We narrowed to 14,596 results for: sta
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Plasmid#82337PurposeProvides a C-terminal macro domain and 6xHis tag for expression in E. coliDepositorInsertSPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
Tags6xHis non-cleavable and macro domain of human his…ExpressionBacterialMutationTruncated protein, amino acids 1-178Available SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3 2198
Plasmid#49347PurposeThis is actually plasmid pGL3 2185 from the associated publication, which contains a shorter Bmp2 promoter sequence. also contains luciferase reporter.DepositorInsertBmp2 promoter (shorter sequence) (BMP2 Human)
UseLuciferaseTagsluciferasePromoterBmp2 promoterAvailable SinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
PSuperPuroRetro-1660s(hPot1)
Plasmid#11136DepositorInsertRNAi against Human Protection of Telomeres 1 (control) (POT1 Human)
UseRNAi and RetroviralExpressionMammalianAvailable SinceMarch 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
GABPA_pcDNA6.2/EmGFP-Bsd
Plasmid#176970PurposeMammalian expression vector encoding GABPA and EmGFP-BsdDepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
MRAP_pcDNA6.2/EmGFP-Bsd
Plasmid#176937PurposeMammalian expression vector encoding MRAP and EmGFP-BsdDepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-KSENB-Nup133-mut
Plasmid#87339PurposeTo express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNADepositorInsertNUP133 (NUP133 Human)
TagsEGFPExpressionMammalianMutationshRNA resistant changes; deletion of Nup133 AA1-7PromoterCMVAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-Mm2
Plasmid#197100PurposeTo express the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA and allow UAG codon to be translated into these derivatiDepositorInsertPylRS variant, Pyl tRNA, kanamycin resistance gene
ExpressionBacterialMutationLys61, Glu131, Ala306, Phe384, Glu444 (PylRS)Available SinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-Munc18-1wt
Plasmid#135550PurposeExpress rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertMunc18-1wt
Tags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR221 UBR5 ∆PABC
Plasmid#81064PurposeGateway entry vector encoding full length UBR5 Y2402A/K2415A (∆PABC)DepositorInsertUbiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆PABC (UBR5 Human)
UseCloningMutationY2402A and K2415A (bp t7204g, a7205c, t7206c, a72…PromoterN/AAvailable SinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPHAGE_DLD-S456A-C-TAP
Plasmid#83479PurposeMammalian expression of the proteolysis-deficient mutant DLD-S456A.DepositorAvailable SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRJPaph-lacZYA
Plasmid#67037PurposePlasmid for Paph-lacZYA integration downstream of B. diazoefficiens (B. japonicum) scoIDepositorInsertsB. diazoefficiens DNA from scoI downstream region (comprising blr1132')
lacZYA
UseConjugative bacterial vectorAvailable SinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Halotag-loxP-P2A-dTKneo-pA-loxP
Plasmid#139485PurposePCR template for Halo-Tag gene knock-in donor vectorDepositorInsertHaloTag-loxP-P2A-dTKneo-pA-loxP
TagsHaloTagExpressionMammalianPromoterCAG promoterAvailable SinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mEGFP-loxP-P2A-dTKneo-pA-loxP
Plasmid#139484PurposePCR template for mEGFP gene knock-in donor vectorDepositorInsertmEGFP-loxP-P2A-dTKneo-pA-loxP
TagsmEGFPExpressionMammalianPromoterCAG promoterAvailable SinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF1a (S668A)
Plasmid#49463PurposeHA-tagged HIF-1a Ser668 resuide mutated to AlaDepositorInsertHypoxia inducible factor 1 alpha (HIF1A Human)
TagsHAExpressionMammalianMutationSerine 668 to AlaninePromoterT7Available SinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRQ-hRasV12
Plasmid#18113DepositorInserth-RasV12G (HRAS Human)
UseRetroviralExpressionMammalianMutationG12V, constitutively activeAvailable SinceFeb. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
GNAS_pcDNA6.2/EmGFP-Bsd
Plasmid#176976PurposeMammalian expression vector encoding GNAS and EmGFP-BsdDepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCA-T-intron(Neo)-G(LNL)
Plasmid#36908DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Neo
ExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available SinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
delCMV-mCherry-3x-p67phox-GBD
Plasmid#214141PurposeRac activity sensorDepositorInsert3xp67phox-GBD (NCF2 Human)
TagsmCherryExpressionMammalianMutationOnly the GTPase binding domain (GBD) is includedPromoterdelCMVAvailable SinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa(0.4)-PdCO-mScarlet-WPRE
Plasmid#198508PurposeExpresses optimized PdCO in frame with mScarlet under control of minimal CamKIIa promotor.DepositorInsertPdCO
UseAAVTagsRho1D4 and mScarletExpressionMammalianPromoterCaMKIIα minimal promotor (0.4kb)Available SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-hNPM
Plasmid#181887PurposeSoluble light-dependent effector protein (nucleophosmin) for optodroplet formation or recruitment to CIBN localizersDepositorInsertPHR-mCherry-hNPM (NPM1 Human, Mustard Weed)
TagsPHR-mCherryExpressionMammalianPromoterCMVAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only