We narrowed to 15,157 results for: pET

1. UMG-SP-3_pET29b

   Plasmid

   #215435

   Purpose

   Expression construct for UMG-SP-3 gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   UMG-SP-3
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET24b-Amg2

   Plasmid

   #199434

   Purpose

   expresses Amg2 in bacterial cells

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   Amg2
   

   Tags

   his tag

   Expression

   Bacterial

   Mutation

   L159K mutation

   Promoter

   T7

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET14-GFP15_Encap

   Plasmid

   #86407

   Purpose

   Expresses sfGFP tagged with a 15 amino acid CLP and encapsulin as an operon

   Depositor

   David Savage

   Article

   Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

   Inserts

   sfGFP with a 15aa CLP tag
   Encapsulin
   

   Tags

   6x Histidine

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET3a-Ub-K63R

   Plasmid

   #18898

   Depositor

   Cecile Pickart

   Article

   Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

   Insert

   Ubiquitin-K63R (UBC Human)
   

   Expression

   Bacterial

   Mutation

   Lysine 63 is replaced with a Argnine residue

   Available Since

   Sept. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET22b-ELP[V]60

   Plasmid

   #219406

   Purpose

   (VPGVG)60

   Depositor

   Danielle Mai

   Article

   Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

   Insert

   ELP[V]60
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-IG-mSA

   Plasmid

   #80706

   Purpose

   Expresses monomeric streptavidin (mSA) in bacterial cells

   Depositor

   Matthieu Sainlos

   Article

   Chamma et al Nat Commun. 2016 Mar 16;7:10773. doi: 10

   Insert

   Monomeric streptavidin
   

   Tags

   10xHis tag and TEV site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-cMSP1E3D1

   Plasmid

   #199772

   Purpose

   Expresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Daniilidis et al J Mol Biol. 2022 Dec 30;434(24):167861.

   Insert

   cMSP1E3D1
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   Extended cMSP1D1; external His-Tags flanking the …

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET22b-csgA

   Plasmid

   #89581

   Purpose

   Express csgA curli protein with GS linker and Histaq

   Depositor

   Urartu Seker

   Article

   Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

   Insert

   csgA (csgA )
   

   Tags

   3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…

   Expression

   Bacterial

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-SET

   Plasmid

   #234708

   Purpose

   expresses His-tagged human SET in E coli

   Depositor

   Lienhard Schmitz

   Article

   Seibert et al J Cell Biol. 2019 Apr 1;218(4):1164-1181

   Insert

   SET (SET Human)
   

   Tags

   (His)6

   Expression

   Bacterial

   Promoter

   lacI

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET24b-BG1.3

    Plasmid

    #199437

    Purpose

    expresses BAmGreen1.3 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMGreen1.3
    

    Tags

    his tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET11a-hRanGAP1

    Plasmid

    #50941

    Purpose

    expression of full length untagged human RanGAP1 in E.coli

    Depositor

    Frauke Melchior

    Article

    Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

    Insert

    RanGTPase activating protein 1 (RANGAP1 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-CPDSalI

    Plasmid

    #38252

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET29a-hNABP1

    Plasmid

    #128306

    Purpose

    Express hNABP1 (a.k.a hSSB2 or SOSSB2) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    Human nucleic acid-binding protein 1 (hNABP1) (NABP1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETDuet-TFIIE

    Plasmid

    #171083

    Purpose

    Co-expresses human TFIIE. The resulting plasmid was used to generate a single expression construct encoding 2 subunits, with His-tag on TF2E1.Originally from MN Gonzalez, was submitted with permission

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Inserts

    TF2E1 (GTF2E1 Human)
    TF2E2 (GTF2E2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-H12TEV

    Plasmid

    #208635

    Purpose

    for E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Tags

    6xhis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28_6xHis-PARP1delZn2

    Plasmid

    #173935

    Purpose

    Bacterial expression of PARP1 lacking Zn2 domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn2 (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 97-213 (V762A)

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-HsSENP3

    Plasmid

    #71467

    Purpose

    Encodes human full length SENP3. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 3 (SENP3 Human)
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28-MKH8SUMO

    Plasmid

    #79526

    Purpose

    Bacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Tags

    8xHis-Thrombin-SUMO-TEV and TEV cleavage site

    Expression

    Bacterial

    Available Since

    June 16, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pET15-ZmPYK

    Plasmid

    #220230

    Purpose

    Inducible expression of Zymomonas mobilis pyruvate kinase

    Depositor

    Aron Fenton, Liskin Swint-Kruse

    Article

    Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

    Insert

    Zymomonas mobilis pyruvate kinase (pyk, ZMO0152 Zymomonas mobilis)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCEP4YPet-MAMM

    Plasmid

    #14032

    Depositor

    Patrick Daugherty

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

    Insert

    YPet
    

    Expression

    Mammalian

    Mutation

    Mammalian optimized YPet

    Available Since

    Feb. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET151-GyrB

    Plasmid

    #216233

    Purpose

    S. aureus USA300 GyrB Codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET21a-C2-SNAP

    Plasmid

    #208769

    Purpose

    To express C2-SNAP in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2-SNAP (Mfge8 Mouse)
    

    Tags

    SNAP-tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. Ypet-PBD

    Plasmid

    #105290

    Purpose

    To measure Rac or Cdc42 activity by binding and FRET analysis

    Depositor

    Kenneth Yamada

    Article

    Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

    Insert

    Pak1 (PAK1 Human)
    

    Tags

    Ypet

    Expression

    Mammalian

    Mutation

    aa encoding 65-150

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET29b-pduCDE

    Plasmid

    #110138

    Purpose

    Expresses B12-dependent propanediol dehydratase in E. coli BL21(DE3)

    Depositor

    Emily Balskus

    Article

    Levin et al Biochemistry. 2018 Mar 16. doi: 10.1021/

    Insert

    pduCDE (pduE, pduD, pduC Klebsiella oxytoca ATCC 8724)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET28a(+)::NL3F10H

    Plasmid

    #141290

    Purpose

    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    NLUC3F10H
    

    Use

    Luciferase

    Tags

    Flag (x3) and His (x10)

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pETCK3 TGP-E

    Plasmid

    #236255

    Purpose

    Expresses TGP-E, a thermostable fluorescent protein

    Depositor

    Natasha DeVore

    Article

    Anderson et al Biochem Mol Biol Educ. 2024 Sep-Oct;52(5

    Insert

    Thermal Green Protein Q66E (TGP-E)
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    lac operon

    Available Since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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27. BglB in pET29b(+)

    Plasmid

    #55773

    Purpose

    Expresses beta-glucosidase

    Depositor

    Justin Siegel

    Article

    Carlin et al PLoS One. 2016 Jan 27;11(1):e0147596. do

    Insert

    Beta-glucosidase B (bglB Synthetic)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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28. pET28a-SpySrtA

    Plasmid

    #51139

    Purpose

    expression plasmid for N-terminal his tagged S.pyogenes SrtA

    Depositor

    Hidde Ploegh

    Article

    Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

    Insert

    S.pyogenes SrtA
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-80

    Promoter

    T7

    Available Since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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29. pET303-colE1T

    Plasmid

    #155180

    Purpose

    Expresses colicin E1 residues 1-190

    Depositor

    Joanna Slusky

    Article

    Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

    Insert

    colicin E1 residues 1-190
    

    Tags

    C-terminal 6x Histidine tag

    Expression

    Bacterial

    Mutation

    truncation of colicin E1 including 1-190

    Promoter

    T7

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pET28a-TRBP

    Plasmid

    #50351

    Purpose

    bacterial expression of TRBP

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

    Insert

    TRBP (TARBP2 Human)
    

    Tags

    His and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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