We narrowed to 19,001 results for: Tec

1. c3GIC9

   Plasmid

   #62191

   Purpose

   col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes

   Depositor

   Lukas Dow

   Article

   Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

   Insert

   humanized S. Pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3x FLAG

   Promoter

   TRE3G

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTH1-SaSrtA

   Plasmid

   #64973

   Purpose

   Expresses the wild type of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59

   Promoter

   T7 promoter

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAM/CBA-eGFP-miRaSyn(human)x3-WPRE-bGHpA

   Plasmid

   #194247

   Purpose

   Expresses 3 miRNAs targeting human alpha-Synuclein

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   miRaSynuclein(human) (SNCA Human)
   

   Use

   AAV and RNAi

   Promoter

   CAG

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGWB508-cTP-sfGFP

   Plasmid

   #213863

   Purpose

   Binary vector for the expression of sfGFP flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

   Insert

   Plastid transit peptide fused sfGFP
   

   Expression

   Bacterial and Plant

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19/CF-mChe

   Plasmid

   #183166

   Purpose

   Construct for transient expression of cytoplasmic foci-targeted mCherry in plants.

   Depositor

   Qinlong Zhu, Yao-Guang Liu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   CF-mCherry
   

   Tags

   AtTZF1, A. thaliana TANDEM ZINC FINGER PROTEIN 1

   Expression

   Plant

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. UBQ10:sXVE:(MCS)-S11-DI-GFP1–9

   Plasmid

   #108260

   Purpose

   Binary vectors used for the inducible expression of dual intein-coupled tripartite split-sfGFP components in plants

   Depositor

   Tzu-Yin Liu

   Article

   Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Plant

   Promoter

   UBQ10:sXVE:

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPU-huTRIMmiR30-TRIM5

   Plasmid

   #132938

   Purpose

   All-in-one huTRIM5 rescue-TRIM5 shRNA knockdown

   Depositor

   Jeremy Luban

   Article

   Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

   Insert

   human TRIM5
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMM131

   Plasmid

   #127214

   Purpose

   BeYDV replicon with constitutive Luc expression, AtSTM, sgRNA targeting PDS

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   Luc, AtSTM, sgRNA
   

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. GB4080

   Plasmid

   #215249

   Purpose

   Module for the constitutive expression of EGFP, p19 and the N. nambi luminescence pathway genes fused to a SF.

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   SF-EGFP-p19-LUZ-H3H-HISPS-CPH
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTrex-Luc-Neo

    Plasmid

    #48336

    Purpose

    Trasgenically expresses firefly luciferase in T. cruzi with neomycin selection

    Depositor

    Rick Tarleton

    Article

    Sanchez-Valdez et al Elife. 2018 Mar 26;7. pii: 34039. doi: 1

    Insert

    firely luciferase
    

    Use

    Trypanasoma cruzi expression

    Promoter

    T. cruzi rRNA

    Available Since

    July 20, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. tracrRNA(scr)-CJ8421_04975 mRNA

    Plasmid

    #199718

    Purpose

    Expresses the Campylobacter mRNA CJ8421_04975 and tracrRNA with scrambled anti-repeat sequencesequence

    Depositor

    Chase Beisel

    Article

    Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

    Insert

    CJ8421_04975 mRNA and scrambled tracrRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. VPR_dCas9

    Plasmid

    #134601

    Purpose

    Express CHO codon-optimized dCas9 fused to VPR for CRISPR activation. 2A linked ZsGreen1 marker

    Depositor

    Lasse Ebdrup Pedersen

    Article

    Karottki et al Biotechnol Bioeng. 2020 Feb;117(2):593-5

    Insert

    VPR-dCas9-2A-ZsGreen-DR
    

    Tags

    VPR and ZsGreen1-DR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pH-A3A-PBE

    Plasmid

    #119774

    Purpose

    Conversion of C to T in rice plants transformation

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS-UGI-NLS
    

    Tags

    NLS (flanking UGI)

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    maize Ubiquitin-1

    Available Since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTK Lentivirus Multi Cassette Destination Vector (pAN2424)

    Plasmid

    #194221

    Purpose

    Backbone for constructing multi casette expression plasmids using the Mammalian Tookit

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC1430

    Plasmid

    #127981

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBJ3

    Plasmid

    #167132

    Purpose

    A kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    Frr promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAF

    Plasmid

    #104152

    Purpose

    Plasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.

    Depositor

    Robert Craigie

    Article

    Lee et al Proc Natl Acad Sci U S A. 1998 Feb 17;95

    Insert

    Barrier-to-autointegration factor (BAF) (BANF1 Human)
    

    Tags

    N-terminal His tag with thrombin cleavage site

    Expression

    Bacterial

    Available Since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBT100.106b

    Plasmid

    #122601

    Purpose

    clone APOBEC1 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    APOBEC1
    

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-Cre-6xHis

    Plasmid

    #62939

    Purpose

    Expression of Cre-6xHis in bacterial of cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cre recombinase
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. LbCas12a-D156R

    Plasmid

    #213744

    Purpose

    For circular RNA-mediated prime editor using LbCas12a-D156R in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    LbCas12a
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    D156R

    Promoter

    CMV

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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