167,318 results

1. pSpCas9_T2A_iRFP670 sgBFP

   Plasmid

   #192478

   Purpose

   All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Tags

   T2A iRFP670

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP

   Plasmid

   #69908

   Purpose

   Expresses a GFP circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1, GFP (ZKSCAN1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-6P1-hPXR-LBD

   Plasmid

   #67769

   Purpose

   Expression GST-hPXR-LBD in bacteria

   Depositor

   Sridhar Mani

   Article

   Li et al J Biol Chem. 2013 May 10;288(19):13655-6

   Insert

   human pregnane X receptor (PXR) ligand binding domain (NR1I2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Contains amino acids 107 to 434 of human PXR

   Promoter

   Tac

   Available Since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-SARS2-Spike

   Plasmid

   #145032

   Purpose

   Express SARS-CoV-2 spike protein with C9 tag at C-terminal in mammalian cells

   Depositor

   Fang Li

   Article

   Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

   Insert

   SARS-CoV-2 Spike (S Synthetic)
   

   Tags

   C9

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pMEH303 NLS dTomato 6x high affinity MS2 loops

   Plasmid

   #168215

   Purpose

   Mammalian expression of nuclear-localized dTomato with 6 high affinity MS2 loops

   Depositor

   Joshua Leonard

   Article

   Hung et al J Extracell Vesicles. 2016 May 13;5:3102

   Insert

   NLS dTomato
   

   Use

   Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-mNINJ1-GFP

   Plasmid

   #208775

   Purpose

   Inducibly expresses mouse NINJ1 tagged with GFP

   Depositor

   Petr Broz

   Article

   Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

   Insert

   mNINJ1 (Ninj1 Mouse)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Aug. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-SFFV-FLAG-SQSTM1

   Plasmid

   #223715

   Purpose

   Expresses Flag-tagged SQSTM1

   Depositor

   Benjamin Wolozin

   Article

   Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

   Insert

   SQSTM1 (SQSTM1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pMK344 (Hygro-P2A-mAID)

   Plasmid

   #121178

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Hygro-P2A-mAID
   

   Use

   CRISPR

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT2/CMV-Luc/PGK-SB100X

   Plasmid

   #192869

   Purpose

   Carries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.

   Depositor

   David Largaespada, Maria Castro

   Article

   Calinescu et al J Vis Exp. 2015 Feb 22;(96). doi: 10.379

   Inserts

   luc
   SB100X
   

   Expression

   Mammalian

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHIV-ODD-EGFP-IRES-dTomato

    Plasmid

    #87218

    Purpose

    lentiviral expression of ODD-EGFP

    Depositor

    Violaine Sée

    Article

    Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

    Insert

    HIF-1a ODD domain (HIF1A Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    ODD domain

    Promoter

    EF1

    Available Since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDisplay-GRAB-rDA3m-IRES-EGFP-CAAX

    Plasmid

    #208696

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m and a membrane-localized EGFP in mammalian cells

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA3m
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpCas9-NRCH-ABE8e(V106W)

    Plasmid

    #198556

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8e(V106W)-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-ABE8e(V106W)
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVZmSin3B

    Plasmid

    #30453

    Depositor

    Bob Eisenman

    Article

    Ayer et al Cell 1995 Mar 10;80(5):767-76.

    Insert

    Sin3B (Sin3b Mouse)
    

    Expression

    Mammalian

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pNO286-3

    Plasmid

    #107746

    Purpose

    Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)

    Depositor

    Jeffrey Tabor

    Article

    Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cb

    Inserts

    CcaS
    Ho1
    PcyA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Deleted AA 223 and 512

    Promoter

    J23106 and J23108

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV-mEGFP(A206K)-paCaMKII

    Plasmid

    #165438

    Purpose

    Expresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII
    

    Tags

    mEGFP(A206K)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti6.2_3xFLAG_V5_ mmIfih1_V5

    Plasmid

    #160110

    Purpose

    Expresses murine Ifih1 in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Ifih1 (Ifih1 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFLAG, V5 and V5

    Expression

    Mammalian

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas9-Geminin

    Plasmid

    #109401

    Purpose

    Expresses Cas9 fusion to Geminin degron domain

    Depositor

    Jean-Paul Concordet

    Article

    Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

    Insert

    Geminin (GMNN Human)
    

    Mutation

    Geminin degron fragment

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCherry.90 alpha

    Plasmid

    #108222

    Purpose

    Mammalian expression vector for mCherry fusion to human Hsp90 alpha

    Depositor

    Didier Picard

    Article

    Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

    Insert

    mCherry-Hsp90a (HSP90AA1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME18S-HismSin3A

    Plasmid

    #30454

    Depositor

    Bob Eisenman

    Insert

    Sin3A (Sin3a Mouse)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pENN.AAV.hSyn.Cre.WPRE.hGH (AAV8)

    Viral Prep

    #105553-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA. hSyn-driven Cre expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    Syn

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only