasmid

    #79007

    Purpose

    Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    AsCpf1
    

    Tags

    6xHis-MBP-NLS and NLS-HA

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMJA284= pSin-Pur-MCS-Puro

    Plasmid

    #116877

    Purpose

    Lentiviral transfer vector containing a multible cloning site

    Depositor

    Marcos Alcocer

    Article

    Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-Cas9-NRTH

    Plasmid

    #136925

    Purpose

    Expresses Cas9-NRTH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9-NRTH
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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