We narrowed to 6,482 results for: itch

1. pZW6 (CK2alpha)

   Plasmid

   #27086

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2alpha (CSNK2A1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-GRK6

   Plasmid

   #32693

   Depositor

   Robert Lefkowitz

   Article

   Pitcher et al J Biol Chem. 1996 Oct 4. 271(40):24907-1

   Insert

   GRK6 (GRK6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-Padron2

   Plasmid

   #172355

   Purpose

   Expresses the reversibly switchable fluorescent protein Padron2 in bacterial cells

   Depositor

   Stefan Jakobs

   Article

   Konen et al ACS Nano. 2021 May 21. doi: 10.1021/acsn

   Insert

   Padron2
   

   Use

   Tags

   6xHisTag (N terminal on backbone)

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSP64-hERG1a

   Plasmid

   #53051

   Purpose

   Expresses alpha subunit of hERG1a K channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   Promoter

   SP6

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. AEB-No-aptamer-controlmRFP1

   Plasmid

   #63848

   Purpose

   This pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene. It should be used when testing AEB-F12 and AEB-Theo45 riboswitches to measure their non-specific ligand effect on mRFP1 expression.

   Depositor

   Howard Salis

   Article

   Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

   Insert

   No-aptamer control mRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   J23100

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAL149

   Plasmid

   #22275

   Purpose

   Expresses PhyB-mCherry with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   PhyB(1-908) (PHYB Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   mCherry-Kras4BCT (prenylation sequence)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL4.23-Fosprom

   Plasmid

   #188113

   Purpose

   Expresses luciferase under control of Fos promoter in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Shchuka et al PLoS One. 2023 Jan 3;18(1):e0271081. doi

   Insert

   Fos promoter (Fos Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Fos endogenous promoter

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-D2s-L-Venus

   Plasmid

   #19966

   Depositor

   Jonathan Javitch

   Article

   Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

   Insert

   D2 short variant-monomeric Venus (DRD2 Human, Aequorea Victoria)
   

   Use

   Tags

   Flag and monomeric Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIA1385

   Plasmid

   #166862

   Purpose

   Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

   Depositor

   Irina Artsimovitch

   Article

   Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

   Insert

   SARS-COV-2 Nsp12 (ORF1ab Synthetic)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAL204

    Plasmid

    #22282

    Purpose

    Expresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (PHYB Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CMV-hCas9-T2A-mStrawberry

    Plasmid

    #187224

    Purpose

    Expresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Inserted T2A-mStrawberry at 3' terminal of M…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-D2s-L-V1

    Plasmid

    #19967

    Depositor

    Jonathan Javitch

    Article

    Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

    Insert

    D2 short variant-N terminal Venus (DRD2 Human, Aequorea Victoria)
    

    Use

    Tags

    Flag and N terminal Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

13. LightR-iCre-miRFP670

    Plasmid

    #162158

    Purpose

    Expresses LightR-iCre-miRFP670 in mammalian cells

    Depositor

    Andrei Karginov

    Article

    Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

    Insert

    LightR-iCre-miRFP670
    

    Use

    Tags

    NLS and miRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-hCas9-T2A-miRFP670

    Plasmid

    #187223

    Purpose

    Expresses human Cas9 nuclease and miRFP670 via T2A linker under control of a CMV promoter.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    Cas9
    

    Use

    Tags

    Inserted T2A-miRFP670 at 3' terminal of MCS.

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-D2s-L-V2

    Plasmid

    #19968

    Depositor

    Jonathan Javitch

    Article

    Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

    Insert

    D2 short variant-C terminal Venus (DRD2 Human, Aequorea Victoria)
    

    Use

    Tags

    C terminal Venus and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

16. Myc-CK2beta 6KR (pAB1016)

    Plasmid

    #37361

    Depositor

    David Litchfield

    Article

    French et al J Biol Chem. 2007 Oct 5;282(40):29667-77

    Insert

    CK2beta 6KR (CSNK2B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K33R, K139R, K177R, K191R, K208R, K212R

    Promoter

    CMV-F

    Available since

    July 17, 2012

    Availability

    Academic Institutions and Nonprofits only

17. CNC3 (CASANOVA-C3)

    Plasmid

    #137191

    Purpose

    Expresses CNC3 (CASANOVA-C3), a blue light dependent NmeCas9 inhibitory protein

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2021 Mar 18;49(5):e29

    Insert

    CNC3 (CASANOVA-C3)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AEB-No-aptamer-controlluciferase

    Plasmid

    #63853

    Purpose

    This pFTV1 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-Dopa5, AEB-T4-2 riboswitches to measure their non-specific ligand effect on luciferase expression.

    Depositor

    Howard Salis

    Article

    Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

    Insert

    No-aptamer control mRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    tac

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. Nanog-t2A-mCherry

    Plasmid

    #127541

    Purpose

    Expresses the transactivation domain of mouse Nanog and mCherry via t2A linker.

    Depositor

    Jennifer Mitchell

    Article

    Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

    Insert

    Nanog (Nanog Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codes for the last 110 a.a of Nanog

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAG415-ADH1p-HA-rap1-K9R

    Plasmid

    #84531

    Purpose

    This plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeast

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    K(43,61,69,117,240,246,527,582,651)R

    Promoter

    ADH1

    Available since

    Jan. 17, 2017

    Availability

    Academic Institutions and Nonprofits only