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We narrowed to 9,772 results for: Tec;

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Showing: 4701 - 4720 of 9772 results

  4. pCOCK CM-R05

    Plasmid
    #46568
    Purpose
    Mammalian expression plasmid with EGFP expressed from synthetic promoter (random mutagenesis of CMVwt 3' end).
    Depositor
    Clifford Wang
    Article
    Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8.
    Insert
    CMV mutant promoter
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    Mutant version of CMV promoter (refer to Table 1 …
    Available Since
    Sept. 23, 2013
    Availability
    Academic Institutions and Nonprofits only

  5. pCIBN-LacI

    Plasmid
    #103814
    Purpose
    BLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-LacI (CIB1, lacI Mustard Weed, E. coli)
    Expression
    Mammalian
    Mutation
    LacI: C19T (silent, L7L), T264C (silent, A88A), C…
    Promoter
    CMV
    Available Since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103814-plasmid-map-sequence-id-199324

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  6. pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag

    Plasmid
    #216695
    Purpose
    Synchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)
    Depositor
    Franck Perez
    Article
    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s
    Inserts
    FIREmate-KDEL
    ManII-SBP-mApple-FIREtag (Man2a1 Mouse)
    Tags
    FIREtag, Streptavidin Binding Protein (SBP), and …
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV
    Available Since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216695-plasmid-map-sequence-id-429706

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  7. pcDNA3.1 Flag YFP hNKCC1 HA-ECL4 (NT935)

    Plasmid
    #49065
    Purpose
    Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #4 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    2xHA epitope in ECL4 inserted at aa574 in hNKCC1 …
    Promoter
    CMV
    Available Since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49065-plasmid-map-sequence-id-72190

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  8. pcDNA3.1 Flag YFP hNKCC1 HA-ECL2 (NT931)

    Plasmid
    #49063
    Purpose
    Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #2 and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    2xHA epitope in ECL2 inserted at aa398 in hNKCC1 …
    Promoter
    CMV
    Available Since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49063-plasmid-map-sequence-id-72185

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  10. pLK06Hpp-Fab CR3009

    Plasmid
    #239861
    Purpose
    Plasmid for simultaneous Fab and GFP expression from separate Lac promoters for online monitoring of induction with a GFP sensor station. Fab is expressed as a fusion with alkaline phosphatase.
    Depositor
    Urpo Lamminmäki
    Article
    Korkiakoski et al Protein Expr Purif. 2025 Aug 28:106808.
    Inserts
    Fab (antigen binding fragment) anti-nucleocapsid protein of SARS-COV viruses 1 and 2 (N, N Human)
    GFP with F64L, S65T, Q80R, M153T and V163A mutations
    Tags
    Hexahistidine tag and bacterial alkaline phosphat…
    Expression
    Bacterial
    Mutation
    F64L, S65T, Q80R, M153T and V163A
    Promoter
    Lac promoter
    Available Since
    Sept. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239861-plasmid-map-sequence-id-485412

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  11. pCDNA3.1-nAChr-beta3-YFP

    Plasmid
    #50484
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    YFP fusion in M3-M4 loop (after residue P379). O…
    Promoter
    CMV
    Available Since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pZH_tdPP7cp_mCherry

    Plasmid
    #118267
    Purpose
    Low-noise, tetracycline inducible expression of tdPP7cp-mCherry
    Depositor
    Zach Hensel
    Insert
    tdPP7cp-mCherry
    Expression
    Bacterial
    Promoter
    pLtetO-1
    Available Since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    118267-plasmid-map-sequence-id-229509

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  15. CytERM_3xmTurquoise2

    Plasmid
    #112958
    Purpose
    In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)
    Depositor
    Dorus Gadella
    Insert
    CytERM
    Tags
    3x mTurquoise2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    112958-plasmid-map-sequence-id-220333

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  20. mCherry-myosinRLC

    Plasmid
    #105761
    Purpose
    Red NMMII phosphorylation sensor
    Depositor
    Megan Rizzo
    Insert
    mCherry-RLC
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    105761-plasmid-map-sequence-id-205137

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Showing: 4701 - 4720 of 9772 results