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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,415 results for: ESP

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Showing: 461 - 480 of 14415 results

  3. tdTomato-CathespinB-6

    Plasmid
    #58075
    Purpose
    Localization: Lysosomes, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    CathespinB (Ctsb Rat)
    Tags
    tdTomato
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    58075-plasmid-map-sequence-id-209007

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  4. pCMV_NESPylRS(WT)_hU6tRNAPyl

    Plasmid
    #223509
    Purpose
    PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5
    Inserts
    NES Pyrrolysyl-tRNA Synthetase (pylS Methanosarcina mazei)
    tRNA Pyl
    Expression
    Mammalian
    Promoter
    CMV, SP6 and U6
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    223509-plasmid-map-sequence-id-448627

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  5. mEmerald-CathespinB-6

    Plasmid
    #54024
    Purpose
    Localization: Lysosomes, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    CathespinB
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. eSpCas9(1.1)_No_FLAG_NPM1_G6

    Plasmid
    #178091
    Purpose
    Expresses the NPM1 G6 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with NPM1_mNeonGreen_Donor to tag NPM1 with mNeonGreen. pX330-like plasmid.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    NPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178091-plasmid-map-sequence-id-350141

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  8. eSpCas9(1.1)_No_FLAG_ATP1A1_G3

    Plasmid
    #86611
    Purpose
    Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    86611-plasmid-map-sequence-id-168847

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  9. pX330-Flag-deSpCas9

    Plasmid
    #92114
    Purpose
    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    dead/inactive eSpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A, K848A, K1003A, R1060A
    Promoter
    Cbh
    Available Since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92114-plasmid-map-sequence-id-197615

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  10. DNA-CARtcr-IRESpuro

    Plasmid
    #89346
    Purpose
    2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassette
    Depositor
    Ron Vale
    Article
    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do
    Insert
    TCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag
    Use
    Lentiviral
    Tags
    SNAPf tag
    Expression
    Mammalian
    Promoter
    SFFV
    Available Since
    June 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    89346-plasmid-map-sequence-id-176306

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  12. mPlum-CathespinB-6

    Plasmid
    #55955
    Purpose
    Localization: Lysosomes, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Insert
    CathespinB (Ctsb Rat)
    Tags
    mPlum
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    55955-plasmid-map-sequence-id-173178

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  13. Dendra2-CathespinB-6

    Plasmid
    #57703
    Purpose
    Localization: Lysosomes, Excitation: 490 / 553, Emission: 507 / 573
    Depositor
    Michael Davidson
    Insert
    CathespinB
    Tags
    Dendra2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 16, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pLV V5-Nesprin-3

    Plasmid
    #175136
    Purpose
    Lentiviral expression of V5-tagged mouse Syne3
    Depositor
    Larry Gerace
    Insert
    Syne3 (Syne3 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175136-plasmid-map-sequence-id-352023

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  20. eSpCas9(1.1)_NoFlag_hROSA26_Left

    Plasmid
    #191442
    Purpose
    Expresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hROSA26 sgRNA
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191442-plasmid-map-sequence-id-378118

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Showing: 461 - 480 of 14415 results