We narrowed to 1,577 results for: MK

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pET21-pelB-LaM-2

Plasmid

#172768

Purpose

Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-2

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBirA-P19

Plasmid

#127919

Purpose

Transient expression of biotin ligase BirA and p19 in plant protoplast

Depositor

Silin Zhong

Article

Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

Insert

BirA

Expression

Plant

Promoter

35S

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z28

Plasmid

#120071

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xHA

Use

Pcr template

Tags

3xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P29

Plasmid

#120040

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-II

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Strep-II

Use

Pcr template

Tags

Strep-II

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-12

Plasmid

#119991

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet

Use

Pcr template

Tags

mScarlet

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSIL-eGFP-shACTN1

Plasmid

#52676

Purpose

shRNA against α-actinin-1 with eGFP transfection marker

Depositor

Johannes Hell

Article

Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

Insert

ACTN1 (Actn1 Rat)

Use

RNAi

Tags

CMV-EGFP

Promoter

U6

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pMTTH+eGFP-bromo1

Plasmid

#157805

Purpose

Expression of synthetic protein composed of eGFP and BRD4 bromodomain #1

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

eGFP-bromo1

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-actin-V159N

Plasmid

#118383

Purpose

Gateway compatible donor vector with actin-V159N.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Mutation

V159N

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMS07

Plasmid

#230070

Purpose

Inducible Promoter (rhamnose-induced, BT3763/rhaR system), replicates via RepA; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

Depositor

Sean Crosson

Article

Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Bacterial

Promoter

rhamnose-inducible (BT3763)

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMS03

Plasmid

#230937

Purpose

Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), replicates via RepA; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

Depositor

Sean Crosson

Article

Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Bacterial

Promoter

RpoD (bt_1311/BT_RS06635)

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pMS05

Plasmid

#230069

Purpose

Inducible Promoter (anhydrotetracycline-induced, tetO/tetR system), replicates via RepA; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

Depositor

Sean Crosson

Article

Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Bacterial

Promoter

anhydrotetracycline-inducible

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pMS02

Plasmid

#230066

Purpose

Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), integrates via IntN1; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

Depositor

Sean Crosson

Article

Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Bacterial

Promoter

RpoD (bt_1311/BT_RS06635)

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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FRB-polySH33

Plasmid

#175233

Purpose

Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMTTH+bromo5

Plasmid

#157803

Purpose

Expression of synthetic protein composed of 5 repeats of BRD4 bromodomain #1

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

bromo5

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pNP1 Aqua trigger

Plasmid

#107360

Purpose

Trigger sequence for pNP1 Aqua toehold switch

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold trigger 8

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCOM-CD63-COM

Plasmid

#138350

Purpose

Expressing COM-CD63-COM fusion protein in mammalian cells

Depositor

Baisong Lu

Article

Yao et al J Extracell Vesicles. 2021 Mar;10(5):e12

Insert

COM-CD63-COM fusion protein

Use

CRISPR and Lentiviral

Tags

COM is fused to the N and C termini of human CD63

Expression

Mammalian

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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ZIKV_Sensor_32B_LacZ

Plasmid

#75007

Purpose

Toehold switch sensor to detect Zika Virus

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_sensor_32B_LacZ

Tags

LacZ

Expression

Bacterial

Promoter

T7

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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shERK1a-mlpx-puro

Plasmid

#65228

Purpose

encodes a shRNA against ERK1

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

shRNA against ERK2 (MAPK1 Human)

Expression

Mammalian

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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T7-Phi6-P2

Plasmid

#182535

Purpose

Template for production of Phi6 P2 RNA

Depositor

Peter Sarin

Article

Gregorova et al MethodsX. 2022 May 23;9:101737. doi: 10.

Insert

P2 pol

Expression

Bacterial

Promoter

T7 promoter

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAM-PAT-ProCDKA;1::CDKA;1:YFP

Plasmid

#80685

Purpose

binary Expression vector for plant expression

Depositor

Nico Dissmeyer, Arp Schnittger

Article

Nowack et al Nature. 2007 May 17;447(7142):312-5. Epu

Insert

CDKA;1 (CDC2 Mustard Weed)

Tags

YFP

Expression

Plant

Promoter

ProCDKA;1

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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