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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,418 results for: 683

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Showing: 921 - 960 of 4418 results

  1. pJRM919_WT aa 1-419 His POLI

    Plasmid
    #201445
    Purpose
    Low copy vector for expressing the catalytic domain (amino acids 1-419) of human DNA polymerase iota in E. coli with an N-terminal 6x His tag. C-terminal truncation mutant of pJRM919_WT His POLI
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    DNA polymerase iota (POLI Human)
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Expresses amino acids 1-449 of DNA polymerase iot…
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201445-plasmid-map-sequence-id-395334

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  3. lentiGuide-Crimson

    Plasmid
    #70683
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.
    Depositor
    Daniel Bauer
    Article
    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature
    Inserts
    S. pyogenes sgRNA cassette
    Crimson Reporter
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    Ef1-a and hU6
    Available Since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    70683-plasmid-map-sequence-id-217910

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  6. TFORF0028

    Plasmid
    #143683
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF44 (ZNF44 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143683-plasmid-map-sequence-id-407869

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  16. CL20_mEGFP_FOidr_131

    Plasmid
    #244683
    Purpose
    Express mEGFP-tagged intrinsically disordered protein (IDR), FOidr_131, derived from human fusion protein PAX5_BZW1; PAX5_CBFA2T3; PAX5_ESRRA; PAX5_FOXP1; PAX5_JAK2; PAX5_KANK1; PAX5_NCOA5; PAX5_NOL4L; PAX5_PAX5; PAX5_ZCCHC7; PAX5_ZNF276; PAX5_ZNF521; ZCCHC7_PAX5
    Depositor
    Richard Kriwacki
    Article
    Maiti et al Sci Adv. 2025 Dec 5;11(49):eady1420. doi
    Insert
    FOidr_131
    Tags
    monomeric EGFP
    Expression
    Mammalian
    Mutation
    Unmutated
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    244683-plasmid-map-sequence-id-488154

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  19. pCMV6-AN-DDK_S172A_M173A RAD23A

    Plasmid
    #201574
    Purpose
    Expresses a variant of human RAD23A containing mutations S172A and M173A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    UV excision repair protein RAD23 homolog A (RAD23A Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Contains mutations S172A and M173A
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201574-plasmid-map-sequence-id-395069

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  23. pCMV6-AN-DDK_C344A_F354A RAD23A

    Plasmid
    #201579
    Purpose
    Expresses a variant of human RAD23A containing mutations C344A and F354A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23A
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    UV excision repair protein RAD23 homolog A (RAD23A Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Contains mutations C344A and F354A
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201579-plasmid-map-sequence-id-395063

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  27. pCMV6-AN-DDK_WT RAD23B_delta aa 276-339 (delta XPC-binding domain)

    Plasmid
    #201547
    Purpose
    Expresses a mutant form of human RAD23B that lacks the XPC-binding domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    UV excision repair protein RAD23 homolog B (RAD23B Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Lacks residues 276-339
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201547-plasmid-map-sequence-id-395051

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  32. LSB-hsa-miR-625-5p

    Plasmid
    #103683
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-625-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-625-5p target (MIR625 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103683-plasmid-map-sequence-id-196414

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  39. pLenti-EF1a-GFP-miRE_shRen-Puro

    Plasmid
    #135683
    Purpose
    Constitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection
    Depositor
    Florian Karreth
    Insert
    shRenilla
    Use
    Lentiviral
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135683-plasmid-map-sequence-id-327605

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  40. pTWEEN-miR-224

    Plasmid
    #35683
    Depositor
    George Calin
    Insert
    nsa-mir-224 (MIR224 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 2, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 921 - 960 of 4418 results