We narrowed to 8,515 results for: sgrna

1. BPK1520

   Plasmid

   #65777

   Purpose

   Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   SpCas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXAT2

   Plasmid

   #80494

   Purpose

   AAVS1 sgRNA expression vector

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   AAVS1 sgRNA-T2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLC-EGFP-RIP3

   Plasmid

   #75165

   Purpose

   LentiCRISPR-EGFP with sgRNA targeting human RIP3

   Depositor

   Beat Bornhauser

   Article

   McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

   Insert

   RIP3 sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. QPM-sgR (pTaU3)

   Plasmid

   #140448

   Purpose

   For sgRNA expression in monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

   Insert

   TaU3-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   TaU3

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3015b

   Plasmid

   #209317

   Purpose

   Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.

   Depositor

   Thomas Hooven

   Article

   Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1

   Insert

   Group B Streptococcus-compatible single guide RNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Xyl/tet promoter that is constitutively active

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRDA_526

   Plasmid

   #184859

   Purpose

   Lentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.

   Depositor

   Arlene Sharpe

   Article

   Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

   Insert

   sgRNA cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLGR002 LGR Cas9 guide vector

   Plasmid

   #188320

   Purpose

   Universal backbone for CRISPR/Cas9 sgRNA expression

   Depositor

   Laralynne Przybyla

   Article

   Heo et al Genome Biol. 2024 Jan 19;25(1):25. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   PuroR-T2A-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   pU6 5'CMV and EF1-alpha

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2-nCas9n-nanos 3’UTR

   Plasmid

   #62542

   Purpose

   expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

   Depositor

   Antonio Giraldez

   Article

   Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

   Insert

   nanos 3' UTR (nanos3 Zebrafish)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   3' UTR only

   Promoter

   SP6

   Available since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. LRG2.1_Neo

   Plasmid

   #125593

   Purpose

   Lentiviral expression of sgRNA with GFP and neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter for sgRNA expression and EFS promoter…

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-CENPA

    Plasmid

    #227282

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of CENPA (CENPA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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