We narrowed to 497 results for: transposase
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Plasmid#154016PurposeVector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposaseDepositorInsertmRFP1
ExpressionMammalianMutationH210PPromoterCAGAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
LiOn-CAG∞IRFP
Plasmid#154017PurposeVector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein IRFP670 from a CAG promoter upon action of the piggyBac transposaseDepositorInsertIRFP670
ExpressionMammalianPromoterCAGAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169
Plasmid#178528PurposeExpresses rtTA and SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cellsDepositorInsertrtTA, SspB-mScarlet-I-MYPT (1-169aa)
ExpressionMammalianPromoterCAG, Tet responsive elementAvailable SinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-Neo-TetO-Cdk12_Nterm_FlagHa
Plasmid#127179PurposeN-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vectorDepositorInsertN-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
UsePiggybac transposase vectorTagsFlag- HA- Tandem EpitopesExpressionMammalianPromoterTetO PromoterAvailable SinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-3xCTCF-TetO
Plasmid#213023PurposepiggyBac donor vector containing an 8-kb TetO operator array flanked by 3xCTCF sites for random integration using PBase transposaseDepositorInsert3xCTCF-TetO
UseMouse Targeting; Piggybase integration (transposo…Available SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBlue-ACTB-IR-IRES-Puro-pEF1α-EGFP-IR-ACTB
Plasmid#169921PurposeDNA donor plasmid for site-specific integration of IRES-Puro-pEF1a-EGFP cassette at the ACTB locus using Cas9-transposase fusion proteins.DepositorInsertIRES-Puro-pEF1a-EGFP
UseCRISPRPromoterEF1aAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-rBrokenheart
Plasmid#203394PurposeAAV transfer plasmid containing a constitutive (non Cre-dependent) BrokenHeart construct: a piggyBac donor transposon interrupting tdTomato. Transposase activity rescues coding sequence.DepositorInsertBrokenheart
UseAAVExpressionMammalianPromoterEF1aAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-empty-DSE-TetO-8kb
Plasmid#213022PurposepiggyBac donor vector containing an 8-kb TetO operator array for random integration using PBase transposaseDepositorInsertTetO
UseMouse Targeting; Piggybase integration (transposo…Available SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT
Plasmid#216763PurposePiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposaseDepositorInsertmCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
TagsEGFP and mCherryExpressionMammalianAvailable SinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-HALO-hLRRK2 (LIR1+2 mutant WEVL -> AEVA / WTFI -> ATFA)
Plasmid#229744PurposeExpresses Halo tagged human LRRK2 with LIR1 and LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.DepositorInsertLRRK2 (LRRK2 Human)
TagsHaloExpressionMammalianMutationLIR1+2 mutant WEVL -> AEVA / WTFI -> ATFAPromoterEF1AAvailable SinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWT-IFl_WT-IFr
Plasmid#192207PurposeWildtype I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.DepositorInsertWildtype I-F Leader and wildtype I-F Locus DNA
ExpressionBacterialAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pScr-IFl_WT-IFr
Plasmid#192208PurposeScrambled I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.DepositorInsertScrambled I-F Leader and wildtype I-F Locus DNA
ExpressionBacterialAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiOn*-CAG∞RFP
Plasmid#154019PurposeVector based on the LiOn integration-coupled translational switch and devoid of TTAA sequences, expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposaseDepositorInsertmRFP1
ExpressionMammalianMutationAll TTAA in vector backbone were replaced by sile…PromoterCAGAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
LiOn-CMV∞Cre
Plasmid#154018PurposeVector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing a functional Cre recombinase from a CMV promoter upon action of the piggyBac transposaseDepositorInsertCre-FLAG
ExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-mNeonGreen. PGK-puro
Plasmid#136255PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmNeonGreenExpressionMammalianAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-short_BrokenHeart (no BC)
Plasmid#86950PurposeAAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.DepositorInsertBrokenheart construct
UseAAVExpressionMammalianAvailable SinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2/GD-IRES-GFP-CTNNB1
Plasmid#192868PurposeCarries a Sleeping Beauty (SB) transposon vector that can be used to deliver an activated human CTNNB1(S33Y) transgene into cells, when a source of SB transposase is also co-introduced.DepositorInsertsluc+
EGFP
CTNNB1
ExpressionMammalianAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-IB-H2B-HaloTag
Plasmid#247345PurposeExpresses H2B-Halo under CAG promoter and can be randomly integrated by PiggyBac transposaseDepositorAvailable SinceNov. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1α-H2B-PA-mCherry-PGKneo
Plasmid#247340PurposeExpresses photoactivatable H2B-Cherry under EF1 promoter and can be randomly integrated by PiggyBac transposaseDepositorAvailable SinceNov. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
PB-EF1-H4-HaloTag-IRES-Neo
Plasmid#247450PurposeExpresses wild-type H4-Halo under EF1 promoter and can be randomly integrated by PiggyBac transposaseDepositorAvailable SinceNov. 10, 2025AvailabilityAcademic Institutions and Nonprofits only