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pHR_Gal4UAS_Tbet_T2A_GFP_PGK_mCherry

Plasmid

#85431

Purpose

Lentiviral vector for Gal4 inducible Tbet T2A GFP and constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human Tbet

Use

Lentiviral

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pFH3.29_Cre

Plasmid

#205560

Purpose

Express Cre (without export tag)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Cre

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-RACK1

Plasmid

#41088

Depositor

Anna Huttenlocher

Article

Cox et al Mol Biol Cell. 2003 Feb;14(2):658-69.

Insert

Receptor for activated C kinase (RACK1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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FR_GFP

Plasmid

#31302

Depositor

Gerhart Ryffel

Article

Erdmann et al Biol Chem. 2007 Jan . 388(1):91-106.

Insert

green fluorescent protein

Expression

Mammalian

Available Since

Oct. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pSB-BbsI-sgRNA

Plasmid

#203359

Purpose

Contains BbsI cut sites for placement of user-specified sgRNA target sequences with Sleeping Beauty backbone

Depositor

Jonathan Brunger

Article

Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

Insert

BbsI Cut Sites

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pWS481

Plasmid

#123029

Purpose

LexA UAS

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (6x) [2a]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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PNCA-GFP-dYY1

Plasmid

#67791

Purpose

Use together with pCMV-intron and VSV-G to package MMLV reporter virus

Depositor

Stephen Goff

Article

Schlesinger et al Cell Rep. 2013 Jul 11;4(1):50-8. doi: 10

Insert

MMLV-LTR-GFP

Mutation

Deletion of LTR U3 YY1 binding domain

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
SOX2se-CPG-TV

Plasmid

#70155

Purpose

a targeting vector to target the endogenous Sox2 SE CPG island with Snrp-Tomato-polyA

Depositor

Rudolf Jaenisch

Article

Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

Insert

5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se

Use

Mouse Targeting and Synthetic Biology

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP(C1)-PP1gamma

Plasmid

#44225

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Article

Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

Insert

PP-1G (PPP1CC Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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CMVdel-dimericTomato-WASp(CRIB)

Plasmid

#191450

Purpose

Visualizing endogenous & active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WASp

Expression

Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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dimericTomato-1xwGBD

Plasmid

#176099

Purpose

Visualization of active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WAS (WAS Human)

Use

Lentiviral

Promoter

CMVdel

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMB80 (R26-CreER)

Plasmid

#12168

Depositor

Tyler Jacks

Article

McLaughlin et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

Rosa26 CreER^T2

Use

Cre/Lox

Expression

Mammalian

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry-RORbshRNA1

Plasmid

#73983

Purpose

RORb shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the mouse RORb gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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Multi-frame tag

Plasmid

#128610

Purpose

In the Multi-frame tag, HIV-1 frameshift sequence is upstream of FLAG epitopes in the 0 frame are separated from one another by SunTag epitopes in the -1 frame.

Depositor

Tim Stasevich

Article

Lyon et al Mol Cell. 2019 Jul 11;75(1):172-183.e9.

Insert

MF tag

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

Plasmid

#174529

Purpose

Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

Depositor

Yu-Hsuan Tsai

Article

Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

Inserts

PylS
PylT

Tags

Flag

Expression

Mammalian

Promoter

U6

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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cmv-sfGFP-GB1-scAB-ubc-nls-pcp-10xSunTag(SRv1.1-2P)

Plasmid

#185801

Purpose

one plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.1

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

cmv-sfGFP-GB1-scAB--ubc-nls-pcp-10xSunTag

Expression

Mammalian

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro

Plasmid

#136262

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 8 repeats

Use

Tol2 transposase

Tags

mNeonGreen-NLS

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_class_I_2

Plasmid

#164987

Purpose

Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA class I

Use

CRISPR

Expression

Mammalian

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pPBpuro-ERK-KTR-mCherry

Plasmid

#214829

Purpose

Encoding a fluorescent reporter for ERK activity

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

ERK KTR-mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CAG promoter

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pX330GFP-hU6-gRNA-hSpCas9

Plasmid

#128385

Purpose

GFP positive gRNA/hSpCas9 construct

Depositor

Budd A. Tucker

Article

Giacalone et al Curr Protoc Stem Cell Biol. 2018 Feb 28;

Insert

GFP, gRNA and hSpCas9

Use

CRISPR

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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