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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,246 results for: Xpa

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Showing: 461 - 480 of 2246 results

  3. pFP.R265

    Plasmid
    #138167
    Purpose
    Expresses the split mCherry reporter interrupted by the SspDnaB M86 artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Publication
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(VDA).M86.(SDL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138167-plasmid-map-sequence-id-273521

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  7. pUDP293

    Plasmid
    #204228
    Purpose
    Episomal yeast plasmid expressing the Ercas12a and ErCas12a gRNA
    Depositor
    Jean-Marc Daran
    Publication
    Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d
    Inserts
    Eubacterium rectale cas12a (Ercas12a)
    BsaI_GFP drop-out_BsaI
    Use
    Synthetic Biology
    Tags
    NLS
    Mutation
    codon optimized for S. cerevisiae
    Promoter
    Saccharomyces cerevisiae PGK1 and Saccharomyces c…
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    204228-plasmid-map-sequence-id-408527

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  10. pYPQ166-iSpyMac

    Plasmid
    #149553
    Purpose
    Gateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMs
    Depositor
    Yiping Qi
    Publication
    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d
    Insert
    iSpyMacCas9
    Use
    CRISPR; Gateway compatible zispymaccas9 entry clo…
    Tags
    3X FLAG, NLS and NLS
    Expression
    Plant
    Mutation
    R221K, N394K, Protospacer interacting domain form…
    Available since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    149553-plasmid-map-sequence-id-306139

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  11. pCS2 xRor2-mCherry

    Plasmid
    #67162
    Purpose
    mammalian expression / in vitro transcription
    Depositor
    Alexandra Schambony
    Publication
    Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)
    Use
    In vitro transcription
    Tags
    mCherry
    Expression
    Mammalian
    Available since
    July 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pBad-CA TAG20

    Plasmid
    #105666
    Purpose
    expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 20 and C-terminal 6x his tag
    Depositor
    Ryan Mehl
    Publication
    Mehl et al 2020
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 20
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105666-plasmid-map-sequence-id-205513

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  15. pAS_4xMmaPylT(UUA)_EF1_MmaPylRS

    Plasmid
    #174896
    Purpose
    Mma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Publication
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Expression
    Mammalian
    Promoter
    EF1
    Available since
    Nov. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    174896-plasmid-map-sequence-id-344595

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  16. pET28a(+)::MBP-NL3F10H

    Plasmid
    #141291
    Purpose
    MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Publication
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    MBP-NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3), His (x10), and MBP
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    141291-plasmid-map-sequence-id-324616

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  20. ABE8r-R153del

    Plasmid
    #208296
    Purpose
    Expresses ABE8r in mammalian cells
    Depositor
    Weixin Tang
    Publication
    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA8r-R153del-SpCas9 D10A
    Expression
    Mammalian
    Mutation
    W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    208296-plasmid-map-sequence-id-410369

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Showing: 461 - 480 of 2246 results