We narrowed to 25,285 results for: crispr
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Plasmid#101920PurposeThe dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.DepositorInsertdCas9-TET1CD Mut
UseLentiviralExpressionMammalianMutationMutated TET1 catalytic domainAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pspCas9-3exo-Tetra-com-CLCN5-sp-g1
Plasmid#176236PurposePlasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.DepositorInsertspCas9 and polymerase exo domain fusion (CLCN5 Human)
ExpressionBacterialAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-5Exo-tetra-com-hCLCN5-sp-g1
Plasmid#176237PurposePlasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.DepositorInsertspCas9 and polymerase exo domain fusion (CLCN5 Human)
ExpressionBacterialAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
2a-copGFP(+HA)
Plasmid#83806PurposeExpress copGFP reporter for HDR-induced knock-inDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcopGFP
UseHomologous recombination for gapdh lociAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330-gRNA
Plasmid#158973PurposeA human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA3
ExpressionMammalianPromoterU6Available sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJMP1237
Plasmid#119262Purposefor cloning new sgRNAsDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ACBE-Q
Plasmid#204608PurposeMammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-QDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertbpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
UseCRISPRTags6*HisExpressionMammalianMutationTadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …Available sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGTag-TagRFP-SV40
Plasmid#117807PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTagRFP
UseSynthetic BiologyAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pWN_U6-B2M-miniGag-Cas9
Plasmid#228958PurposeminiEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertminiGag-Cas9
UseCRISPRExpressionMammalianAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-xF2X-P2A-Puro
Plasmid#110873PurposeLentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)DepositorInsertxFNLS-2X(3.7)
UseLentiviralTags3X FLAGMutationD10A, A262T, R324L, S409I, E480K, E543D, M694I, E…PromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
dCas9-FOG1[N+C]
Plasmid#100085PurposedCas9 fused to two FOG1 peptide [aa 1-45] , one at the N-terminus and one at the C-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFOG1(aa 1-45) (ZFPM1 Human)
UseCRISPRTags3XFLag-NLS-FOG1-dCas9-FOG1-NLSExpressionMammalianPromoterCMVAvailable sinceOct. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-24:MYH10-mEGFP
Plasmid#87428PurposeHomology arms and linker-mEGFP sequence for N-terminus tagging of human MYH10DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMYH10 Homology Arms with mEGFP-linker (MYH10 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available sinceMay 2, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
ABE8.17-m
Plasmid#136298Purposeexpresses ABE8.17-m in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertABE8.17-m
TagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…Available sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AICSDP-82:SON-mEGFP
Plasmid#133964PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human SONDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSON Homology Arms with mEGFP-linker (SON Human)
UseCRISPRTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available sinceNov. 22, 2019AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pspCas9-Exo-tetra-com-hCLCN5-sp-g1
Plasmid#176238PurposePlasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.DepositorInsertspCas9 and polymerase exo domain fusion (CLCN5 Human)
ExpressionBacterialAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-dCasRx-RBM25-IRES-BleoR
Plasmid#221002PurposeStable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCasRx-RBM25-IRES-BleoR
UseCRISPRTagsNLS and NLS-HAExpressionMammalianPromoterCAGAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA
Plasmid#158972PurposeVector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neuronsDepositorInsertdSaCas9-VP64
UseAAV and CRISPRExpressionMammalianPromoterpMecp2Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-CACNA1A KI
Plasmid#131480PurposeEndogenous tagging of CaV2.1, P/Q: N-terminal (amino acid position: G10)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR 2xU6:gRNA, mitfa:Cas9
Plasmid#118844PurposeTargets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytesDepositorTypeEmpty backboneUseCRISPR; Tol2Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDIRECT_21A
Plasmid#91128PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:hpt IIDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRExpressionPlantAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only