We narrowed to 2,021 results for: D10

Showing: 461 - 480 of 2021 results

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pEF_dCas9

Plasmid

#68416

Purpose

Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A/H841A

Promoter

human EF1[alpha]

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223_ATG16L1_WT

Plasmid

#82255

Purpose

Gateway Donor vector containing ATG16L1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ATG16L1 (ATG16L1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRDA_078

Plasmid

#158582

Purpose

Lentiviral expression of BE4max and customizable gRNA

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

Cas9 D10A

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pKin1A

Plasmid

#31607

Depositor

Anthony Brown

Article

Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

Insert

kinesin-1A (Kif5a Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only

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Epsin2-pmCherryC1

Plasmid

#27673

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Epsin 2 (Epn2 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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SPACE (pRZ1813)

Plasmid

#140242

Purpose

CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

SPACE

Expression

Mammalian

Mutation

V82G in TadA*, D10A in Cas9, R187W in pmCDA1

Promoter

CMV

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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3XFLAG-VP64-SadCas9-NLS-VP64

Plasmid

#135338

Purpose

3xFLAG 2xVP64 SadCas9 for single guide cloning

Depositor

Ronald Cohn

Article

Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

Insert

SadCas9

Tags

3xFLAG-VP64-SV40 NLS and NLS-VP64

Expression

Mammalian

Mutation

D10A and N580A

Promoter

CMV

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-BE-zCas9

Plasmid

#101739

Purpose

expression of an optimized Cas9 for base-editing in zebrafish

Depositor

Shuo Lin

Article

Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

Insert

rAPOBEC1-XTEN-zCas9-UGI-NLS

Use

CRISPR

Expression

Mammalian

Mutation

D10A

Promoter

CMV

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

Plasmid

#185910

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

human codon optimized ABE8e-nSpCas9-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9=D10A

Promoter

CMV and T7

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLL7 lenti-dcas9-3xflag-blast

Plasmid

#112133

Purpose

lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

dCas9(D10A, N863A)-T2A-Blast

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A mutants in Cas9

Promoter

EF1a

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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LAMP-mCherry-CRY2

Plasmid

#102249

Purpose

Expresses fusion of CRY2PHR with mCherry and LAMP1 (1-415)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

LAMP-mCherry-CRY2 (LAMP1, CRY2 Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pRH2502

Plasmid

#84379

Purpose

Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

Depositor

Robert Husson

Article

Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

Insert

dcas9

Use

CRISPR

Expression

Bacterial

Mutation

Aspartate at position 10 changed to Alanine, Hist…

Promoter

uv15tetO

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-OptoSTIM1(Cry2(D387A))

Plasmid

#70160

Purpose

Light-insensitive mutant of OptoSTIM1

Depositor

Won Do Heo

Article

Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

Insert

OptoSTIM1(Cry2(D387A)) (STIM1 Human, Mustard Weed)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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ACBE-Q

Plasmid

#204608

Purpose

Mammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-Q

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

Insert

bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP

Use

CRISPR

Tags

6*His

Expression

Mammalian

Mutation

TadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-xFNLS-P2A-Puro

Plasmid

#110872

Purpose

Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xFNLS(3.7)

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pNICKclos1.0

Plasmid

#73639

Purpose

Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

Depositor

Sheng Yang

Article

Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

Inserts

Cas9 nickase
sGRNA to pyrE

Use

E.coli-clostridium shuttle vector

Mutation

D10A

Promoter

j23119 and ptb

Available Since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNICKclos2.0

Plasmid

#73228

Purpose

Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052

Depositor

Sheng Yang

Article

Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

Inserts

Cas9 nickase
sgRNA to xylR

Use

E.coli - clostridium shuttle vector

Mutation

D10A

Promoter

Pj23119 and Pthl

Available Since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG R2pH-LAMP1-3xFLAG

Plasmid

#157940

Purpose

expresses ratiometric sensor of lysosomal lumenal pH

Depositor

Massimiliano Stagi

Article

Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

Insert

R2pH-LAMP1-3xFLAG (Lamp1 Mouse)

Tags

3XFLAG, mCherry, and pHluorin

Expression

Mammalian

Promoter

Chick beta actin pomoter

Available Since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pScI_dCas9-CDA_J23119-sgRNA

Plasmid

#108550

Purpose

Bacterial Target-AID vector with sgRNA

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Inserts

dCas9-PmCDA1
Streptococcus pyogenes sgRNA

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

J23119 and lambda OR

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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LLP457 pGK-dCas9-Suntag-BFP

Plasmid

#100957

Purpose

BPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tag

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

dCas9, SunTag array (10xGCN4)

Use

CRISPR

Tags

3xHA and BFP

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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