We narrowed to 13,831 results for: Cas9

1. pJAT39

   Plasmid

   #204289

   Purpose

   For marking CRISPR HDR integrant with EGFP expressed in flight muscles.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   MHC-EGFP+H3:H26
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   MHC

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGNW4

   Plasmid

   #122088

   Purpose

   suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

   Depositor

   Pablo Ivan Nikel

   Article

   Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLH02

   Plasmid

   #117262

   Purpose

   Genome editing for Lactobacillus brevis ATCC367

   Depositor

   Sheng Yang

   Article

   Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

   Insert

   PorfX-LVISKB_1732-30 , sapK-sappR
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-Basic-8x-gRNA-eGFP

   Plasmid

   #60718

   Purpose

   eGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. BFP dest clone

   Plasmid

   #71825

   Purpose

   Lentiviral introduction of BFP reporter (Point mutation H66 in mEGFP results in BFP expression)

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. YN2_1_LT58_X2site

   Plasmid

   #177705

   Purpose

   Expresses Cas9 and sgRNA cassette for targeting site X2 in yeast Saccharomyces cerevisiae

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-Cry2FL-VP64

   Plasmid

   #60554

   Purpose

   Expresses Cry2FL-VP64 in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   Cry2FL-VP64
   

   Use

   Tags

   FLAG, HA tag, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-Universal

   Plasmid

   #52694

   Purpose

   This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

   Depositor

   Sebastian Lourido

   Inserts

   Cas9
   Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
   

   Use

   CRISPR; Toxoplasma gondii

   Tags

   3X FLAG and Nuclear localization signal

   Expression

   Mutation

   Promoter

   TgTUB1 and Toxoplasma U6

   Available since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJAT77

   Plasmid

   #204295

   Purpose

   pJAT plasmid with multiple cloning site for introducing custom payload. No U6 promoter. Restriction sites present to introduce flanking U6 promoters.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRB1017

    Plasmid

    #59936

    Purpose

    empty vector for gRNA cloning

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. LRG2.1-CyOFP1

    Plasmid

    #124771

    Purpose

    3rd generation lentiviral plasmid to co-express a guide RNA and CyOFP1

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFUGW NLS-Cre

    Plasmid

    #183425

    Purpose

    Lentiviral Cre vector

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    NLS-Cre
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJH372

    Plasmid

    #86839

    Purpose

    mamalian expression of AcrIIA1

    Depositor

    Joseph Bondy-Denomy

    Article

    Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

    Insert

    acrIIA1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYTK-DN6

    Plasmid

    #180287

    Purpose

    HygR multi-gene yeast expression vector (GFP-dropout)

    Depositor

    Andreas Milias-Argeitis

    Article

    Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

    Insert

    ConLS' - GFP - ConRE' - HygR - CEN6/ARS4
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. gRNA-eGFP-Reporter

    Plasmid

    #60719

    Purpose

    Expresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    gRNA (5'-AAAGGTCGAGAAACTGCAAA-3')
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and mouse U6

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYTK-DN5

    Plasmid

    #180286

    Purpose

    NatR multi-gene yeast expression vector (GFP-dropout)

    Depositor

    Andreas Milias-Argeitis

    Article

    Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

    Insert

    ConLS' - GFP - ConRE' - NatR - CEN6/ARS4
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCfB8644

    Plasmid

    #126919

    Purpose

    Plasmid containing integration cassette at IntC2 integration site in Yarrowia lipolytica for overexpression of XdCrtI and XdCrtYB

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI and HMG1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMiniT:GFP-Gephyrin TKIT donor

    Plasmid

    #169433

    Purpose

    Donor DNA for TKIT knockin of GFP to Gephyrin in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LRG2.1-mOrange

    Plasmid

    #124772

    Purpose

    3rd generation lentiviral plasmid to co-express a guide RNA and mOrange

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCfB8787

    Plasmid

    #126921

    Purpose

    Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of HMG1

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    HMG1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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