Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 8,727 results for: pcDNA3

Showing: 4701 - 4720 of 8727 results

Results per page:

Sort results by:

pcDNA3HA-AMOT p130∆PPxY1(E104K/E105K)

Plasmid

#171130

Purpose

expresses AMOT(E104K/E105K) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

Angiomotin p130 E104K/E105K (AMOT Human)

Tags

2xHA

Expression

Mammalian

Mutation

changed glutamic acid residues 104 and 105 to lys…

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 LIC 6A CARD8-FLAG R394E + S297A

Plasmid

#169955

Purpose

Mammalian expression of CARD8-FLAG with a site III mutation (R394E) with an additional autoprocessing mutation (S297A)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S297A, R394E

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 LIC 6A CARD8-FLAG F405G + S297A

Plasmid

#169957

Purpose

Mammalian expression of CARD8-FLAG with a site III mutation (F405G) with an additional autoprocessing mutation (S297A)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S297A, F405G

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG L368G

Plasmid

#169959

Purpose

Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG and Ub

Expression

Mammalian

Mutation

L368G

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG R394E

Plasmid

#169961

Purpose

Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG and Ub

Expression

Mammalian

Mutation

R394E

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F405G

Plasmid

#170119

Purpose

Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed).

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG and Ub

Expression

Mammalian

Mutation

F405G

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 LIC 6A CARD8-FLAG E274R + S297A

Plasmid

#169951

Purpose

Mammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R) with an additional autoprocessing mutation (S297A)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E274R, S297A

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 LIC 6A FLAG-TEV-DPP9S RK96EE

Plasmid

#166797

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. RK96EE mutant is partial loss of CARD8 binding.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

FLAG-TEV

Expression

Mammalian

Mutation

RK96EE

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL102EE

Plasmid

#166799

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. LL102EE mutant is partial loss of CARD8 binding.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

FLAG-TEV

Expression

Mammalian

Mutation

LL102EE

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 LIC 6A FLAG-TEV-DPP9S D808N

Plasmid

#166802

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. The D808N mutant is catalytically dead.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

FLAG-TEV

Expression

Mammalian

Mutation

D808N

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 6B CARD8 UPA-CARD-mCherry R495E

Plasmid

#164016

Purpose

Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

mCherry

Expression

Mammalian

Mutation

R495E

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 6B CARD8 UPA-CARD-mCherry Y527A

Plasmid

#164018

Purpose

Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Y527A

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG W1460A

Plasmid

#163989

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

W1460A

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG Y1445A

Plasmid

#163987

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Y1445A

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG R1427E

Plasmid

#163986

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

R1427E

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1414R

Plasmid

#163985

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E1414R

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1411R

Plasmid

#163984

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E1411R

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG D1401R

Plasmid

#163983

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

R1401R

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1397R

Plasmid

#163982

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E1397R

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG R1392E

Plasmid

#163981

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

R1392E

Available Since

Jan. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 4701 - 4720 of 8727 results

Results per page:

Sort results by: