We narrowed to 14,684 results for: NTS;

1. pICH47742:: pRPS5a-PE2max-NC::tHSP

   Plasmid

   #227694

   Purpose

   Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Insert

   PE2max-NC being driven by pRPS5a and terminated by tHSP
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   pRPS5a

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNP568

   Plasmid

   #222725

   Purpose

   pNP555 - IS621 RE-LE TBL-WT, DBL-WT bRNA in cis

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 bridge RNA
   

   Tags

   sfGFP

   Expression

   Bacterial

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNTI935

   Plasmid

   #216638

   Purpose

   cpTurboID-PM (V5) Plasma membrane-localized cpTurboID, mammalian expression and lentivirus production

   Depositor

   Nicholas Ingolia

   Article

   Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

   Insert

   cpTurboID-PM
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. BPK3082

   Plasmid

   #78742

   Purpose

   Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   LbCpf1 crRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNP1723

   Plasmid

   #222727

   Purpose

   sfGFP-ON BsaI Golden Gate (GG) Landing Pad for LD-RD and J23119-BsaI GG Landing Pad for bRNA (bRNA in trans expression)

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Type

   Empty backbone

   Tags

   sfGFP

   Expression

   Bacterial

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSP1830

   Plasmid

   #70708

   Purpose

   Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Insert

   mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Mammalian

   Mutation

   E782K, N968K and R1015H in SaCas9

   Promoter

   CAG

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. BPK3079

   Plasmid

   #78741

   Purpose

   Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   AsCpf1 crRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNP2157

   Plasmid

   #222732

   Purpose

   pNP1950 - J23119-IS621 bRNA TBL-WT, DBL-WT, HDV; T7-IS621 Recombinase

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 Helper (WT)
   

   Expression

   Bacterial

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNP1047

   Plasmid

   #222726

   Purpose

   pNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cis

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 bridge RNA
   

   Tags

   sfGFP

   Expression

   Bacterial

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNP2270

    Plasmid

    #222733

    Purpose

    pNP1950 - J23119-IS621 bRNA1 TBL-lacZ-1 (Extended RTG), DBL-WT, HDV; T7-IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 Helper (lacZ)
    

    Expression

    Bacterial

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNTI934

    Plasmid

    #216637

    Purpose

    Cal-ID-PM (V5) Plasma membrane-localized Cal-ID, mammalian expression and lentivirus production

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    Cal-ID-PM
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHis APEX2-eIF4A1

    Plasmid

    #129645

    Purpose

    Express APEX2 fusion to DEAD-box translation factor eIF4A1 in bacteria

    Depositor

    Nicholas Ingolia

    Article

    Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

    Insert

    eukaryotic translation initiation factor 4A1 (EIF4A1 Human)
    

    Tags

    6xHis and APEX2

    Expression

    Bacterial

    Available Since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNTI821

    Plasmid

    #216630

    Purpose

    Tet-inducible Cal-ID (V5) for Sleeping Beauty transposon system

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    Cal-ID
    

    Use

    Sleeping beauty transposon system

    Tags

    V5

    Expression

    Mammalian

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SQT1665

    Plasmid

    #78744

    Purpose

    Human expression plasmid for LbCpf1-NLS-3xHA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SQT1659

    Plasmid

    #78743

    Purpose

    Human expression plasmid for AsCpf1-NLS-3xHA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    mammalian codon-optimized Cpf1-NLS-3xHA
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNTI822

    Plasmid

    #216631

    Purpose

    Tet-inducible cpTurboID (V5) for Sleeping Beauty transposon system

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    cpTurboID
    

    Use

    Sleeping beauty transposon system

    Tags

    V5

    Expression

    Mammalian

    Mutation

    L120P

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. BPK2139

    Plasmid

    #65776

    Purpose

    Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNTI827

    Plasmid

    #216635

    Purpose

    Cal-ID-ERM (V5) ER membrane-localized Cal-ID, mammalian expression and lentivirus production

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    Cal-ID-ERM
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC57-HBB-nLuc

    Plasmid

    #175432

    Purpose

    Used to generate IVT RNA for translation extracts

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    HBB-nLuc
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC Ad VAI

    Plasmid

    #175518

    Purpose

    For mammalian expression of adenovirus VAI RNA

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    Ad VAI RNA
    

    Use

    Adenoviral

    Promoter

    lac promoter

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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