167,785 results

1. pSEP-LAMP1-RFP

   Plasmid

   #167970

   Purpose

   Expresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFP

   Depositor

   Juan Bonifacino

   Article

   Farias et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Insert

   LAMP1 (Lamp1 Rat)
   

   Tags

   SEP and mRFP

   Expression

   Mammalian

   Mutation

   D50E

   Promoter

   CMV

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0799

   Plasmid

   #141705

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ATF3 (ATF3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ132B2.0

   Plasmid

   #99888

   Purpose

   Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   AtU3

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXs-IP GFP-NBR1

   Plasmid

   #38283

   Depositor

   Noboru Mizushima

   Article

   Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

   Insert

   Next to BRCA1 gene 1 protein (NBR1 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-EF1a-jGCaMP8s-WPRE (AAV1)

   Viral Prep

   #176758-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from AAV-EF1a-jGCaMP8s-WPRE (#176758). In addition to the viral particles, you will also receive purified AAV-EF1a-jGCaMP8s-WPRE plasmid DNA. EF1a-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Loren Looger

   Promoter

   EF1a

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

6. pCAG-ChrimsonR-tdT

   Plasmid

   #59169

   Purpose

   Expression vector for ChrimsonR-tdTomato

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-tdTomato
   

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   K176R

   Promoter

   CAG

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro

   Plasmid

   #188768

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Promoter

   SFFV

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB533_15F11-sfGFP

   Plasmid

   #167526

   Purpose

   H4K20me1-mintbody expression vector for mammalian cells

   Depositor

   Hiroshi Kimura

   Article

   Sato et al J Mol Biol. 2016 Oct 9;428(20):3885-3902

   Insert

   15F11-scFv
   

   Tags

   sfGFP

   Expression

   Mammalian

   Available Since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLViP-LifeAct-iRFP670

   Plasmid

   #229699

   Purpose

   Lentiviral expression of LifeAct-iRFP670 in mammalian cells

   Depositor

   Alpha Yap

   Article

   Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

   Insert

   Synthetic construct LifeAct-mTurquoise gene
   

   Use

   Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-AAV-syn-jGCaMP7s-WPRE (AAV9)

    Viral Prep

    #104487-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pGP-AAV-syn-jGCaMP7s-WPRE (#104487). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven GCaMP7s calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Promoter

    Syn

    Available Since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only